Arhgap17 (NM_144529) Mouse Untagged Clone

CAT#: MC222061

Arhgap17 (untagged) - Mouse Rho GTPase activating protein 17 (Arhgap17), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Arhgap17
• Synonyms: 5730403H17Rik; Nadrin; Nadrin2; Rich1; Wbp15
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222061 representing NM_144529
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGAAGCAATTCAACCGCATGAAGCAGCTGGCCAATCAGACTGTCGGCAGAGCTGAGAAGACCGAAG
  TCCTCAGTGAAGATCTGCTACAGATTGAACGTCGCCTGGACACTGTGCGTTCAATGTGCCACCATTCACA
  TAAGCGTTTGATAGCCTGCTTCCAAGGTCAGCATGGCACTGATGCCGAGAGGAGACATAAAAAGCTTCCT
  CTGACAGCTCTTGCCCAGAACATGCAGGAGGCTTCAGCCCAGCTGGAAGAGTCTCTCTTGGGGAAGATGC
  TGGAGACCTGTGGGGACGCTGAGAACCAGCTGGCTCTTGAACTCTCACAACATGAAGTCTTTGTGGAGAA
  GGAGATCATGGACCCTCTCTACGGCATAGCAGAGGTGGAGATTCCCAATATCCAGAAGCAAAGGAAGCAG
  CTTGCTAGATTGGTGTTGGACTGGGATTCAGTCAGAGCGAGATGGAACCAAGCACACAAGTCTTCAGGAA
  CCAACTTTCAGGGGCTTCCATCCAAAATAGATACCCTAAAGGAAGAGATGGATGAAGCTGGGAATAAAGT
  TGAACAGTGCAAGGATCAACTTGCAGCAGACATGTACAACTTCATGGCCAAAGAAGGGGAGTATGGCAAG
  TTCTTCGTGACGTTATTAGAAGCCCAAGCAGATTACCATAGAAAAGCATTAGCAGTCTTAGAAAAGGCCC
  TTCCCGAAATGCGCGCCCATCAAGATAAGTGGGCAGAGAAGCCAGCCTTCGGCACACCTCTGGAGGAACA
  CCTGAAGAGGAGTGGGCGTGAGATCGCCCTGCCTATTGAGGCCTGTGTCATGTTGTTGCTGGAGACTGGC
  ATGAAAGAGGAGGGCCTTTTTCGGATTGGAGCTGGAGCCTCCAAGTTGAAGAAGCTGAAAGCTGCTCTCG
  ACTGCTCCACATCGCATCTGGATGAATTCTATTCTGATCCCCATGCTGTCGCAGGTGCTTTAAAGTCCTA
  TCTGCGGGAGTTGCCTGAGCCCTTAATGACTTTCAGTCTGTATGAAGAATGGACACAAGTTGCAAGTGTG
  CAGGATCAAGACAAAAAACTTCAATATTTATGGACAACATGTCAGAAGTTGCCGCCACAAAATTTTGTTA
  ACTTTAGGTATTTAATCAAGTTCCTTGCAAAGCTTGCCCAGACCAGTGACGTTAATAAAATGACTCCTAG
  CAACATAGCCATTGTCCTGGGCCCTAACCTCCTGTGGGCCAAACAAGAAGGAACGCTGGCTGAAATAGCG
  GCTGCCACATCGGTCCACGTGGTTGCAGTGATTGAGCCCATCATCCAGCATGCAGATTGGTTCTTCCCTG
  GAGAGGTAGAATTCAATGTATCAGAAGCATTTGTGCCACTTGCTACCCCGAATTCTAATCACTCATCCCA
  TACTGGAAATGACTCTGACTCGGGGACTCTGGAGAGGAAGCGACCTGCCAGCATGGCAGTGATGGAAGGG
  GACTTGGTGAAGAAGGAGAGCTTTGGTGTGAAGCTAATGGACTTCCAGGCCCACCGGCGGGGTGGCACTC
  TAAATAGAAAGCACATAGCCCCCGCTTTCCAGCCGCCACTCCCGCCCACGGATGGCAACGCCTTGGCTCC
  AGCAGGCCCAGAGCCCCCTTCTCAGAGCTCTAGGGCTGACAGCAGCTCAGGGGGTGGGCCTGTCTTCTCT
  TCTACGGGCATATTGGAGCAGGGGCTGAGCCCAGGTGACAGCAGTCCTCCAAAACCCAAAGACTCCGTGT
  CTGCAGCTGTCCCTGCAGCAGGAAGGAACAGCAACCAGATGACCACGGTCCCAAACCAGGCTCAGACAGG
  TGGCAACTCCCATCAGCTCTCAGTAAGCACACCTCACAGTGCAGCCGGTCCCAGCCCGCACACTCTGCGT
  CGGGCTGTGAAGAAACCTGCCCCCGCACCCCCCAAACCAGGAAACCTACCTCCTGGCCACCCTGGAGGAC
  AGAGCTCTCCTGGCACAGGCACATCCCCAAAGCCAAGCGCCCGAAGCCCATCACCGCCTCAGCAGCAGCA
  GCAGCAGCAACAACAGCAGCAACAACAGCAGCAGCAGCAGACCCCAGGCATGCGCCGCTGCTCCAGCAGC
  CTGCCTCCCATCCAGGCACCCAGCCACCCACCACCACAGCCCCCCACACAGCCTCGGTTGGGTGAACAGG
  GACCAGAGCCAGGCCCCACGCCACCTCAAACCCCCACACCACCTAGCACCCCACCCCTGGCCAAGCAGAA
  TCCATCGCAAAGTGAGACCACACAGCTGCACGGAACCCTCCCAAGACCACGGCCAGTGCCCAAGCCCCGC
  AACCGGCCTAGCGTGCCACCACCCCCACATCCACCTGGCACCCACACGGTGGATGGTGGCCTTACATCCT
  CAGTGCCCACAGCCTCCAGAATCGTCACTGACACCAATTCTAGGGTTTCTGAATCACTTCGCAGCATCTT
  TCCTGAAATACATTCAGACTTGGCAAGCAAAGAAGTGCCTGGCCACATCCTGCTGGACATAGACAATGAC
  ACAGAAAGTACTGCATTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_144529
• Insert Size: 2541 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144529.2, NP_653112.2
• RefSeq Size: 3503 bp
• RefSeq ORF: 2541 bp
• Locus ID: 70497
• UniProt ID: Q3UIA2
• Cytogenetics: 7 F3
• Gene Summary: Rho GTPase-activating protein involved in the maintenance of tight junction by regulating the activity of CDC42, thereby playing a central role in apical polarity of epithelial cells. Specifically acts as a GTPase activator for the CDC42 GTPase by converting it to an inactive GDP-bound state. The complex formed with AMOT acts by regulating the uptake of polarity proteins at tight junctions, possibly by deciding whether tight junction transmembrane proteins are recycled back to the plasma membrane or sent elsewhere. Participates in the Ca(2+)-dependent regulation of exocytosis, possibly by catalyzing GTPase activity of Rho family proteins and by inducing the reorganization of the cortical actin filaments. Acts as a GTPase activator in vitro for RAC1 (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longest protein (isoform a).