Arhgef15 (NM_177566) Mouse Untagged Clone

CAT#: MC222105

Arhgef15 (untagged) - Mouse Rho guanine nucleotide exchange factor (GEF) 15 (Arhgef15), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Arhgef15
• Synonyms: D130071N09; D530030K12Rik; E5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222105 representing NM_177566
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGCACAGTCTCTTCCTGCAGCAACGCCCCCTACCTTGAAGCCCCCTCGGATCATCCGCCCCCGAC
  CCCCATCTCGGCATAGGGCTCCACACTCCCCGGGGCCTCTCCACAATGGCTCCTCTCCGAAAGCACTTCC
  TCAAATATCCAATGATGCATCGGCCTCAGTGTGCACCTCCATCTTCTGGGAGCCCCCCACGGCTTCCCTG
  AAGCCCCCTGCTCTTCTGCCTCCCTCAGTTTCTAGAACCAGCCTGGACTCTCAGACTTCCCCTGACTCAC
  CTTCCAGCACACCGAGTCCCAGCCCAGTGTCCCGGCGCTCCATCTCCCCAGAACCAGCTCCCTGCTCTCC
  GGTGCCCCCACCCAAGCCCTCTGGGTCTTCCCGAACACCTCTGCCCTCGGGCCCTACGCCTCTCCAGGAT
  GGCTCTGCTTCGGCCCCTGGCACGGTTCGTAGACTGGCCGGCAAGTTTGAATGGGGAGCTGAAGGCAAAG
  CCCAGTCCTCAGACTCCCTGGAACGGTGTTCCCAAGGGAGCACGGAAGTGAATGGGGAGAAAGAGACTCC
  CGAAGCCGCCCTTTCTGGGAATGGGTCCCAGGAGAACGGCACTCCCGATGCTGCCCTGGCCTGCCCTCCA
  TGCTGCCCCTGTGTCTGCCATGTAGCAAAGCCTGGCCTGGAGCTCCGGTGGGTGCCTGTGGGGAGTTCTG
  AGGACATCCTCAGGATCCCCTGCCGGGCATCCCCACTCCGAGCCTCCCGCTCCCGCATCAATCCTCCAGT
  CATCAGTCACCCTCCAGTTGTGCTCACGTCCTATCGCTCCACTGCCGAGCGCAAACTCCTACCTCCCCTC
  AAGCCCCCCAAGCCAACTAAAGTCAGACAGGATATCAGCACCTCTGAGGAGCTTCCACAGCCAGATCTGA
  AACTGCCTTCAGAAGATGGGATCCAAACTGCTACAAAGGCCTGGGAAGGGGACAGACCTGAAGGAGCTCC
  TCTGAATGCTCCCCCTGTAGCCTTGGAGGGAAGGGAGGAGGAAGGATTGGATGGGCTGAAGGGGCTGCAG
  TGGGAACTGCCCCTGCAGGACGAACCTTTATACCAGACTTACCGAGCAGCTGTGCTGTCAGAGGAGCTGT
  GGGGTGTGGGTGAGGATGGGGGTCCCTCTCCAGCTAATCCTGGAGAAGCTCCCACCTTCTCCAGGCTCCC
  TGGACCTCGAAACACTCTGTGGCAGGAGCTCCCGGCTGTGAGAGGCAGTGGCCTCTTAGAGAGTCTAAGC
  CCCCAGGAGCGGCGCATGCAGGAGAGCCTGTTTGAGGTGGTGACATCAGAGGCCTCCTACCTGCGATCCC
  TGAGGCTGCTGACCGACACCTTCGTCCTGAGCCAGGCTCTCCGGGACACACTCACGCCTCGGGATCATCA
  TACGCTGTTCTCCAACGTGCAGCGAGTCCAGAGCGTCAGTGAGCGGTTTCTAGGAACACTGCTGTCTCGT
  GTTCGTTCCTCCCCACACATCACTGACCTGTGTGATGTGGTGCATGCCCATGCAGTTGGTCCTTTCTTCG
  TGTATGTGGATTATGTTCGGAACCAGCAGTATCAGGAGGAGACCTATAGCCGCCTTATGGATACAAACGT
  GCGCTTCTCCGCAGAGCTGCGAAGGCTGCAGAGCCTCCCCAAATGTGAGCGGCTCCCGCTGCCATCTTTC
  CTGCTGCTGCCCTTCCAGCGCATCACCCGGCTGCGGATGCTGTTGCAGAATATCCTAAGCCAGACAGAAG
  AGGGGTCCAGTCGTCAGGAGAACGCCCAGAAGGCTCTGGGTGCTGTGAGCAAGATCATTGAGCGCTGCAG
  CGCTGAGGTGGGGCGCATGAAGCAGACGGAAGAGCTGATACGGCTCACCCAAAGGTTGCGCTTCCACAAA
  GTCAAGGCTCTACCCCTGGTCTCCTGGTCACGGCGCCTGGAATTGCAGGGGGAGCTGACCGAGCTGGGGT
  GTCGGAGGGGGGGTGTGCTCTTCACCTCCCGTCCACGCTTCACTCCCCTCTGCCTACTGCTCTTCAGTGA
  CCTGCTGCTTATCACTCAGCCCAAGAGTGGGCAGCGGCTGCAGGTTCTGGACTATGCCCATCGCTCCCTG
  GTGCAGGCCCAGCAGGTTCCTGACCCATCTGGACCCCCTACTTTCCGCCTCTCGCTTCTCAGCAACCACC
  AGGGCCGCCCCACCCACAGGCTACTCCAAGCGGCCTCACTATCAGACATGCAGCGCTGGCTGGGAGCCTT
  CCCCACCCCAGGTCCCCTTCCCTGTTCCCCAGACACCATCTATGAGGACTGTGAGTGTTCCCAGGAGCTA
  TGTTCAGAGCCATCCACACCTTCCAAGACTGAGGGGCAAAGTCTGGAGTCCAAGGCCCCCCGAAAGCATC
  TGCACAAGAATCCAGAAGGTTGGCTTAAGGGGCTTCCTGGAGCCTTCCCTGCCCAACTGGTGTGTGAAGT
  CACAGGAGAACACGAGAGGAGGAAGCATCTTCGCCAGCACCAGAAGCTCCTTGAGGCTGTAGGACCCTCT
  TCAGGCACTCCTGACACCCCTCAACCTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_177566
• Insert Size: 2550 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177566.3, NP_808234.2
• RefSeq Size: 4213 bp
• RefSeq ORF: 2550 bp
• Locus ID: 442801
• UniProt ID: Q5FWH6
• Cytogenetics: 11 B3
• Gene Summary: Specific GEF for RhoA activation. Does not activate RAC1 or CDC42. Regulates vascular smooth muscle contractility. Negatively regulates excitatory synapse development by suppressing the synapse-promoting activity of EPHB2.[UniProtKB/Swiss-Prot Function]