Efhb (NM_172497) Mouse Untagged Clone

CAT#: MC222072

Efhb (untagged) - Mouse EF hand domain family, member B (Efhb), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Efhb
• Synonyms: 4921525D22Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222072 representing NM_172497
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGTTCGTTTGTGAGAGTGGGGTCCCCCAAACCTTTGCAAACTTCAGCTTCTCCGTTAGAGATGAGTT
  CCCTGAGAAGGACGAGAGCACCTGAAATCTCGGAACTTGGACTCACCCCAGAGCAAAAGGACGATATAAG
  AGACAGGGTACTTAGGGGGTCAAAATCTCCCACGGAGCTGGGGTTTGACTTGAGACTAGAACAAGATCGA
  AAATGGAGAGAGAGGATGGGATCATCAGAAGCAAAATCGCCACCTTGCCATGCTCTTGGAGTAGGATTAG
  AAAGACATACTATTTCTGGGACACCCACAGAGATGGGTAATTTAGGACTTCACAAAGGCTCTGCCTTCCA
  GGGATCAAAACCTCTGGGTGTGTTACCAGGAAGAGTGGGGCCAGAAAACAAGGGCCTTCCGCCTCGCCTT
  CGCTACGGAGGGACACTACACCCTCCTTTCAGCACAGTACATGCAAGTCCGCTGGCGGCAGAGAGCAGAA
  GGAGGCCTTTAGCCTGGGGTTCTGCTTGGACGGATGCTGTAGTGGAAAAGCAACCTGTTGTAGGTTTGGA
  ACTGAGAAAAGAACCGGAAAAAGAACCCACCTGTGTCGTGATGAACCCCTACCCAGAGATGCCCCCTAAG
  GAGGTAGACATCGGGTTGCCACAAACTCAGGAATCAGATGAAGCCAAGAATACAGAGCCTCTAATAGGTT
  TAGTAAGAGAACCTTCTGAATGTCCATTTGCCCAACAACCGGAAGAGAAAAAGGAGCCCGGCAGCACCGA
  ACCCGGAGTGGAGCCTCCAGGGAACATCAGGCCCATATACTCAGGGAAGTTCTTTGATCGGGTGCCTTGC
  TGGCCAAGTGCAGGAAAAGTTAAGCCAGTTGGATACAGAGTTGCAACCTGTTTGACTGAGAAGCTTCCCA
  GGCTAATGACTCCCCCAGAGGCAAAGAAGTATTTCAACTTCAGATATCCACCTGCTGGAGCAGAAAGAGT
  ATTTTATGGCAGAGCAAATGACCCCCAAATTGCACCTTATTTGACACATGGACTAAGATCTAAAATCTCA
  ATACCGATGGGATCATTGATAAACCCACAGCCCATTACCACGTTTCAACAGAAAATTAAAGACAAAAAAG
  AATCTATATATTTTAGCCATCAACGAGCACCATTGGGAAAATCTCATGATCAAACACCAGGATTGCCAAA
  AGGAATGGATGTGATCAACACAACATTAGGGACACCGACTATCAGAGAACTGTCTGTTCGTGACACTGTG
  AATCCATCGAAATCCTTCGAAGATGTGCTTAAAGAAGGACAGGAAGGACATGATTTGTACACTGTCTCTC
  ACAATGATTATTTTGCAGGAGAAGCAAAGAATCGAAAATATAACCCGGCAAGCTTCCATAGGTTTAACTT
  GTATGGAATCCCAACACCTCACTTTAATGATGGGCGCACCATGGCTAAAGCTTTACATTGGCTCCATGAA
  CTACAAATGGAAAGAGGAGCGAAAATTGTATCCAAAAGAGTTGATGACTTCAAAGAGAAATTTCAACACA
  AACTTGGAAAAGTTTTAGACCCCATTGCAGAAACTATGAATGTTCCCCCAGGTCACACATTTGGAAGTTG
  CCTCCATCCTGAGGAATATGGAGCTGGCGATCTCATCCATTATAGAAGCCCAGATGAATATCTTCGTGGG
  AAGGACCACCAGAGAGCCGTGGTTGCAGCTGCTCGCCACCACTTGAAGAAATTTAATCACCAGAACTTTG
  ACACTTTGCAGGTGGCTTTCAGGCACTATGACAAGAAGGGAGATGGGGTGATTGACAGAGCAGAACTTCA
  TGAAGCTTGTGTCCAAGCCAACCTGCATCTAGACAAGATGCTCCTGGACCATCTGTTTGACTACTGTGAC
  GTGGACCAGGATGGCTTGATCAACTACCTGGAATTTGCCAATTTCCTTAACTGGAAAGACAGAATACCTC
  TCAAAGAGCATGAGAAGAGGGTCGTTGTTAAAGGTAAAAAACCAGACTGTGAAAACGTTACTGATACTAG
  CATGGGAGAAGCTGAACCAAGTCTCCTGATAAATCCTGAAGACATTGTCCCCAAAGAACCAGGAAGTTCA
  GAAGAGACTCTCCGGACAATCCAGAGACCAGGTGACAAAGTTTCTCACCAGTATAAGACAACGTCTTCTG
  AGATCAACGCTGTGGTTGGAGCTGTCCCCTCCATGTGTCACCCCATATTCGGTGTTCCAACCATTCGATC
  TGACATCTCTGCTCCCCGGATTCGTCGAGTCAGTGACATGAATAACTACGGTGACGAAGGCAATGCTTAC
  TCTCTGCTGCACCCCTCCATCTTCTCCCAGAAAGGTGTATTTGAGAGAGACTTCTTCAAGACAAGGTCAA
  AGGAAGAGATTTCAGATATATTAACTAACATTGGAGTCAAGCTTTCTAAGGAAGAATTCGAAAATGTGTG
  GAATCTTGCTTCAAAAAAACACCAGCGAGGAGAAGTTTGTGTTGAAACTATCAGGAACGTTCTAGATGAG
  CTACTGCATGCAGACCTCGTCAAGTGTAAAACGGCCATGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_172497
• Insert Size: 2562 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172497.3, NP_766085.2
• RefSeq Size: 2661 bp
• RefSeq ORF: 2562 bp
• Locus ID: 211482
• UniProt ID: Q8CDU5
• Cytogenetics: 17 C
• Gene Summary: Cytosolic sensor for calcium, modulates the interaction of STIM1 and ORAI1 upon store depletion, the activation of store-operated Ca(2+) entry (SOCE) and NFAT translocation from cytosol to nucleus.[UniProtKB/Swiss-Prot Function]