Dnmt3b (NM_001003960) Mouse Untagged Clone

CAT#: MC221993

Dnmt3b (untagged) - Mouse DNA methyltransferase 3B (Dnmt3b), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dnmt3b
• Synonyms: MmuIIIB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221993 representing NM_001003960
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGGAGACAGCAGACATCTGAATGAAGAAGAGGGTGCCAGCGGGTATGAGGAGTGCATTATCGTTA
  ATGGGAACTTCAGTGACCAGTCCTCAGACACGAAGGATGCTCCCTCACCCCCAGTCTTGGAGGCAATCTG
  CACAGAGCCAGTCTGCACACCAGAGACCAGAGGCCGCAGGTCAAGCTCCCGGCTGTCTAAGAGGGAGGTC
  TCCAGCCTTCTGAATTACACGCAGGACATGACAGGAGATGGAGACAGAGATGATGAAGTAGATGATGGGA
  ATGGCTCTGATATTCTAATGCCAAAGCTCACCCGTGAGACCAAGGACACCAGGACGCGCTCTGAAAGCCC
  GGCTGTCCGAACCCGACATAGCAATGGGACCTCCAGCTTGGAGAGGCAAAGAGCCTCCCCCAGAATCACC
  CGAGGTCGGCAGGGCCGCCACCATGTGCAGGAGTACCCTGTGGAGTTTCCGGCTACCAGGTCTCGGAGAC
  GTCGAGCATCATCTTCAGCAAGCACGCCATGGTCATCCCCTGCCAGCGTCGACTTCATGGAAGAAGTGAC
  ACCTAAGAGCGTCAGTACCCCATCAGTTGACTTGAGCCAGGATGGAGATCAGGAGGGTATGGATACCACA
  CAGGTGGATGCAGAGAGCAGAGATGGAGACAGCACAGAGTATCAGGATGATAAAGAGTTTGGAATAGGTG
  ACCTCGTGTGGGGAAAGATCAAGGGCTTCTCCTGGTGGCCTGCCATGGTGGTGTCCTGGAAAGCCACCTC
  CAAGCGACAGGCCATGCCCGGAATGCGCTGGGTACAGTGGTTTGGTGATGGCAAGTTTTCTGAGATCTCT
  GCTGACAAACTGGTGGCTCTGGGGCTGTTCAGCCAGCACTTTAATCTGGCTACCTTCAATAAGCTGGTTT
  CTTATAGGAAGGCCATGTACCACACTCTGGAGAAAGCCAGGGTTCGAGCTGGCAAGACCTTCTCCAGCAG
  TCCTGGAGAGTCACTGGAGGACCAGCTGAAGCCCATGCTGGAGTGGGCCCACGGTGGCTTCAAGCCTACT
  GGGATCGAGGGCCTCAAACCCAACAAGAAGCAACCAGAGAACAAAAGTCGAAGACGCACAACCAATGACT
  CTGCTGCTTCTGAGTCCCCCCCACCCAAGCGCCTCAAGACAAATAGCTATGGCGGGAAGGACCGAGGGGA
  GGATGAGGAGAGCCGAGAACGGATGGCTTCTGAAGTCACCAACAACAAGGGCAATCTGGAAGACCGCTGT
  TTGTCCTGTGGAAAGAAGAACCCTGTGTCCTTCCACCCCCTCTTTGAGGGTGGGCTCTGTCAGAGTTGCC
  GGGATCGCTTCCTAGAGCTCTTCTACATGTATGATGAGGACGGCTATCAGTCCTACTGCACCGTGTGCTG
  TGAGGGCCGTGAACTGCTGCTGTGCAGTAACACAAGCTGCTGCAGATGCTTCTGTGTGGAGTGTCTGGAG
  GTGCTGGTGGGCGCAGGCACAGCTGAGGATGCCAAGCTGCAGGAACCCTGGAGCTGCTATATGTGCCTCC
  CTCAGCGCTGCCATGGGGTCCTCCGACGCAGGAAAGATTGGAACATGCGCCTGCAAGACTTCTTCACTAC
  TGATCCTGACCTGGAAGAATTTGAGCCACCCAAGTTGTACCCAGCAATTCCTGCAGCCAAAAGGAGGCCC
  ATTAGAGTCCTGTCTCTGTTTGATGGAATTGCAACGGGGTACTTGGTGCTCAAGGAGTTGGGTATTAAAG
  TGGAAAAGTACATTGCCTCCGAAGTCTGTGCAGAGTCCATCGCTGTGGGAACTGTTAAGCATGAAGGCCA
  GATCAAATATGTCAATGACGTCCGGAAAATCACCAAGAAAAATATTGAAGAGTGGGGCCCGTTCGACTTG
  GTGATTGGTGGAAGCCCATGCAATGATCTCTCTAACGTCAATCCTGCCCGCAAAGGTTTATATGAGGGCA
  CAGGAAGGCTCTTCTTCGAGTTTTACCACTTGCTGAATTATACCCGCCCCAAGGAGGGCGACAACCGTCC
  ATTCTTCTGGATGTTCGAGAATGTTGTGGCCATGAAAGTGAATGACAAGAAAGACATCTCAAGATTCCTG
  GCATGTAACCCAGTGATGATCGATGCCATCAAGGTGTCTGCTGCTCACAGGGCCCGGTACTTCTGGGGTA
  ACCTACCCGGAATGAACAGGCCCGTGATGGCTTCAAAGAATGATAAGCTCGAGCTGCAGGACTGCCTGGA
  GTTCAGTAGGACAGCAAAGTTAAAGAAAGTGCAGACAATAACCACCAAGTCGAACTCCATCAGACAGGGC
  AAAAACCAGCTTTTCCCTGTAGTCATGAATGGCAAGGACGACGTTTTGTGGTGCACTGAGCTCGAAAGGA
  TCTTCGGCTTCCCTGCTCACTACACGGACGTGTCCAACATGGGCCGCGGCGCCCGTCAGAAGCTGCTGGG
  CAGGTCCTGGAGTGTACCGGTCATCAGACACCTGTTTGCCCCCTTGAAGGACTACTTTGCCTGTGAATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001003960
• Insert Size: 2520 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001003960.4, NP_001003960.2
• RefSeq Size: 4281 bp
• RefSeq ORF: 2520 bp
• Locus ID: 13436
• UniProt ID: O88509
• Cytogenetics: 2 H1
• Gene Summary: This is one of two related genes encoding de novo DNA methyltransferases, which are responsible for the establishment of DNA methylation patterns in embryos. Loss of function of this gene results in severe developmental defects and loss of viability. Mutation of the related gene in humans causes immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. There is a pseudogene for this gene located adjacent to this gene in the same region of chromosome 2. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Nov 2012]
  Transcript Variant: This variant (2) differs in the 5' UTR, and contains multiple differences in the coding region, but maintains the reading frame, compared to variant 5. The encoded isoform (2, also known as Dnmt3b2) is shorter than isoform 5. Variants 2 and 7 encode the same isoform (2).