Plb1 (NM_030072) Mouse Untagged Clone

CAT#: MC221979

Plb1 (untagged) - Mouse phospholipase B1 (Plb1), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Plb1
• Synonyms: 4632413E21Rik; 4930433E17Rik; 4930539A06Rik; BC033606
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221979 representing NM_030072
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAAACCTCAAAGCATCAAGAACCAACCACAGGATGTATGCATGGGAATCATGACAGTTCTTTCAG
  ATATCATCAGACATTTCAGTCCTTCTGTCCTGATGCCCACGTGTTCTCCTGGGAAGGGCACTGCAGTCCA
  CACTACTGCTGAAGATTTGTGGATTCAGGCTAAAGAGCTGGTGAGGCGCCTGAAGGACAACCCGCAACTT
  GACTTTGAGAAGGACTGGAAACTCATCACTGTGTTCTTCAGTAACACAAGCCAGTGTCACCTGTGTCCCT
  CTGCTCAGCAGAAAAGTCACTTGATGAGGCACATGGAGATGCTGTGGGGGGTGCTGGATTACCTGCATCA
  TGAGGTCCCCAGAGCATTTGTGAATTTGGTGGATCTCTCTGAGGTTTTGGCCATGGATCTTCAGCATCAA
  GAGACTGGCTTCAGCCCTGCACCAGAGGTTTGCAAATGCACAGAGACAACGACGCTATCCAAAGCTGTCA
  TGCAGTGGTCCTATCAGGAAGCCTGGGAAGATCTTCTGGCCTCTAGCAAGTTCAACAAGCATGAGACCTT
  CGCGGTGGTTTTCCAGCCTTTCTTCGATGAAATTGAACCACCCTTGAAAAGGTCCTCGCCCCAGGATCCC
  ACCACACTCGCCCTCAGGATCTGGAATAGCATGATGGAACCAGTGGGTCAAAAGGATGGGCTACTCAATA
  CTGCAGAGAGAAAGACAATGAAATGTCCCTCTGAGGAGAGTCCCTATCTGTTCACCTACAAAAATAGCAA
  CTACCAGGCCAGACGACTGAAACCTATAACAAAGCTTCAGATGAAAGAAGGATCAGAATTCACCTGTCCT
  GACAAGAACCCCTCGAACTCAATCCCCACAACAGTTCACAGCTTGCGGCCAGCTGATATCAAGATCATAG
  GAGCATTGGGTGACTCGCTCACGGCAGGCAATGGAGCTGGGGCCAGCCCTTGGAATATCTTGGATGTCTT
  AACTGAATACCGAGGCCTGTCCTGGAGTGTGGGCGGGGATGAGACCATCAAAACCGTGACCACCCTACCC
  AACATTCTCCGGGAATTCAACCCCTCCTTGAAGGGCTTTTCTGTTGGCACTGGGAAAGAAAGCACTTCCC
  GAGCATCCTTCAACCAGGCCGTAGCAGGAGCCAAATCTGATGGCTTAGCTGGCCAGGCCAGAAAGCTGGT
  GGACCTGATGAAGGCAGACAAGACAATAAACTTTCAGGAAGACTGGAAGATAATCACTGTGTTTATAGGA
  GGCAATGACCTCTGTGCTTCCTGCAGTAACTCGACTCGCTTTTCTCCCCAAAACTTCATAGACAACATCA
  AGAACGCCCTGGACATCCTCCATGCAGAGGTTCCACGGGCCTTTGTGAACATGGCAATGGTGATGGAGAT
  CACCCCCTTGAGAGAACTATTCAATGAACCTACAGTCTCATGCCCACGGAACATCCTCAGCCGACTGTGT
  CCTTGTGTCCTGGGCCTTGGTGATAACTCAGAAGAACTTTCCAGCCTCGTTCAAAGGAACAGGGATTATC
  AGAAGAAAACTGAAGAGCTCATTAATAGCGGACGATATGACACGAGGGATAATTTCACTGTGGTTGTCCA
  GCCCTTATTTGAAAATGTGTCCATGCCACGGACCCCGGAGGGCGTGCCCGACAAGTCTTTCTTTGCCCCT
  GACTGTTTCCACTTTAATGCCAAGACTCATGCCCGCTCAGCCATTGCCCTCTGGAAGAACATGCTGGAAC
  CTGTGGGCCACAAGACAAGACATAATAATTTTGAAATCAAGGCCCCTATCGTGTGTCCGAACCAGGCCTC
  GCCATTTCTGAGCACTACCAAGAACAGCAACCTGGGTAATGGAACCTGGATGGTGTGCGAGGAGAGAGCC
  CCTTCTGCCTCACCACCAACCTCAGTGCATACCCTGAGACCTGCAGACATTCAAGTTGTGGCAGCTCTGG
  GAGACTCTCTGACTGCTGGCAATGGAATCAGCTCCCAAGAAGGTAACCTCACCGATGTCTCCACACAGTA
  TCGAGGACTGTCCTATAGTGCTGGTGGGGACAAGACCCTGGAGAATGTGACCACTTTGCCCAATATCCTA
  CGGAAATTTAATGGAAATCTCACAGGCTACTCAGTAGGAACCGGTGACTCCAGTTCCGCAAACGCTTTCC
  TTAACCAGGCTGTCCCTGGGGCGAAGGCTGAGAACCTTACAAGCCAAGTCCGGACTCTGGTTCAGAAGAT
  GAAGAGTGACAACAGAGTGAACTTCAACCGAGACTGGAAGGTCATCACGGTGATGATCGGCGCCAGTGAC
  TTGTGTGACTTCTGCACGGATTCGAATCATTACTCTGCAGCCAATTTTTTTGACCATCTCCAAAATGCCT
  TGGACATTCTACATAAGGAGGTACCCAGAGCCCTGGTCAACCTTGTGGACTTCATTAACCCCAGTATCAT
  TCGGGAAGTATTCCTGAAGAACCCAGACAAGTGCCCAGTGAATCAGTCCAGGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_030072
• Insert Size: 2505 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030072.1, NP_084348.1
• RefSeq Size: 3147 bp
• RefSeq ORF: 2505 bp
• Locus ID: 665270
• UniProt ID: Q3TTY0
• Cytogenetics: 5 B1
• Gene Summary: Membrane-associated phospholipase. Exhibits a calcium-independent broad substrate specificity including phospholipase A2/lysophospholipase activity. Preferential hydrolysis at the sn-2 position of diacylphospholipids and diacyglycerol, whereas it shows no positional specificity toward triacylglycerol. Exhibits also esterase activity toward p-nitrophenyl. May act on the brush border membrane to facilitate the absorption of digested lipids (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) has multiple differences and uses a downstream start codon, compared to variant 1. The resulting protein (isoform 2) has shorter N- and C-termini when compared to isoform 1.