Atp6v0a1 (NM_016920) Mouse Untagged Clone

CAT#: MC221989

Atp6v0a1 (untagged) - Mouse ATPase, H+ transporting, lysosomal V0 subunit A1 (Atp6v0a1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Atp6v0a1
• Synonyms: AA959968; ATP6a1; Atp6n1; Atp6n1a; Atpv0a1; Vpp-1; Vpp1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221989 representing NM_016920
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGAGCTTTTCCGGAGTGAGGAGATGACGCTGGCCCAGCTATTTCTACAGTCTGAAGCTGCTTATT
  GCTGTGTCAGTGAATTAGGAGAGCTTGGGAAGGTCCAATTTCGGGATTTAAATCCAGATGTGAATGTTTT
  CCAGAGGAAATTTGTGAATGAAGTTAGAAGATGTGAAGAAATGGATCGAAAACTCCGATTTGTTGAGAAA
  GAGATAAGGAAAGCTAACATCCCAATTATGGACACCGGTGAAAACCCAGAGGTGCCCTTCCCTCGGGACA
  TGATCGACTTAGAGGCCAATTTCGAGAAGATTGAAAATGAACTGAAGGAAATCAACACTAACCAGGAAGC
  TCTGAAGAGAAACTTCCTGGAACTGACTGAATTAAAATTTATCCTGCGAAAAACCCAGCAGTTTTTCGAT
  GAGATGGCGGATCCAGACCTGTTGGAAGAGTCCTCATCACTCTTGGAGCCAAACGAGATGGGAAGAGGCG
  CACCCTTACGACTTGGCTTTGTGGCTGGTGTGATTAACCGGGAGCGGATCCCGACCTTTGAGCGCATGCT
  TTGGCGAGTGTGCCGAGGGAATGTGTTCCTGAGACAAGCTGAAATCGAAAACCCGCTGGAGGATCCTGTG
  ACTGGCGACTACGTGCACAAGTCTGTGTTCATCATTTTTTTCCAAGGCGATCAGCTGAAAAACAGGGTCA
  AGAAAATCTGTGAAGGGTTCCGAGCCTCCCTCTATCCCTGCCCTGAGACTCCACAGGAAAGGAAAGAAAT
  GGCTTCCGGAGTCAATACCAGGATCGACGACCTCCAAATGGTTCTGAATCAGACGGAGGACCACCGCCAG
  AGGGTTCTGCAGGCAGCTGCTAAGAACATCCGCGTCTGGTTCATCAAGGTGCGGAAGATGAAGGCTATCT
  ACCATACCCTGAACCTGTGCAATATAGACGTGACCCAGAAGTGCCTGATCGCAGAAGTCTGGTGCCCTGT
  CACTGACCTGGACTCCATCCAGTTTGCACTACGAAGAGGCACGGAACACAGTGGGTCCACTGTCCCCTCC
  ATTCTGAACAGGATGCAGACAAACCAGACACCGCCGACATATAATAAAACCAACAAGTTCACGCACGGCT
  TCCAGAACATAGTAGATGCTTATGGGATCGGCACTTACCGAGAGATTAACCCAGCTCCGTACACTGTCAT
  CACCTTCCCCTTTCTGTTTGCTGTGATGTTCGGAGATTTTGGGCACGGCATTTTGATGACGCTGTTTGCT
  GTGTGGATGGTGTTGAGGGAGAGCCGGATCCTCTCCCAGAAGCACGAGAATGAGATGTTTAGCATGGTGT
  TCAGCGGCCGGTACATTATTCTTCTGATGGGACTGTTCTCCATCTATACTGGACTCATCTACAATGACTG
  CTTTTCCAAGTCTCTGAATATCTTTGGGTCATCGTGGAGTGTACGGCCAATGTTCACTCAAGGGAACTGG
  ACGGAGGAGACGCTTCTGGGGAGTTCTGTTCTCCAGTTGAACCCCGCTATCCCTGGAGTTTTTGGTGGCC
  CGTACCCGTTTGGCATTGATCCGATTTGGAACATTGCAACCAACAAGCTGACCTTCCTCAACTCCTTCAA
  GATGAAGATGTCTGTTATCCTCGGCATCATCCACATGCTGTTTGGAGTCAGCCTGAGCCTTTTCAACCAT
  ATCTATTTCAAGAAGCCCCTGAACATCTACTTTGGCTTTATTCCTGAGATCATCTTCATGTCCTCGTTGT
  TTGGCTACCTGGTCATCCTTATCTTTTACAAGTGGACAGCCTACGATGCCCACTCGTCTAGGAATGCCCC
  GAGCCTCCTGATCCACTTCATAAACATGTTCCTCTTCTCCTACCCAGAGTCTGGTAATGCAATGCTGTAC
  TCTGGACAGAAAGGAATTCAGTGTTTCCTCATCGTGGTGGCGATGCTCTGCGTCCCTTGGATGCTTCTGT
  TTAAGCCGCTGATCCTTCGCCATCAGTACCTGAGGAAGAAGCATTTGGGAACTCTCAACTTTGGTGGGAT
  CAGGGTGGGCAACGGACCGACAGAGGAGGATGCTGAAATTATTCAGCATGACCAGCTTTCCACCCATTCA
  GAGGACGCAGAAGAGCCTACCGAGGACGAAGTGTTTGACTTTGGCGACACCATGGTCCACCAGGCCATCC
  ACACCATTGAGTACTGCTTGGGTTGTATCTCCAACACCGCGTCGTACCTGCGACTCTGGGCCCTCAGCCT
  GGCCCACGCACAGCTCTCTGAAGTACTTTGGACCATGGTGATCCACATTGGCCTGCACGTCCGGAGTTTG
  GCCGGGGGTCTAGGGCTGTTTTTCATCTTCGCCGCCTTTGCCACGCTGACCGTGGCTATCCTGCTGATCA
  TGGAGGGCCTCTCGGCCTTCCTCCACGCGCTGCGGTTACACTGGGTGGAGTTCCAGAACAAATTCTACAC
  TGGGACTGGCTTCAAGTTCCTCCCCTTCTCCTTTGAGCACATTCGGGAAGGGAAGTTTGATGAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_016920
• Insert Size: 2517 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016920.3, NP_058616.1
• RefSeq Size: 4019 bp
• RefSeq ORF: 2517 bp
• Locus ID: 11975
• UniProt ID: Q9Z1G4
• Cytogenetics: 11 64.04 cM
• Gene Summary: Required for assembly and activity of the vacuolar ATPase. Potential role in differential targeting and regulation of the enzyme for a specific organelle (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longest isoform (1).