Secisbp2 (NM_029279) Mouse Untagged Clone

CAT#: MC222169

Secisbp2 (untagged) - Mouse SECIS binding protein 2 (Secisbp2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Secisbp2
• Synonyms: 2210413N07Rik; 2810012K13Rik; SB; SBP2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222169 representing NM_029279
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTCGGAGCGGCCGCGGGAGCCGGACGGCGAGGATAGCATCAAGTTGTCAGCTGATGTCAAACCAT
  TCGTCCCTAAGTTTGCTGGGCTCAACGTGGCGTGGTCAGAGTCCTCAGAGACACGTGTCTTCCCAGGCTG
  TGCGGCCACCTACTATCCATTTGTACAGGAACCACCAGCAGCTGAACAGAAAATGTATCCCGAAGACATG
  GCTTTCGGAGCCCCCACCTTTCCAGCACAGTACGTGTCTTCTGAGATAGCGCTGCATCCTTTTGCCTATC
  CCACTTACACCCTAGAGTCCGCACAGAGTGTTTGCTCAGTGCCAACCCTGCAGTACGACTACAGCCAAGC
  ACGGTGTCACCCAGGCTTTCGGACAGCAAAGCCCCGGCATGAGCACGTGTGCCCTCCACCTCAGGAAGCA
  AAAGGTGTATTTAAGAAAAAACCCTCTGATGAGAGAAGAGCATGTGAAGAGCAAAAGTCAAGCAGCAGAA
  GGGCTGACAATGCGGTGCCCTGTGAGGCGAGACCAGCCAGGGGGTCCAGTCACCTGTCCTCTCGAACTGA
  GAGCAGTTTGAAATCTGATGGTTACCACAAGCGACCCGACCGCAAGTCCAGAATCCTTGCGAAGAGTGCA
  TCTACCTCTAAACCTGAATTTGAGTTTAGTAGGTTAGACTTTCCTGAACTGCAGAGTCCAAAGAACAGTA
  ACATGCCAGAGACACAGAAGCCGCCCAGGTGGGGGCCTCTTGGCCCTGCTGCCAGTAACATGCCTCTCCT
  AGGAGACGTCGGCAAGCCCGTCGCAGATATGGTAGAGGGCAAAATGGTGAAGAGCGATCACACTGATGGA
  GCTGTGACCAGTAATGCCACTACCAGTTCCCCTTCATGTACCCAAGAGTTGTCTTGGACACCAATGGGTT
  ATATTGTTCGGCAGACAGTGTCTTCAGATTCAGCAGCAGCCACTGAAAATGTGACTTCCATGATAAACCT
  AAAGAAGACTACTTCATCAGCTGATGCTAAAAATGTTAGTGTGACATCTGAGGCTTTATCTTCAAATCCT
  TCCTACAACAGAGAAAAGCGTGTTTATCCTGCTCCAAAGGCCAAAGCATCACAAGGAGGTGAACTTGAAC
  AAAACGAAAGCTCCAAAAAGAATAAGAAAAAGAAAGAGAAGTCTAAACCGAGTTATGAAGTCCTGACGGT
  TCAGGAGCCGCCAAGGATTGAAGATGCAGAGGAATTCCCCAACCTGTCAGTTGCGTCGGAAAGAAGACAC
  AGAGGGCAATCACCGAAGCTTCACAGTAAACAGCAGACGCAGAATGAATTTAAAACAAGTGGGAAGAAGA
  GCCAGGTCCCAGTGCAGCTGGACCTGGGGGGCATGCTGGCCGCGCTGGAGAAGCAGCAGCAGCAGCAGCA
  CGCCTCGCACGCCAAGCCATCCTCCAGACCCGTCGTGTTCTCAGTTGGAGCAGTGCCAGTCCTGTCCAAG
  GATGCCTCCTCCAGTGAGAGGGGACGCCGCTCCAGTCAGATGAAGACCCCACACAACCCCCTGGACTCCA
  GTGCCCCCCTGATGAAGAAGGGGAAGCAGAGGGAGATACCTAAGGCCAAGAAGCCCACCTCACTGAAGAA
  GATAATTTTGAAAGAACGGCAAGAGAGGATGCAGCAGCGACTCCAAGAAAGTGCTGTGAGCCTGACGGTG
  GCCAGTGATGACTCACAGGATGTGGAGAGTGGCGCCAGTAACCAAACCCCCAGTCAGGACAACCCCACAG
  GTCCAGAGAAGACAGAAGAATCAGTGTCTTCTACACCTGTGGTTGAGGGTGAGTCAGAGGAGCCAGCTGG
  CACAGAGTTCCAGAGGGACCCAGAGGCTTGCCAGCCTGCCCCTGACAGTGCCACCTTCCCCAAGATCCAC
  AGCCGGAGGTTCCGGGACTACTGCAGCCAGATGCTTAGTAAAGAAGTAGATGCTTGTGTCACGGGTCTGC
  TCAAGGAGCTGGTGCGCTTCCAAGACCGCATGTACCAGAAGGATCCCGTCAAGGCCAAGACAAAACGGCG
  GCTCGTGCTGGGGCTGAGGGAGGTCCTGAAACACCTGAAGCTCAGGAAGCTGAAGTGCATCATCATCTCT
  CCCAACTGTGAGAAGACCCAGTCTAAAGGTGGGCTGGACGACACGCTGCACACCATCATCGACTGCGCCT
  GCGAGCAGAACATCCCCTTCGTGTTTGCACTCAATCGCAAGGCTCTGGGGCGGAGCCTGAATAAAGCTGT
  TCCTGTCAGCATTGTAGGGATCTTCAGCTACGATGGGGCCCAGGACCAGTTCCACAAGATGGTTGAGCTG
  ACCATGGCAGCCCGTCAGGCATACAAGACCATGTTGGAGACAATGCGGCAGGAACAGGCAGGAGAACCTG
  GACCTCAGTCCCCTCCCAGCCCACCCATGCAAGACCCCATCCCATCCACGGAAGAAGGCACCCTCCCTTC
  CACTGGAGAAGAGCCACACTACATTGAGATTTGGAAAAAGCACCTGGAAGCGTACAGTCAGCGTGCCCTG
  GAGCTGGAAGACTCACTGGAGGCGTCAACCTCTCAGATGATGAACTTGAATTTATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_029279
• Insert Size: 2577 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029279.2, NP_083555.1
• RefSeq Size: 3327 bp
• RefSeq ORF: 2577 bp
• Locus ID: 75420
• Cytogenetics: 13 A5
• Gene Summary: The incorporation of selenocysteine into a protein requires the concerted action of an mRNA element called a sec insertion sequence (SECIS), a selenocysteine-specific translation elongation factor and a SECIS binding protein. With these elements in place, a UGA codon can be decoded as selenocysteine. The gene described in this record encodes a nuclear protein that functions as a SECIS binding protein. Mutations in a similar gene in human have been associated with a reduction in activity of a specific thyroxine deiodinase, a selenocysteine-containing enzyme, and abnormal thyroid hormone metabolism. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2015]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).