Nlgn2 (NM_198862) Mouse Untagged Clone

CAT#: MC221962

Nlgn2 (untagged) - Mouse neuroligin 2 (Nlgn2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nlgn2
• Synonyms: NL2; NLG2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221962 representing NM_198862
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGCTCCTGGCGTTGTGTCTGGTGGGGCTGGCTGGGGCTCAACGGGGAGGAGGGGGTCCCGGCGGCG
  GCGCCCCGGGCGGCCCAGGCCTGGGCCTCGGCAGCCTCGGGGAGGAGCGCTTCCCCGTGGTGAACACAGC
  CTACGGGCGAGTGCGCGGTGTGCGGCGCGAGCTCAACAACGAGATCCTGGGCCCGGTCGTGCAGTTCTTG
  GGCGTGCCCTACGCCACGCCGCCCTTGGGCGCCCGCCGCTTCCAGCCGCCTGAGGCACCTGCCTCGTGGC
  CCGGCGTGCGCAACGCCACCACCCTGCCGCCCGCCTGCCCGCAGAACCTGCACGGGGCCCTGCCGGCCAT
  CATGCTGCCTGTGTGGTTCACCGACAACTTGGAGGCGGCCGCCACCTACGTGCAGAACCAGAGCGAGGAC
  TGCCTGTACCTCAACCTCTACGTGCCCACTGAGGACGGTCCGCTCACAAAAAAACGTGACGAGGCGACGC
  TCAATCCGCCAGACACAGATATCCGGGACTCTGGGAAGAAACCGGTCATGCTGTTTCTACACGGCGGCTC
  CTACATGGAAGGCACCGGGAACATGTTTGACGGCTCAGTCCTGGCTGCCTATGGCAATGTCATCGTAGTC
  ACACTCAACTACCGTCTTGGGGTGCTCGGTTTTCTCAGCACTGGTGACCAGGCTGCAAAAGGCAACTATG
  GGCTCCTGGACCAGATCCAGGCCCTGCGCTGGCTCAGTGAAAACATTGCCCACTTTGGAGGTGACCCTGA
  ACGCATCACTATCTTTGGGTCTGGTGCAGGGGCCTCCTGTGTCAACTTGCTGATCCTTTCCCACCACTCA
  GAAGGACTGTTCCAGAAGGCCATTGCTCAAAGTGGTACTGCCATTTCCAGCTGGTCTGTCAACTACCAGC
  CGCTCAAGTACACGCGGCTGCTGGCGGCCAAAGTGGGCTGTGACCGAGAAGACAGCACTGAAGCTGTGGA
  GTGTCTGCGCCGGAAGTCTTCCCGGGAGCTAGTGGACCAGGATGTACAGCCTGCCCGCTACCACATTGCC
  TTTGGGCCTGTGGTGGACGGCGACGTAGTCCCTGATGACCCCGAGATCCTCATGCAACAGGGGGAATTCC
  TCAACTACGACATGCTCATTGGTGTCAACCAGGGAGAGGGTCTCAAGTTCGTGGAGGACTCTGCAGAGAG
  TGAGGACGGTGTGTCTGCCAGCGCCTTTGACTTCACCGTCTCCAACTTTGTGGACAACTTGTACGGGTAC
  CCAGAAGGCAAGGACGTGCTTCGAGAGACCATCAAGTTCATGTACACGGACTGGGCTGACAGGGACAATG
  GCGAGATGCGGCGGAAGACCCTGCTGGCGCTCTTTACCGACCACCAGTGGGTCGCCCCGGCTGTGGCCAC
  CGCCAAGCTGCATGCCGACTACCAGTCCCCCGTCTACTTTTACACTTTCTACCACCACTGCCAGGCAGAG
  GGCCGGCCAGAGTGGGCAGACGCAGCGCACGGGGACGAGCTGCCCTACGTCTTTGGTGTCCCCATGGTGG
  GCGCCACTGACCTCTTCCCCTGCAACTTCTCCAAGAACGATGTCATGCTCAGCGCAGTAGTCATGACCTA
  TTGGACCAACTTCGCCAAGACTGGTGACCCCAACCAGCCTGTGCCACAGGACACCAAGTTCATCCACACC
  AAGCCCAACCGCTTTGAAGAGGTAGTGTGGAGCAAGTTCAACAGCAAGGAAAAGCAGTATCTGCACATAG
  GCTTGAAACCACGCGTGCGCGACAACTACCGTGCCAACAAGGTGGCCTTCTGGCTGGAGCTCGTGCCCCA
  CCTGCACAACCTGCACACAGAGCTCTTCACCACCACCACTCGCCTGCCTCCCTATGCCACACGCTGGCCA
  CCTCGCACACCTGGTCCTGGCACTTCCGGCACACGCCGTCCTCCCCCACCTGCCACTCTGCCACCTGAGT
  CTGATATTGACCTAGGCCCAAGGGCCTATGACCGCTTCCCCGGTGACTCGAGGGACTACTCCACGGAGCT
  AAGCGTGACTGTGGCAGTGGGTGCCTCCCTCCTCTTCCTCAACATCCTTGCCTTTGCCGCCCTCTATTAC
  AAGCGGGACCGGCGCCAGGAGCTGCGGTGCCGGAGGCTTAGCCCACCAGGAGGCTCAGGCTCAGGTGTGC
  CTGGTGGGGGCCCCCTGCTTCCCACTGCTGGCCGTGAGCTACCCCCGGAGGAGGAGCTAGTATCGCTGCA
  GCTGAAGCGGGGTGGTGGTGTTGGGGCGGACCCTGCTGAGGCCCTGCGCCCTGCCTGTCCACCCGACTAT
  ACCCTGGCCTTGCGCCGGGCACCGGACGATGTGCCTCTCTTGGCCCCCGGGGCCCTAACCCTGCTGCCTA
  GTGGCCTGGGGCCCCCGCCCCCGCCCCCACCCCCTTCTCTCCATCCCTTTGGGCCCTTCCCACCCCCACC
  CCCTACTGCTACCAGCCACAACAACACGCTACCCCATCCCCACTCCACCACTCGGGTATAG
  
  CAAGCTTAACTAGCTAGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATC
  TGGCAGCAAATGATATCCTGGATTACAAGGATGACGACGATAAGGTTTAAAC
• Restriction Sites: SgfI-HindIII
• ACCN: NM_198862
• Insert Size: 2511 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198862.2, NP_942562.2
• RefSeq Size: 5034 bp
• RefSeq ORF: 2511 bp
• Locus ID: 216856
• UniProt ID: Q69ZK9
• Cytogenetics: 11 B3
• Gene Summary: Transmembrane scaffolding protein involved in cell-cell interactions via its interactions with neurexin family members. Mediates cell-cell interactions both in neurons and in other types of cells, such as Langerhans beta cells. Mediates cell-cell interactions between Langerhans beta cells and modulates insulin secretion (By similarity). Plays a role in synapse function and synaptic signal transmission, especially via gamma-aminobutyric acid receptors (GABA(A) receptors). Functions by recruiting and clustering synaptic proteins. Promotes clustering of postsynaptic GABRG2 and GPHN. Promotes clustering of postsynaptic LHFPL4 (PubMed:29742426). Modulates signaling by inhibitory synapses, and thereby plays a role in controlling the ratio of signaling by excitatory and inhibitory synapses and information processing. Required for normal signal amplitude from inhibitory synapses, but is not essential for normal signal frequency. May promote the initial formation of synapses, but is not essential for this. In vitro, triggers the de novo formation of presynaptic structures.[UniProtKB/Swiss-Prot Function]