Pygb (NM_153781) Mouse Untagged Clone

CAT#: MC222017

Pygb (untagged) - Mouse brain glycogen phosphorylase (Pygb), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pygb
• Synonyms: MGC36329
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222017 representing NM_153781
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGAAGCCGTTGACGGACAGTGAGAGGCAGAAGCAGATCAGCGTGCGTGGCATCGCGGGGCTGGGCG
  ACGTGGCCGAGGTGCGGAAGAGCTTCAACAGACACCTGCATTTCACGCTGGTCAAGGACCGCAATGTGGC
  CACGCCCCGCGACTACTTCTTCGCTCTGGCACACACAGTTCGCGACCACCTAGTGGGCCGCTGGATCCGC
  ACGCAGCAGCATTACTATGAGCGGGACCCCAAGCGCATCTATTACCTTTCCCTGGAATTCTACATGGGCC
  GCACGCTGCAGAATACCATGGTGAACCTGGGCCTTCAGACTGCATGCGACGAAGCCACTTATCAGTTGGG
  GTTGGACTTGGAAGAGCTAGAGGAGATAGAGGAGGATGCTGGTCTTGGGAATGGCGGCCTGGGGCGCCTG
  GCAGCCTGCTTCCTTGATTCAATGGCCACCCTGGGTCTGGCTGCATACGGCTATGGAATCCGATATGAAT
  TTGGGATTTTCAACCAAAAGATTGTCAATGGCTGGCAGGTTGAAGAAGCTGATGACTGGCTACGCTATGG
  AAACCCCTGGGAGAAGGCACGGCCGGAGTACATGCTGCCGGTGCATTTCTACGGGAGGGTGGAGCACACC
  CCTGACGGGGTCCTCTGGCTGGACACGCAGGTGGTACTCGCTATGCCCTACGACACCCCAGTGCCTGGGT
  ATAAGAACAACACAGTCAACACCATGAGGCTCTGGTCTGCCAAGGCACCCAATGACTTCAAGCTGAAGGA
  CTTCAACGTTGGGGACTACATCGAAGCTGTCCTGGATAGGAACTTGGCTGAGAACATCTCCAGGGTCCTG
  TATCCCAATGACAATTTCTTCGAGGGGAAGGAGCTGCGGCTGAAGCAGGAGTACTTTGTGGTGGCTGCCA
  CCCTCCAGGACATCATTCGAAGGTTCAAGTCTTCCAGGTTTGGCTGCCGGGACCCAGTGAGAACCTGTTT
  CGAGACATTCCCAGACAAGGTAGCCATCCAGCTGAATGACACCCACCCCGCGCTCTCCATCCCTGAGCTC
  ATGCGGATCCTGGTGGACGTGGAGAAGGTGGACTGGGATAAGGCCTGGGAGATCACCAAGAAGACCTGTG
  CCTACACCAACCACACGGTGCTGCCCGAGGCCCTGGAGCGCTGGCCAGTGTCTATGTTTGAGAAGCTGCT
  TCCGCGGCACCTGGAAATAATCTATGCCATCAACCAGAGGCACTTGGACCACGTGGCTGCCCTGTTTCCT
  GGGGACGTGGACCGACTGAGGAGGATGTCCGTGATTGAGGAAGGGGACTGCAAGAGAATCAACATGGCAC
  ACCTGTGTGTGATTGGGTCCCACGCTGTGAACGGGGTGGCAAGGATTCACTCTGAGATCGTGAAGCAGTC
  TGTCTTTAAGGACTTTTACGAACTGGAGCCAGAGAAGTTCCAGAACAAGACAAATGGCATCACGCCCCGA
  CGGTGGCTGCTGTTGTGTAACCCGGGGCTGGCAGAAATCATTGTAGAGAGAATCGGGGAAGGTTTCCTGA
  CCGATCTGAGCCAACTGAAGAAGCTGCTGTCACTGGTTGATGATGAGGCCTTCATCAGAGACGTAGCCAA
  GGTTAAGCAGGAAAACAAGCTGAAGTTCTCTGCCCAGCTAGAGAAGGAGTACAAGGTGAAGATCAACCCT
  GCATCCATGTTTGATGTGCACGTGAAGAGGATCCACGAATATAAGCGACAGCTGCTCAACTGCCTACACA
  TTATCACCCTGTACAACCGAATAAAGAAGGATCCAGCCAAGGCCTTTGTGCCCAGGACTGTTATGATTGG
  GGGCAAGGCAGCTCCTGGTTACCACATGGCTAAGATGATCATCAAGCTGGTCACATCCATTGGAGATGTT
  GTCAATCATGATCCAGTTGTGGGCGACAGGCTCAGAGTGATCTTCCTGGAGAACTACCGTGTGTCTTTAG
  CTGAGAAAGTGATCCCAGCGGCTGACCTGTCCCAGCAGATCTCCACTGCGGGCACTGAAGCCTCGGGCAC
  AGGCAACATGAAGTTCATGCTCAATGGAGCGCTCACCATCGGCACCATGGATGGTGCCAACGTGGAGATG
  GCCGAGGAGGCCGGGGAGGAGAACCTCTTCATCTTTGGTATGCGGGTGGAAGATGTCGAGGCCCTGGACC
  AGAAAGGGTACAATGCCAGAGAATTCTATGAGCGCCTGCCTGAGCTGAGGCAGGCTGTGGATCAGATCAG
  CAGTGGCTTCTTTTCTCCCAAAGATCCAGACTGCTTCAAGGACGTGGTTAACATGCTGATGTACCACGAC
  AGATTCAAGGTATTTGCAGACTATGAAGCCTACATCCAATGCCAGGCCCAGGTGGACCGTCTATACCGGA
  ATTCCAAGGAGTGGACTAAAAAGGTGATCAGGAATATAGCCTGCTCTGGCAAGTTCTCCAGTGACCGGAC
  CATCACTGAATACGCCCGGGAGATCTGGGGTGTGGAGCCCTCTGACCTGCAGATCCCACCCCCCAACCTC
  CCCAAGGACTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_153781
• Insert Size: 2532 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153781.1, NP_722476.1
• RefSeq Size: 3860 bp
• RefSeq ORF: 2532 bp
• Locus ID: 110078
• UniProt ID: Q8CI94
• Cytogenetics: 2 74.74 cM
• Gene Summary: Glycogen phosphorylase that regulates glycogen mobilization. Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties.[UniProtKB/Swiss-Prot Function]