Jade1 (NM_001130184) Mouse Untagged Clone

CAT#: MC221970

Phf17 (untagged) - Mouse PHD finger protein 17 (Phf17), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Jade1
• Synonyms: AU041499; D530048A03Rik; mKIAA1807; Phf17
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221970 representing NM_001130184
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAACGAGGTCGCCTTCCCAGCAGCAGTGAGGATTCTGACGACAATGGCAGCTTGTCAACGACATGGT
  CCCAGCATTCTCGATCCCAGCATGGGAGGAGTAGTACCTGCTCTAGACCTGAAGATCGAAAGCCTTCTGA
  GGTGTTTAGGACCGACCTGATCACTGCCATGAAGTTGCATGACTCCTACCAGCTGAACCCGGATGACTAC
  TACGTGCTGGCTGATCCCTGGAGACAGGAGTGGGAGAAAGGGGTCCAGGTGCCTGTGAGCCCAGGGACCA
  TCCCACAGCCTGTGGCCAGGGTTGTGTCTGAAGAGAAGTCTCTCATGTTCATCAGGCCCAAGAAGTACAT
  CGCGTCGTCCGGCTCTGAGCCCCCAGCGTTAGGCTACGTCGATATCCGGACTCTGGCAGACAGTGTGTGT
  CGGTATGACCTCAATGATATGGATGCTGCGTGGCTGGAAGTAACCAATGAAGAATTTAAGGAGATGGGGA
  TGCCGGAGCTGGACGAGTACACCATGGAAAGGGTCTTGGAGGAATTCGAACAGCGATGCTATGACAATAT
  GAATCATGCCATAGAGACAGAAGAGGGCTTGGGGATTGAATACGACGAAGACGTTGTCTGCGATGTCTGC
  CAGTCACCTGATGGCGAGGACGGCAACGAGATGGTGTTCTGTGACAAGTGTAACATCTGTGTGCACCAGG
  CTTGCTATGGGATCCTCAAGGTGCCAGAGGGCAGTTGGCTATGTCGTACATGTGCCCTGGGAGTTCAGCC
  AAAATGTTTGCTGTGTCCCAAGAAAGGCGGAGCTATGAAGCCGACCCGCAGCGGAACCAAGTGGGTCCAC
  GTCAGCTGTGCCCTGTGGATCCCTGAGGTAAGCATTGGCAGCCCTGAGAAGATGGAGCCCATCACAAAAG
  TGTCTCACATTCCCAGCAGCCGGTGGGCGCTTGTGTGCAGCCTCTGCAATGAGAAATTCGGGGCCTCCAT
  ACAGTGCTCTGTGAAGAACTGCCGGACAGCCTTCCACGTGACTTGTGCTTTTGACCGTGGCCTGGAGATG
  AAGACCATATTGGCAGAGAATGATGAAGTCAAATTCAAGTCCTACTGCCCAAAGCACAGCTCACACAGGA
  AACCCGAGGAGGGCCTGGGTGAAGGAGCAGCCCAGGAGAATGGGGCCCCTGAGAGTTCTCCCCAGAGCCC
  TCTGGAGCCCTATGGTAGTCTGGAGCCGAATCGAGAGGAGGCCCACCGGGTGAGTGTTCGCAAGCAGAAG
  CTACAGCAGCTGGAGGATGAGTTCTACACCTTTGTCAACCTGCTGGATGTGGCCAGGGCGCTGCGGCTGC
  CTGAGGAAGTGGTGGATTTCCTGTACCAGTACTGGAAGTTGAAGAGGAAGATCAACTTCAACAAGCCCCT
  CATCACCCCAAAGAAAGACGAAGAGGACAATCTAGCCAAGCGGGAGCAGGATGTCTTGTTTAGGAGGCTG
  CAGCTGTTCACGCACCTGCGGCAGGACCTGGAGAGGGTTCGGAACCTCACTTACATGGTGACCCGCAGGG
  AAAAGATTAAACGGTCTGTGTGCAAAGTCCAGGAACAAATTTTCACTCAGTACACTAAGCTCTTGGAGCA
  AGAAAAAGTTTCAGGTGTGCCTTCTTCCTGCTCCTCCGCACTGGAGAACATGCTTTTTTTCAACAGTCCT
  TCTGTGGGCCCCAATGCTCCCAAGATAGAGGACTTGAAATGGCATTCTGCATTCTTCAGGAAACAAATGG
  GCACTTCCTTGGTTCATCCACTGAAAAAGTCCCATAAACGAGATGCAGTGCAGAATAGTTCTGGGACCGA
  GGGCAAGACCTCGCATAAGCAGCCAGGTCTTTGTGGTAGAAGGGAGGGGCTGGAGGTCTCAGAAAGCTTG
  CTGAGCTTAGAAAAGACTTTTGCAGAAGCACGTCTCCTATCATCAGCACAACAGAAAAATGGTGTGGTGA
  CCCCAGACCATGGGAAAAGAAGAGACAATCGTTTTCATTGTGATCTCGTTAAAGGAGACTTAAAGGACAA
  ATCTTTTAAGCAGAGTCACAAGCCTCTCAGGTCCACAGACACATCCCAGAGGCATCTGGACAACACAAGA
  GCTGCCACCTCCCCTGGAGTAGGGCAGTCAGCACCTGGCACCAGGAAGGAGATTGTGCCCAAGTGCAATG
  GCTCCCTAGTCAAAGTGCCTATAACACCTGCCAGCCCAGTGAAAAGCTGGGGAGGATTCCGGATTCCAAA
  GAAGGGGGAGCGGCAGCAGCAAGGAGAAGCCCATGATGGGGCCTGCCACCAGCACTCAGACTGCTCCCAT
  CTGGGTGTAAGCCGAGCTCCAGCCAAGGAGAGAGCGAAGAGCAGGTTAAGAGCTGACAGTGAGAATGATG
  GGTACGCCCCTGATGGGGAAATGAGTGACTCAGAGAGCGAGGCATCAGAGAAGAAATGTATCCATGCCAG
  CAGCACCATCAGCAGGAGGACAGATATCATCAGGCGAAGCATCTTGGCCTCTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001130184
• Insert Size: 2505 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001130184.1, NP_001123656.1
• RefSeq Size: 5486 bp
• RefSeq ORF: 2505 bp
• Locus ID: 269424
• UniProt ID: Q6ZPI0
• Cytogenetics: 3 B
• Gene Summary: Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. Transcriptional coactivator, it may also promote acetylation of nucleosomal histone H4 by KAT5. Promotes apoptosis. May act as a renal tumor suppressor. Negatively regulates canonical Wnt signaling; at least in part, cooperates with NPHP4 in this function (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. All four variants encode the same protein.