Jade2 (NM_199299) Mouse Untagged Clone

CAT#: MC221953

Phf15 (untagged) - Mouse PHD finger protein 15 (Phf15), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Jade2
• Synonyms: 1200017K05Rik; AI480685; mKIAA0239; Phf15
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221953 representing NM_199299
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGAGAAGAGGCGAAAATATTCCATCAGCAGCGACAACTCTGATACCACTGACGGTCACGTGACAT
  CCACATCAGCATCAAGATGTTCCAAACTGCCCAGCAGCACCAAGTCGGGCTGGCCCCGGCAGAACGAGAA
  GAAGCCCTCAGAGGTTTTCCGGACAGACTTGATCACAGCCATGAAGATCCCAGATTCATACCAGCTCAGC
  CCGGATGACTACTACATCCTGGCGGACCCGTGGCGACAAGAATGGGAGAAAGGGGTGCAGGTACCTGCTG
  GAGCGGAGGCCATTCCAGAGCCTGTGGTGAGGCTCCTCCCACCACTGAAAGGCCCCCCCACGCAGATGTC
  CCCAGATAGCCCCACACTTGGTGAGGGTGCCCATCCTGACTGGCCAGGAGGCAGCCGCTACGACCTGGAT
  GAGATCGATGCGTACTGGTTGGAACTTCTCAACTCGGAGCTCAAGGAGATGGAGAAGCCCGAGCTGGATG
  AGCTAACGTTAGAGCGTGTTCTAGAGGAGCTAGAGACATTGTGCCACCAGAATATGGCACAGGCCATTGA
  GACACAGGAGGGGCTGGGCATCGAGTACGACGAGGACGTTGTCTGCGACGTGTGCCGTTCCCCTGAAGGC
  GAGGATGGCAACGAGATGGTCTTCTGTGACAAATGCAATGTCTGTGTGCACCAGGCATGCTACGGGATCC
  TCAAGGTGCCTACGGGCAGCTGGCTGTGCCGGACCTGTGCCCTGGGAGTCCAGCCTAAGTGCCTGCTCTG
  CCCCAAGCGAGGAGGAGCCCTGAAGCCCACTAGAAGTGGGACCAAGTGGGTACACGTCAGCTGTGCCCTG
  TGGATTCCTGAGGTCAGCATTGGCTGTCCAGAGAAGATGGAGCCCATTACCAAGATCTCGCATATTCCGG
  CCAGCCGCTGGGCCCTGTCCTGCAGCCTCTGCAAGGAGTGCACAGGTACCTGCATCCAGTGTTCCATGCC
  TTCCTGCATCACAGCATTCCACGTTACGTGCGCCTTTGACCGAGGCCTGGAAATGCGGACTATATTAGCT
  GACAATGACGAGGTCAAGTTCAAGTCACTTTGCCAGGAGCACAGTGACGGGGGCCCTCGGAGTGAGCCTA
  CTTCTGAGCCTGTGGAGCCCAGCCAGGCCGTTGAGGATCTGGAAAAGGTGACCTTACGCAAGCAGCGGCT
  GCAGCAGCTGGAAGAAAACTTCTATGAGCTAGTGGAGCCAGCTGAGGTGGCTGAACGGCTAGACCTGGCT
  GAGGCACTGGTGGACTTCATCTACCAGTACTGGAAGTTGAAGCGGAGAGCTAATGCCAACCAGCCGCTGT
  TGACGCCCAAGACTGACGAGGTGGACAACCTGGCCCAACAGGAACAGGATGTCCTCTATCGACGCCTGAA
  GCTTTTCACCCACCTGCGGCAGGACCTGGAGAGGGTAAGGAACCTGTGCTACATGGTGACAAGACGGGAG
  AGAACGAAACACACCATCTGTAAACTTCAGGAGCAGATATTCCATCTACAGATGAAACTTATTGAGCAAG
  ACCTTTGCAGAGAGCCTTCTGGGAGGAGGTCAAAGGGCAAGAAGAATGATTCAAAAAGGAAAGGCCGAGA
  GGGTCCCAAGGGCAGCAGCCCTGAGAAGAAAGAGAAAGTGAAGGCTGGGCCCGAGTCTGTGCTGGGGCAG
  CTGGGTCTATCCACCTCGTTCCCCATCGACGGCACTTTCTTCAACAGCTGGTTGGCACAGTCGGTTCAGA
  TCACAGCAGAGGACATGGCCATGAGCGAGTGGTCTTTGAACAGTGGGCACCGGGAGGATCCTGCTCCAGG
  TCTGCTGTCAGAGGAATTGCTACAAGATGAGGAGACGCTGCTCAGCTTCATGAGGGACCCCTCGCTACGA
  CCTGGTGACCCTGCCAGAAAGGCCCGAGGCCGCACTCGCCTGCCTGCCAAGAAGAAACCATCCCCGCTGC
  AGGATGGGCCCAGTGCACGGACCACTCCAGACAAGCAACCCAAGAAGGCCTGGGCCCAGGATGGCAAGGG
  GACGCAAGGACCACCCATGAGGAAGCCACCACGGAGGACGTCTTCTCATTTGCCGTCCAGCCCTGCAGCT
  GGGGACTGTCCAGTCCCAGCAACACTGGAAAGCCCTCCACCACTGGCCTCCGAGATACTAGACAAGACAG
  CCCCCATGGCTTCCGACTTAAATGTCCAAGTGCCTGGCCCTACAGTGAGCCCCAAACCCTTGGGCAGGCT
  CCGGCCACCCCGAGAGATGAAGGTCAGTCGGAAATCTCCGGGTGCTAGATCCGATGCTGGGACAGGACTA
  CCGTCTGCTGTGGCCGAGAGGCCAAAGGTCAGCCTGCATTTTGACACCGAGGCTGACGGCTACTTCTCTG
  ATGAGGAGATGAGCGATTCTGAGGTAGAGGCAGAGGACAGTGGGGTACAACGAGCTTCCAGGGAGGCAGG
  GGCAGAGGAGGTGGTTCGCATGGGGGTGCTGGCCTCCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_199299
• Insert Size: 2490 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_199299.3, NP_955003.2
• RefSeq Size: 6100 bp
• RefSeq ORF: 2490 bp
• Locus ID: 76901
• UniProt ID: Q6ZQF7
• Cytogenetics: 11 B1.3
• Gene Summary: Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo (By similarity). Acts as a E3 ubiquitin-protein ligase mediating the ubiquitination and subsequent proteasomal degradation of target protein histone demethylase KDM1A. Also acts as a ubiquitin ligase E3 toward itself. Positive regulator of neurogenesis (PubMed:25018020).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR and 5' coding region and initiates translation at a downstream start codon, compared to variant 1. It encodes isoform 2, which has a shorter N-terminus, compared to isoform 1.