Ank3 (NM_170730) Mouse Untagged Clone

CAT#: MC221988

Ank3 (untagged) - Mouse ankyrin 3, epithelial (Ank3), transcript variant 9, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ank3
• Synonyms: 2900054D09Rik; AI314020; An; Ank; Ank-3; AnkG; Anky; Ankyrin-3; Ankyrin-G
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221988 representing NM_170730
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGAAGAGCCAAAGGAGAAGCCCGCCAAGCCTGCTCATAGGAAGAGGAAAGGAAAAAAGCACCTGA
  CGTTCACGAGGGAGTTTGATTCTGACTCCCTCAGACACTACAGTTGGGCAGCGGACACGTTAGATAATGT
  GAACCTGGTCTCAAGCCCGGTGCATTCTGGGTTTCTGGTTAGCTTTATGGTGGACGCGAGAGGGGGCTCC
  ATGCGAGGAAGCCGCCACCACGGGATGCGGATCATCATCCCTCCGCGAAAGTGTACGGCCCCCACCCGCA
  TCACGTGCCGCCTGGTAAAGAGACATAAACTGGCCAACCCACCCCCCATGGTGGAAGGAGAGGGATTAGC
  CAGTAGGCTGGTAGAAATGGGTCCTGCGGGGGCACAATTTTTAGGCCCCGTCATTGTGGAAATCCCTCAT
  TTTGGGTCCATGAGGGGGAAGGAGAGAGAACTTATCGTCCTTCGGAGCGAGAACGGAGAGACCTGGAAGG
  AACATCAGTTTGACAGTAAAAACGAAGACCTCGCGGAGCTTCTCAATGGCATGGATGAAGAACTCGACAG
  CCCGGAAGAGTTGGGTACAAAGCGCATCTGCAGAATTATCACAAAGGATTTCCCCCAGTATTTTGCCGTG
  GTTTCCCGGATTAAGCAGGAAAGCAACCAGATCGGTCCTGAGGGTGGGATTCTGAGCAGCACCACCGTGC
  CCCTCGTCCAGGCCTCCTTCCCAGAGGGCGCCTTAACCAAGAGGATCCGTGTGGGTCTCCAGGCTCAGCC
  CGTGCCAGAGGAAACGGTAAAAAAAATCCTTGGGAACAAAGCAACATTTAGCCCAATTGTCACGGTAGAG
  CCGAGGAGAAGGAAGTTCCATAAGCCGATCACCATGACCATTCCGGTGCCCCCGCCCTCGGGAGAAGGCG
  TGTCCAATGGGTACAAGGGGGATGCCACGCCCAACCTGCGGCTCCTCTGCAGCATCACAGGAGGCACCTC
  ACCAGCTCAATGGGAAGACATCACAGGAACAACCCCTCTGACGTTCATAAAGGATTGTGTGTCTTTCACA
  ACCAACGTTTCAGCCAGATTCTGGCTGGCGGACTGCCATCAGGTGTTAGAGACCGTAGGGCTAGCCTCCC
  AGCTGTACAGAGAGCTGATATGCGTTCCCTACATGGCCAAGTTCGTTGTGTTTGCCAAAACAAACGACCC
  GGTGGAGTCCTCGCTGAGGTGCTTCTGTATGACAGACGACAGGGTGGACAAAACCCTGGAGCAGCAGGAG
  AACTTCGAGGAGGTTGCCAGAAGCAAAGACATTGAGGTTCTGGAAGGAAAGCCCATCTACGTTGATTGCT
  ATGGAAACCTGGCCCCTCTGACCAAAGGAGGACAGCAGCTTGTTTTTAACTTTTATTCTTTCAAAGAAAA
  CAGACTGCCATTTTCCATCAAGATCAGAGACACCAGTCAAGAGCCCTGTGGCCGCCTGTCTTTCCTGAAG
  GAGCCAAAGACAACAAAGGGATTACCCCAAACAGCTGTTTGCAACTTAAATATTACTCTGCCGGCACATA
  AAAAGGCTGAGAAGGCAGACAGACGCCAGAGCTTTGCCTCCCTAGCTTTACGTAAGCGCTACAGCTACTT
  GACTGAACCCAGCATGAGTCCGCAGAGTCCTTGTGAGCGGACGGATATCAGGATGGCGATAGTAGCCGAT
  CACCTGGGACTTAGTTGGACAGAGCTGGCAAGGGAACTGAATTTTTCAGTGGATGAAATCAACCAAATAC
  GTGTGGAAAATCCCAATTCTTTAATTTCTCAGAGCTTCATGTTATTAAAAAAGTGGGTGACCAGAGACGG
  AAAGAATGCCACAACTGATGCCTTAACTTCGGTCTTAACGAAGATTAACCGGATAGACATTGTAACTCTG
  CTGGAAGGACCAATATTTGATTATGGGAATATTTCAGGCACCAGAAGCTTTGCAGATGAAAACAATGTTT
  TCCATGACCCAGTTGATGGTTGGCAGAACGAGACGCCAAGTGGAAGCCTAGAGTCCCCAGCGCAAGCTCG
  AAGACTAACTGGTGGGTTACTGGACCGTCTGGATGACAGCTCTGACCAGGCTCGGGATTCTATTACCTCA
  TACCTCACGGGAGAACCTGGGAAGATCGAAGCAAATGGAAACCACACAGCGGAAGTCATTCCAGAAGCAA
  AGGCAAAACCCTACTTCCCGGAATCCCAAAACGATATAGGGAAACAGAGCATCAAGGAGAACCTGAAACC
  AAAAACACACGGATGTGGTCGCACTGAGGAACCAGTGTCGCCCCTCACAGCCTACCAGAAATCTCTGGAA
  GAAACCAGCAAGCTTGTCATAGAAGACGCACCTAAACCCTGTGTGCCTGTCGGCATGAAAAAGATGACCA
  GGACTACGGCTGACGGCAAAGCCAGGCTCAACCTCCAGGAAGAAGAGGGGTCCACCAGGTCAGAGCCTAA
  GCAGGGAGAAGGCTATAAGGTGAAGACGAAGAAGGAAATCCGGAACGTGGAGAAGAAAACCCACTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_170730
• Insert Size: 2517 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_170730.2, NP_733926.2
• RefSeq Size: 6656 bp
• RefSeq ORF: 2517 bp
• Locus ID: 11735
• UniProt ID: G5E8K5
• Cytogenetics: 10 36.1 cM
• Gene Summary: This gene encodes a member of the ankyrin protein family. Ankyrins link integral membrane proteins to the spectrin-based cytoskeleton. Ankyrin family members share a protein structure which includes three independently folded domains: the N-terminal ankyrin repeat domain, the central spectrin-binding domain, and the C-terminal rod domain. This ankyrin functions as the major ankyrin in the kidney and may play a role in the polarized distribution of many integral membrane proteins to specific subcellular sites. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (9) lacks an in-frame segment in the 5' coding region and an in-frame segment in the 3' coding region, compared to variant 2. This results in a shorter isoform (i), compared to isoform b.