Osbpl8 (NM_001003717) Mouse Untagged Clone

CAT#: MC222080

Osbpl8 (untagged) - Mouse oxysterol binding protein-like 8 (Osbpl8), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Osbpl8
• Synonyms: AA536976; AA536995; C730029P18Rik; D330025H14Rik; OR; ORP-8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222080 representing NM_001003717
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTCAGCGCCAAGGCAGAGACGCTAACCCAACCCCAACTAGAGATCTGCCTCAGCCTTCTCTCAGCC
  CCGCCAGCCTTCACAGCCAGGGTTTTGAAAGAGGAAAAGAAGACATTTCTCAAAATAAAGATGACTCTTC
  ACTTTCAATGTCAAAGAGCAAGTCTGAATCTAAACTATATAATGGCTCAGAAAAGGACAGTTCAACTTCA
  AGTAAACTCACGAAGAAAGAGTCTCTTAAGGTACAAAAGAAAAACTACCGTGAAGAGAAGAAGAGAGCCA
  CGAAGGAGTTACTCAGCACCATCACAGACCCCTCTGTCATTGTCATGGCCGACTGGCTGAAGATTCGTGG
  TACCCTGAAGAGCTGGACCAAGCTGTGGTGTGTGTTGAAACCTGGCGTGCTTCTGATCTATAAAACACAA
  AAAAATGGTCAGTGGGTCGGAACAGTCCTTCTGAATGCTTGTGAGATCATTGAACGTCCATCAAAAAAGG
  ATGGCTTTTGTTTCAAGCTTTTCCATCCTTTGGAGCAGTCTATTTGGGCAGTGAAGGGTCCTAAAGGTGA
  AGCGGTTGGCTCCATAACCCAGCCTCTACCTAGCAGCTACTTGATTATCCGAGCTACTTCAGAGTCAGAT
  GGAAGGTGCTGGATGGATGCTTTGGAGTTGGCTTTGAAGTGTTCCAGTCTCCTTAAGCGGACCATGGTGA
  GGGAGGGGAAGGAGCACGACCTGAGCATCTCCTCAGACAGCACACACGTGACGCTGTATGGCTTACTGCG
  CGCTAACAATCTCCACAGTGGCGACAACTTCCAGTTGAATGACAGTGAGATCGAAAGACAGCACTTTAAG
  GACCAAGATTTGTACTCTGATAAATCTGACAAGGAGAACGACCCGGAGCACGATGAGTCTGACAACGAGG
  TGCTGGGGAAGAGCGAGGAGAGCGACACAGACACGTCCGAAAGGCAAGACGACTCGTACATCGACCCTGA
  GCCTGTGGAGCCCTTAAAGGAGACCACCTACATGGAGCAGAGCCACGAAGAACTGGGAGAGGCAGGTGAG
  GCCTCTCAAACGGAAACTGTGTCTGAAGAAAACAAAAGCCTTATCTGGACGCTGCTCAAGCAAGTCCGTC
  CCGGCATGGACCTATCCAGGGTGGTTCTTCCTACGTTTATTTTGGAGCCCCGCTCTTTCCTGGATAAGCT
  TTCAGATTACTACTACCACGCCGATTTCCTGTCTGAAGCTGCTCTAGAAGAAAATCCGTATTTCCGTTTG
  AAGAAAGTCGTGAAATGGTATTTGTCAGGATTTTATAAGAAGCCAAAGGGACTAAAGAAACCATATAATC
  CTATACTTGGTGAGACTTTCCGATGTTTATGGATTCATCCCAGAACAAACAGCAAAACTTTCTATATTGC
  TGAACAGGTGTCCCATCACCCACCAATATCTGCCTTTTATGTCAGTAACCGGAAAGATGGGTTTTGCCTT
  AGTGGTAGCATCCTGGCTAAGTCCAAGTTTTATGGAAACTCGTTATCTGCCATATTGGAGGGAGAAGCCC
  GGTTAACTTTCTTGAACAGAGGTGAAGATTACGTCATGACAATGCCATATGCTCACTGTAAAGGAATTCT
  TTATGGCACAATGACCCTGGAGCTTGGTGGAACTGTCAATATTACCTGTCAGAAGACTGGATACAGTGCG
  ATACTTGAATTTAAACTAAAGCCATTTCTAGGGAGTAGTGATTATGTTAACCAAATATCAGGGAAACTTA
  AATTGGGAAAAGAAGTCCTAGCTACTTTGGAAGGCCATTGGGATAGTGAAGTTTTCATTAATGACAAAAA
  GACTGATAACTCAGAGATTTTCTGGAATCCAACTCCTGACATTAAACAGTGGAGATTAATAAGGCACACT
  GTAAAGTTTGAAGAACAGGATGATTTCGAATCAGAGAAACTCTGGCAACGAGTGACAAAAGCTATAAATG
  CCAAAGACCAAACGGAAGCTACTCAAGAAAAGTATGTTCTGGAAGAAGCTCAAAGACAAGCTGCCAGAGA
  TCGAAAAACAAAAACTCAAGAGTGGGTTTGTAAGTTATTTGAACTTGACCCACTCACTGGAGAATGGCAC
  TATAAGTTTTCAGATACTCGACCATGGGACCCACTTAATGATATGATACAATTTGAAAAAGATGGAGTTA
  TCCAGACCAAAGTGAAACATCGCACTCCAATGGTTAGTGTTCCAAAAATGAAACATAAACCAACAAGGCA
  GCAGAAGAAAGTGGTAAAAGGCTATTCTTCCCCAGAACCTGACATCCAGGATTCCTCTGGAAGTGAAGCT
  CAATCTGTAAAACCAAGTACACGAAGGAAGAAGGGGATAGACCTGGGAGACATTCAGAGTTCCATTGAGT
  CTATAAAGCAGACACAGGAAGAGATTAAAAGAAATATTATGGCTCTTCGAAATCATTTACTTTCAAGTAC
  ACCTGCTACAGATTATTTTCTGCAACAGAAAGACTACTTCGTCATTTTCCTTCTGATTTTGCTTCAAGTC
  ATAATAAACTTTATATTTAAGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001003717
• Insert Size: 2544 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001003717.1, NP_001003717.1
• RefSeq Size: 7115 bp
• RefSeq ORF: 2544 bp
• Locus ID: 237542
• UniProt ID: B9EJ86
• Cytogenetics: 10 D1
• Gene Summary: This gene encodes a member of the oxysterol-binding protein (Osbp) family, a group of intracellular lipid receptors. Like most members, the encoded protein contains an N-terminal pleckstrin homology domain and a highly conserved C-terminal Osbp-like sterol-binding domain. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) lacks an alternate exon compared to variant 1, resulting in the use of a downstream in-frame start codon compared to variant 1. Variant 2 encodes isoform b, which has a shorter N-terminus compared to isoform a. Sequence Note: This RefSeq record was created using sequences from two different strains, C57BL/6J and C57BL/6J x DBA/2J F1, because no single transcript from the same strain was available for the full length of the gene.