Vps41 (NM_172120) Mouse Untagged Clone

CAT#: MC222075

Vps41 (untagged) - Mouse vacuolar protein sorting 41 (yeast) (Vps41), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Vps41
• Synonyms: AI317346; mVam2; Vam2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222075 representing NM_172120
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAAGCAGAGGAGCAGGAAACCGAGTCTCTTGAAGAATCTACGGATGAGTCAGAGGAAGAAAGTG
  AAGAGGAACCCAAACTGAAGTATGAAAGGCTTTCCAATGGGGTGACAGAGATTCTTCAGAAGGATGCAGC
  AAGCTGTATGACGGTCCATGACAAGTTTTTGGCACTGGGAACACATTATGGCAAGGTTTATTTACTTGAT
  GTCCAGGGAAACATCACTCAGAAGTTTGATGTAAGTCCTGTGAAGATAAACCAAATTAGCTTGGATGACA
  GTGGAGAACACATGGGAGTGTGTTCAGAGGATGGCAAGCTGCAGGTCTTTGGGCTGTATTCTGGAGAAGA
  ATTTCACGAGACTTTTGACTGTCCCATTAAAATCATTGCTGTGCACCCACAGTTCGTGAGATCCAGTTGT
  AAGCAGTTTGTGACTGGCGGGAAAAAGCTGCTGCTGTTTGAACGGACCTGGATGAACAGATGGAAGTCTT
  CTGTGCTACACGAAGGGGAAGGAAACATAAGGAGTGTGAAGTGGAGAGGCCATCTGATTGCTTGGGCCAA
  CAATATGGGTGTGAAGGTTTTTGACATCACCTCAAAGCAAAGAATCAGCAATGTGCCCCGAGATGACATA
  AGTCTTCGCCCAGACATGTACCCCTGCAGCCTCTGCTGGAAGGACAATGTGACACTCATTATTGGCTGGG
  GAACTTCCATCAAGATATGCTCAGTGAAGGAACGCCACGCCAGTGAGATGAGGGATCTGCCAAGCCGATA
  TGTTGAGATAGTGTCTCAGTTTGAAACTGAATTCTACATTAGTGGACTTGCACCTCTCTGTGATCAGCTT
  GTTGTACTTTCCTACGTAAAGGAGGTTTCAGAAAAAACGGAAAGAGAATACTGTGCAAGACCTAGACTGG
  ACATCATCCAGCCACTTCCGGAGACTTGTGAAGAGATCTCCTCCGATGCTTTGACAGTCAGAGGCTTTCA
  GGAGAATGAATGTAGGGATTACCACTTAGAATACTCCGAGGGGGAATCACTCTTCTATGTCGTGAGTCCA
  AGAGATGTCGTCGTAGCCAAGGAACGAGACCAGGATGATCACATCGATTGGCTCCTTGAGAAGAAGAAAT
  ATGAAGAAGCTTTGATGGCTGCTGAAATTAGCCAAAGGAACATTAAACGACATAAGATTCTGGATATTGG
  GTTGGCATATGTAAACCACCTGGTGGAGAGAGGAGAGTACGACATGGCTGCAAGAAAATGCCAGAAAATT
  CTTGGGAAAAATGCATCCCTCTGGGAATACGAAGTTTACAAATTTAAAGAAATTGGGCAGCTTAAAGCAA
  TTAGTCCCTATTTACCTCGAGGGGACCCAGTTCTGAAACCACTCATCTATGAAATGATTTTGCACGAATT
  TCTGGAAAGTGACTATGAGGGCTTTGCCACATTGATCAGAGAGTGGCCTGGAGATCTATATAACAATTCC
  GTGATAGTTCAGGCAGTTCGAGACCACCTGAAGAAGGACAGTCAGAACAAAACGTTATTGAAAACACTGG
  CAGAATTGTACACCTATGACAAAAATTATGGCAATGCTCTGGAAATATACTTAACGTTAAGACATAAAGA
  TGTTTTCCAACTGATACACAAGCATAATCTTTTCAGCTCCATCAAGGATAAAATTGTGTTATTAATGGAT
  TTTGATTCAGAGAAAGCTGTTGACATGCTTTTGGACAATGAAGATAAAATTTCAATTAAAAAAGTGGTAG
  AAGAATTAGAAGACAGACCAGAACTGCAGCATGTGTATTTGCACAAGCTCTTCAAGAGAGACCATCATAA
  GGGACAGCGCTACCATGAAAAGCAGATCAGTCTGTATGCCGAGTATGACCGACCAAACCTGCTTCCCTTT
  CTCCGAGACAGTACCCATTGCCCACTTGAAAAGGCACTTGAGATCTGTCAACAAAGAAACTTTGTAGAAG
  AGACAGTTTACCTTCTGAGCCGAATGGGAAACAGTAGAAGTGCCCTCAAGATGATTATGGAGGAATTGCA
  TGATGTTGATAAAGCAATTGAGTTTGCCAAGGAACAAGATGATGGAGAGCTCTGGGAAGATCTGATTCTA
  TACTCCATCGACAAACCACCATTTATCACTGGCTTGTTGAACAACATTGGCACACATGTGGACCCGATTC
  TACTTATCCACCGAATTAAGGAAGGAATGGAGATTCCCAATTTGAGAGATTCCTTGGTTAAAATTCTGCA
  AGATTATAACTTGCAGATTCTGCTTCGAGAAGGCTGCAAGAAAATCCTCGTAGCTGACTCATTGTCGTTG
  CTGAAAAAAATGCACCGAACCCAAATGAAAGGCGTTCTGGTTGATGAAGAGAATATCTGTGAATCATGCC
  TTTCTCCCATCCTCCCAACAGATGCAGCGAAGCCCTTCAGCGTGGTGGTCTTCCACTGCCGGCACATGTT
  CCACAAGGAGTGCCTGCCTATGCCGAGCATGAATGCTCCTGCACAATACTGCAACATCTGTAGTGCTAAG
  AACCGTGGGCCTGGAAGTGCCATCCTGGAGATGAAGAAGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_172120
• Insert Size: 2562 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172120.4, NP_742118.3
• RefSeq Size: 3212 bp
• RefSeq ORF: 2562 bp
• Locus ID: 218035
• UniProt ID: Q5KU39
• Cytogenetics: 13 6.75 cM
• Gene Summary: Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act in part as a core component of the putative HOPS endosomal tethering complex is proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The HOPS complex is proposed to be recruited to Rab7 on the late endosomal membrane and to regulate late endocytic, phagocytic and autophagic traffic towards lysosomes. Involved in homotypic vesicle fusions between late endosomes and in heterotypic fusions between late endosomes and lysosomes implicated in degradation of endocytosed cargo. Required for fusion of autophagosomes with lysosomes. May link the HOPS complex to endosomal Rab7 via its association with RILP and to lysosomal membranes via its association with ARL8B, suggesting that these interactions may bring the compartments to close proximity for fusion. Involved in the direct trans-Golgi network to late endosomes transport of lysosomal membrane proteins independently of HOPS. Involved in sorting to the regulated secretory pathway presumably implicating the AP-3 adaptor complex. May play a role in HOPS-independent function in the regulated secretory pathway (By similarity).[UniProtKB/Swiss-Prot Function]