Atg9a (NM_001003917) Mouse Untagged Clone

CAT#: MC222029

Atg9a (untagged) - Mouse autophagy-related 9A (yeast) (Atg9a), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Atg9a
• Synonyms: Apg9l1; Atg9; Atg9l1; AU019532
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222029 representing NM_001003917
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCACAGTTTGACACTGAATACCAGCGCCTAGAGGCCTCCTATAGCGATTCGCCCCCCGGGGAAGAGG
  ACCTGTTGGTGCATGTGGCCGAGGGGAGCAAATCACCTTGGCACCACATTGAAAACCTTGACCTCTTCTT
  CTCTCGAGTTTATAATCTCCACCAGAAGAATGGCTTCACATGTATGCTCATTGGAGAGATGTTTGAACTC
  ATGCAGTTCCTCTTTGTGGTTGCCTTCACCACCTTCCTGGTTAGCTGTGTGGACTATGACATCCTCTTTG
  CCAACAAGATGGTGAACCACAGTCTCCATCCTACTGAGCCTGTCAAGGTCACTCTGCCAGATGCCTTTTT
  GCCTGCCCAAGTCTGTAGTGCCAGGATTCAGGAAAATGGCTCTCTTATCACCATCCTGGTCATTGCTGGT
  GTCTTCTGGATCCACCGGCTTATCAAGTTTATCTATAACATTTGCTGCTATTGGGAGATCCACTCCTTCT
  ACCTACATGCTCTTCGGATCCCAATGTCTGCCCTTCCATACTGCACATGGCAGGAAGTTCAGGCCCGGAT
  TGTGCAGACGCAGAAAGAGCATCAGATCTGCATCCACAAGCGTGAGCTGACAGAGTTGGACATCTACCAT
  CGCATCCTACGTTTCCAGAACTACATGGTGGCCCTGGTGAACAAATCCCTCCTGCCTCTGCGCTTCCGTC
  TGCCCGGCCTCGGAGAGGTTGTCTTCTTCACCCGTGGCCTCAAGTACAACTTTGAGCTCATCCTCTTCTG
  GGGACCCGGCTCTCTGTTTCTCAATGAGTGGAGCCTCAAGGCCGAGTACAAACGTGGAGGGCAACGGCTA
  GAGCTGGCCCAGCGTCTCAGCAACCGCATCTTGTGGATTGGCATCGCCAACTTCCTGCTGTGTCCCCTCA
  TCCTCATCTGGCAGATCCTCTATGCCTTCTTCAGCTATGCCGAGGTGCTGAAGAGAGAGCCGGGGGCCCT
  GGGAGCGCGTTGCTGGTCACTCTATGGCCGTTGTTACCTCCGCCACTTCAATGAGCTGGAGCATGAGCTG
  CAGTCCCGCCTCAACCGAGGCTACAAGCCCGCCTCCAAGTACATGAATTGCTTCTTGTCACCGCTGCTGA
  CTCTGCTGGCCAAGAATGGTGCCTTCTTCGCTGGCTCTATCCTGGCTGTGCTTATTGCCCTCACCATCTA
  TGATGAAGATGTGTTAGCTGTGGAACACGTCCTCACCACGGTCACCCTCCTGGGAGTCACGGTGACTGTG
  TGCAGGTCCTTCATCCCAGACCAGCACATGGTGTTCTGCCCCGAGCAGCTGCTCCGAGTGATTCTTGCAC
  ACATCCACTACATGCCTGACCACTGGCAGGGTAATGCCCACCGCTCGCAGACCCGGGACGAGTTTGCCCA
  GCTCTTCCAGTACAAGGCAGTGTTCATCTTGGAGGAGTTGCTGAGTCCCATCGTCACACCCCTCATTCTC
  ATCTTCTGCCTCCGCCCTCGGGCCCTGGAGATCATAGACTTCTTCCGCAACTTTACGGTCGAGGTCGTGG
  GTGTGGGAGACACCTGCTCCTTTGCTCAGATGGACGTTCGCCAGCATGGCCATCCTCAGTGGCTGTCTGG
  AGGGCAGACAGAGGCCTCAGTGTACCAGCAAGCCGAGGACGGGAAGACCGAGTTGTCGCTCATGCACTTT
  GCCATCACCAATCCCGGCTGGCAGCCCCCTCGTGAGAGCACAGCTTTCCTGGGCTTCCTCAAGGAGCAGG
  TGCAGCGAGATGGAGCAGCTGCTGGCCTGGCCCAGGGTGGCCTGCTCCCCGAGAATGCCCTCTTCACATC
  CATCCAGTCCTTACAGTCTGAGTCCGAGCCACTGAGCCTTATTGCAAATGTGGTAGCAGGCTCATCCTGC
  CGAGGACCCTCACTGTCCAGAGACCTGCAGGGCTCCAGGCACAGGGCTGATGTTGCTTCTGCCCTTCGAT
  CCTTCTCCCCTCTGCAGCCTGGAGCGGCCCCTCAAGGCCGGGTTCCCAGTACCATGACAGGCTCTGGAGT
  GGATGCCAGGACAGCCAGCTCTGGGAGTAGCGTGTGGGAAGGACAGCTGCAGAGCCTGGTGCTGTCCGAA
  TACGCGTCCACCGAGATGAGCCTGCACGCCCTGTACATGCACCAGCTCCACAAGCAGCAGACCCAGGCTG
  AGCCCGAGCGGCATGTGTGGCACCGCCGGGAGAGTGATGAGAGTGGAGAGAGTGCCCCTGAAGAGGGGGG
  AGAGGGTGCCCGGGCCCCCCAACCCATCCCCCGCTCGGCCAGCTATCCCTGTGCTACACCCCGGCCTGGA
  GCACCTGAGACCACCGCCCTGCATGGGGGCTTCCAGAGGCGCTACGGGGGCATCACAGATCCTGGCACAG
  TGCCCCGTGGCCCCTCTCACTTCTCCAGGCTGCCCCTGGGAGGATGGGCAGAAGATGGCCAGCCAGCATC
  AAGGCACCCAGAGCCGGTGCCAGAGGAGGGCTCAGAGGATGAACTCCCCCCTCAAGTGCACAAGGTATAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001003917
• Insert Size: 2520 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001003917.4, NP_001003917.2
• RefSeq Size: 3856 bp
• RefSeq ORF: 2520 bp
• Locus ID: 245860
• UniProt ID: Q68FE2
• Cytogenetics: 1 C4
• Gene Summary: Involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS), the nucleating site for formation of the sequestering vesicle. Cycles between a juxta-nuclear trans-Golgi network compartment and late endosomes. Nutrient starvation induces accumulation on autophagosomes. Starvation-dependent trafficking requires ULK1, ATG13 and SUPT20H (By similarity). Required for carbonyl cyanide m-chlorophenylhydrazone (CCCP)-induced ATG8 family proteins lipidation, a key autophagy step.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same isoform (a).