Rbm25 (NM_027349) Mouse Untagged Clone

CAT#: MC222005

Rbm25 (untagged) - Mouse RNA binding motif protein 25 (Rbm25), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rbm25
• Synonyms: 2600011C06Rik; 2610015J01Rik; A130095G20Rik; AI159652; AL023075; AU043498; RNPC7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222005 representing NM_027349
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTTTCCCCCCTCATTTGAATCGCCCTCCCATGGGAATCCCAGCTCTCCCTCCAGGGATCCCACCCC
  CACAGTTTCCTGGCTTTCCTCCACCAGTACCTCCAGGGACACCGATGATTCCTGTACCAATGAGTATTAT
  GGCTCCTGCTCCAACTGTCCTAGTACCTACCGTGTCCATGGTTGGAAAGCATTTGGGAGCCAGAAAGGAT
  CATCCAGGCTTAAAATTAAAAGAAAATGATGAAAATTGTGGCCCTACTACTACAGTTTTTGTTGGGAACA
  TTTCTGAGAAGGCTTCAGACATGCTTATACGACAGCTCTTAGCTAAATGTGGTTTGGTGTTGAGCTGGAA
  GAGAGTGCAAGGTGCTTCTGGGAAACTTCAAGCTTTTGGATTTTGTGAGTATAAAGAGCCTGAATCTACC
  CTCCGTGCTCTCAGATTATTGCATGACCTTCAGATTGGAGAGAAGAAGCTACTTGTTAAAGTTGATGCCA
  AGACAAAGGCACAGTTGGATGAATGGAAAGCAAAGAAGAAAGCTAATGGGAACGCCAGACCAGAAACTGT
  CACTAATGATGATGAAGAAGCCTTAGATGAAGAAACAAAGCGAAGAGACCAGATGATTAAAGGGGCCATT
  GAAGTTTTAATCCGAGAATATTCCAGTGAACTAAATGCCCCCTCACAGGAGTCTGACTCTCACCCCAGAA
  AGAAGAAAAAGGAAAAGAAGGAGGACATTTTCCGTAGATTTCCAGTGGCCCCATTGATCCCTTACCCACT
  CATCACCAAGGAGGATATAAATGCTATTGAAATGGAAGAAGACAAAAGAGATTTGATATCCCGAGAGATC
  AGCAAATTCAGAGATACACACAAGAAACTGGAAGAAGAGAAAGGCAAAAAAGAAAAAGAAAGACAGGAAA
  TTGAGAAAGAACGGAGAGAAAGAGAGAGGGAACGTGAAAGGGAACGAGAAAGGCGAGAACGGGAACGAGA
  AAGGGAAAGAGAACGTGAACGAGAAAAGGAAAAAGAACGGGAGCGGGAACGAGAACGGGATAGGGATCGT
  GACCGCACAAAAGAGAGAGACCGAGATCGAGAGAGAGATCGGGACCGAGATCGAGAACGGAGCTCAGATC
  GAAATAAGGATCGGAGTCGATCAAGAGAAAAAAGCAGAGATCGTGAAAGGGAACGGGAGCGGGAAAGAGA
  GAGAGAACGAGAACGGGAACGAGAACGAGAGCGAGAGAGAGAACGGGAGAGAGAACGGGAACGGGAAAGA
  GAGAAAGACAAGAAAAGAGACAGAGAAGAGGATGAAGAAGATGCATATGAACGAAGAAAACTCGAACGAA
  AACTGCGAGAGAAAGAGGCTGCTTATCAAGAGCGCCTTAAGAATTGGGAAATCAGAGAACGAAAGAAAAC
  TAGGGAATATGAGAAGGAGGCAGAAAGAGAAGAAGAAAGAAGAAGAGAAATGGCTAAGGAGGCTAAACGA
  CTAAAAGAATTCCTAGAAGATTATGATGATGATAGAGATGACCCGAAGTACTACAGGGGTAGTGCTCTTC
  AGAAACGGTTGCGTGATAGGGAAAAAGAAATGGAAGCTGATGAACGAGACAGGAAGAGAGAGAAGGAAGA
  GCTTGAGGAAATCAGGCAGCGCTTACTGGCAGAAGGGCATCCAGATCCAGATGCAGAGCTCCAGAGGATG
  GAACAAGAGGCTGAGAGGCGCAGGCAACCTCAAATAAAGCAAGAACCAGAATCCGAGGAAGAAGAAGAAG
  AAAAGCAAGAAAAAGAAGAAAAACGAGAGGAGCCTGTGGAAGAGGAGGAAGAACCAGAGCAAAAGCCTTG
  TCTCAAGCCGACTTTGAGACCCATCAGCTCTGCCCCATCTGTGTCCTCTGCCAGTGGCAACGCAACCCCC
  AACACTCCTGGGGATGAGTCTCCTTGTGGTATTATTATTCCTCATGAAAACTCACCAGATCAACAGCAGC
  CTGAGGAGCATAGGCCAAAAATAGGACTAAGCCTTAAATTGGGTGCTTCCAATAGTCCTGGCCAACCTAA
  TTCAGTGAAGAGAAAGAAACTACCTGTAGATAGTGTCTTTAACAAATTCGAGGATGAGGACAGTGATGAT
  GTGCCCCGAAAAAGGAAACTCGTTCCCCTGGATTATGGCGAAGATGATAAAAATGCCACCAAAGGCACTG
  TAAACACTGAAGAAAAGCGCAAACACATTAAGAGTCTCATTGAGAAAATTCCCACAGCCAAACCTGAGCT
  CTTTGCTTATCCTTTGGATTGGTCTATTGTGGATTCTATACTGATGGAACGAAGAATCCGGCCATGGATC
  AATAAGAAAATCATCGAATACATAGGTGAAGAAGAAGCTACATTAGTTGATTTTGTTTGCTCTAAGGTTA
  TGGCACATAGTTCACCACAGAGCATTTTAGATGATGTTGCCATGGTACTAGATGAAGAAGCAGAAGTTTT
  TATAGTCAAAATGTGGAGATTATTGATATATGAAACAGAAGCCAAGAAAATTGGCCTTGTGAAGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_027349
• Insert Size: 2517 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027349.3, NP_081625.3
• RefSeq Size: 4244 bp
• RefSeq ORF: 2517 bp
• Locus ID: 67039
• UniProt ID: B2RY56
• Cytogenetics: 12 D1
• Gene Summary: RNA-binding protein that acts as a regulator of alternative pre-mRNA splicing. Involved in apoptotic cell death through the regulation of the apoptotic factor BCL2L1 isoform expression. Modulates the ratio of proapoptotic BCL2L1 isoform S to antiapoptotic BCL2L1 isoform L mRNA expression. When overexpressed, stimulates proapoptotic BCL2L1 isoform S 5'-splice site (5'-ss) selection, whereas its depletion caused the accumulation of antiapoptotic BCL2L1 isoform L. Promotes BCL2L1 isoform S 5'-ss usage through the 5'-CGGGCA-3' RNA sequence. Its association with LUC7L3 promotes U1 snRNP binding to a weak 5' ss in a 5'-CGGGCA-3'-dependent manner. Binds to the exonic splicing enhancer 5'-CGGGCA-3' RNA sequence located within exon 2 of the BCL2L1 pre-mRNA (By similarity).[UniProtKB/Swiss-Prot Function]