Taf4b (NM_001100449) Mouse Untagged Clone

CAT#: MC222162

Taf4b (untagged) - Mouse TAF4B RNA polymerase II, TATA box binding protein (TBP)-associated factor (Taf4b), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Taf4b
• Synonyms: 105kDa; 2610524B04Rik; 4932409F03Rik; AW987595; Taf2c2; TAFII-105; TAFII105
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222162 representing NM_001100449
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGCGGGCCTCACGGAGCCGGCCGGCGCCGCTGCTCCTGTAGTCTCGAGCGCGTCGGGGGCCGTGA
  CCATGGCTCCGGTCGCCGCGCTGCCCGTCCGGGTGGAGGGCACCCCGGTGGCCCTGGGCCCTGTGACTAA
  GGCTCCTGTCAGTGTTTGCGTGGAGTCTGTGGCGCCCCAGCCCTTGCCAGCCCCAGTGGGGACTCTAGTG
  ACCAAAGTGGTTCCTGTCACTGCCCTTCCTAAACTCGGCAGCCCTAGGCTGCCCGCTCCTCAGATAGTCA
  CCGTGAAAACGCCGGGCACCACGACAATCCAACTACCGGCTAATTTGCAGCTTCCTCCAGGAACAGTTTT
  GATCAAAAGTAACAGTGGTCAGTTGATGCTGGTATCGCCTCAGCAAGCTGTAACAGGAGCCAAGACCACA
  AGTAACATAACTCCAAGACCAGCAGTACCAGCAAACACTCAAACAGTAAAGATCTGTACAGTGCCGAATT
  CTAGCTCACAATTAATGAAGAAAGTAACAGTGGCGCCTGTTAAAAATTTGACACAGATAGGAACTACTGT
  GGCAACCACTGCCTCCAGTACTTCCTCAGGACAACCTGTGGCTCTACCAAGCAGTGTCATCACAGTTACT
  CCTGCAAAGCTGGTGAATACTGTATCCACCCTGAAGTCATCAAGTTTGGGAGTGTTATCTACCCCCTCAA
  ATGACGCACGTCTCAAAGCTGAGACTTCAGTAGCTGCTCAGACTGCTCTTCCTCCGACAGTGCTAGAAAA
  TGTGAAAAAATGTAAAAACTTCCTTTCAATGTTAATAAAACTAGCATGTAGTGGATCACAGTCTCCAGAA
  ATGGGGCAGAATGTGAAGAGGCTGGTGGAGCAGCTTTTGGATGCAGAAATTGAAGCAGAAGAGTTTACTA
  GGAAACTGTATATTGAACTCAAGTCTGCACCTCAGCCGCACCTCGTCCCTTTTCTTAAGAAGAGTGTGGT
  TGCCCTAAGACAGCTTCTTCCAAATTCCCAGAGCTTCATTGAGAACTGTGTGAAGGAAGTTTCTGGTGAT
  GTGGTCATTTCCTCCTGTACCATGACAACAGCAACGTCTCCTGTGGTGACAAGTACAGTTTCCCCAGTCC
  TTGTGTCTGGAGCAACAGCACCCAGAACTCTGTCTGTTCAGCAGACCCTGAATCCACTTGCTGGTCCAGG
  AGTAGCAAACACTGGAGTTGTGACACTGCATTCTGTTGCCCCAGCTGCTGCAACAGGAGGTACGACAGCT
  GCAACTGTTTTGCTTCAAACTTCAAAACCACTAACAACATCAGTACCAAATACAGTGGCAGCAGTGTCCC
  TTCAGCCTGAGAATCCAGTTGTGTCTGGGGCAGCAGTAACACTGGCTATTCCATCAGCAACTTTTGGAGA
  AGCGTCCGCTACACCTCTTTGTCTTCCATCTGCGAAGCCTGCCATTACCTCTGCTGGGACCAAAGCTGAT
  AAACCTGCCATTGGCACTCCAGTCCAGATCGTTACACAACCGAGCACCCTCCTTCCACAGGCGGCTGGCA
  TTCCACAGACAGCCAAAGTCAAGCAACTAGTTGTACAGCAGCCTTCAGGAAGCAGTGTGAATCATGTGAC
  CTCCATTTCCCATTCCTCACCATTGAGCACTCAGAACTGTGGGCAGAAGACACCAGTAAATGCTGTGATG
  CCTACTAGTAAGTTTCCTCCATCTTCCATCATAAAGCAAATTACTCTGCCTGGAAATAAACTTTTGTCGC
  TTCAAGCTCAAAGATCTTCTATTCAGAGCAATAAAATAAAAGAGAATGGACCAACATGCTTCAGAGGTGA
  AGATGACATCAATGATGTGACTTTCATGGCAGAGGTCAACCTTGATGAAGAAAATGCCTGCATCTTAGCA
  GCACATTCTGACTTTGTTGGCACACTTATTCAGTCATGTAAAGAAGAACCATTCCTTGTCATTGGAGCTC
  TACAGAAGAGAATTTTAGACATTGGTAAAAAGCATGACATTACAGAACTTAACTCTGATGCTGTGAACTT
  GATCTCCCATGCAACACAGGAGAGATTACGAGGCCTTCTAGAGAAACTAACTACAATTGCTCAGCATCGA
  ATGACTATTTACAAGGGAAGTGAAAATTATATCTTGTCTACTGATACCAGATCACAGCTCAAATTTCTTG
  AAAAGCTCGATCAGTTGGAGAAACAGAGGAAGGATTTAGAAGAAAGAGAGATGTTACTAAAGGCAGCCAA
  GAGTCGCTCCAATAAAGAAGATCCAGAGCAGCTGAGATTAAAACAAAAAGCCAAAGAGTTGCAGCAGTTG
  GAGCTTGCCCAGATCCAGTACAGAGATGCTAATCTCACAGCTCTTGCAGCTATTGGACCAAGGAAGAAGA
  GACCACTAGAATCAGGAAATGAGAGCTTCAAAGATAACCCTTCTACTTCTGGGACTTCCAGCCTAACAGC
  CACCAAACCATTTCGTCCAAGAATCACAAGAATCTGCCTCAGGGATTTGATATTCTGTATGGAACAGGAA
  AGAGAGATGAAGTATTCTCGAGCCCTATACCTTGCCCTTCTTAAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001100449
• Insert Size: 2568 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001100449.1, NP_001093919.1
• RefSeq Size: 5149 bp
• RefSeq ORF: 2568 bp
• Locus ID: 72504
• UniProt ID: G5E8Z2
• Cytogenetics: 18 A1
• Gene Summary: Cell type-specific subunit of the general transcription factor TFIID that may function as a gene-selective coactivator in certain cells. TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators asond repressors. TAF4B is a transcriptional coactivator of the p65/RELA NF-kappa-B subunit. Involved in the activation of a subset of antiapoptotic genes including TNFAIP3. Through interaction with OCBA/POU2AF1, acts as a coactivator of B-cell-specific transcription. Plays a role in spermiogenesis and oogenesis.[UniProtKB/Swiss-Prot Function]