Sidt2 (NM_172257) Mouse Untagged Clone

CAT#: MC221923

Sidt2 (untagged) - Mouse SID1 transmembrane family, member 2 (Sidt2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sidt2
• Synonyms: B930096O19; BC023957; CGI-40
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221923 representing NM_172257
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCGCCTGGCGTCTGCCCTTGTGCGTGCTCTTGGTGGCCTCCGTCGAGAGCCACCTGGGGGCCCTGG
  GGCCCAAGAACGTCTCGCAGAAAGACGCGGAGTTTGAGCGCACCTACGCGGACGACGTCAACAGCGAGCT
  GGTCAACATCTACACCTTCAACCACACCGTGACCCGCAACCGGACCGAGGGTGTGCGAGTGTCTGTGAAT
  GTCCTGAACAAGCAGAAAGGGGCGCCTTTGCTGTTTGTGGTCCGCCAGAAGGAGGCTGTTGTGTCCTTCC
  AGGTGCCCCTAATCCTTCGAGGACTATATCAGCGGAAGTACCTCTACCAAAAAGTGGAACGAACTCTGTG
  TCAGCCCCCCACCAAGAATGAGTCTGAGATCCAGTTTTTCTATGTGGACGTGTCTACCCTGTCACCCGTC
  AATACCACTTACCAGCTCCGAGTCAACCGTGTGGACAATTTTGTGCTCAGGACTGGAGAGCTGTTTACTT
  TTAATACCACTGCAGCCCAGCCCCAGTACTTCAAATACGAGTTTCCTGATGGTGTGGACTCGGTAATTGT
  CAAGGTGACCTCCAAGAAGGCCTTCCCCTGCTCAGTCATCTCCATCCAAGATGTCCTGTGCCCTGTCTAT
  GATCTGGACAACAATGTAGCCTTCATTGGCATGTACCAGACGATGACTAAGAAGGCAGCCATCACTGTGC
  AGCGGAAAGACTTCCCCAGCAACAGCTTCTATGTGGTGGTGGTAGTGAAGACTGAGGACCAGGCCTGCGG
  AGGGTCCTTGCCCTTCTACCCTTTTGTGGAAGATGAGCCAGTGGATCAAGGGCACCGTCAGAAAACACTG
  TCAGTGCTGGTCTCTCAGGCTGTCACATCTGAGGCCTATGTTGGTGGGATGCTCTTTTGCCTGGGCATAT
  TCTTGTCCTTCTACCTGCTGACTGTGCTGCTGGCCTGTTGGGAGAACTGGAGGCAAAGGAAGAAGACCTT
  GCTGTTGGCCATAGACCGAGCCTGCCCAGAAAGTGGTCACGCCCGGGTCTTGGCTGATTCATTTCCTGGC
  AGTGCCCCTTACGAGGGTTACAACTATGGCTCCTTTGAAAATGGTTCCGGATCCACTGACGGGTTGGTTG
  AAAGCGCAGGTTCAGGGGACCTCTCCTACAGTTACCAGGACCGCTCCTTTGACGCAGTGGGTCCTCGGCC
  TCGACTGGACTCCATGAGCTCCGTGGAAGAGGATGACTACGACACACTGACTGACATCGACTCAGACAAA
  AACGTCATTCGAACCAAGCAATACCTCTGTGTGGCTGATCTGGCACGAAAGGACAAACGTGTTTTGCGGA
  AAAAGTACCAGATTTACTTCTGGAACATAGCCACCATTGCGGTCTTCTACGCACTTCCTGTGGTGCAGCT
  GGTGATCACCTACCAGACGGTGGTGAATGTCACAGGGAACCAGGACATCTGCTACTACAACTTCCTCTGT
  GCCCACCCGCTGGGCAACCTCAGCGCCTTCAACAACATCCTCAGCAACTTGGGGTACATCCTGCTGGGGC
  TGCTCTTCCTGCTCATCATCCTGCAGCGAGAGATCAATCATAACCGGGCCCTGCTGCGGAATGACCTCTA
  TGCTCTGGAGTGTGGGATCCCCAAACACTTTGGTCTGTTTTACGCCATGGGCACAGCACTGATGATGGAG
  GGGCTACTTAGTGCCTGTTACCACGTCTGCCCCAACTACACCAACTTCCAGTTTGATACCTCCTTCATGT
  ACATGATTGCTGGCCTCTGCATGCTGAAGCTCTACCAGAAGCGGCACCCAGATATCAACGCCAGTGCCTA
  CAGTGCATATGCCTGCTTGGCCATCGTCATCTTCTTCTCCGTTCTGGGCGTGGTGTTTGGCAAAGGGAAC
  ACGGCCTTCTGGATTGTCTTCTCCGTCATTCACATCATCTCCACCCTGCTCCTCAGCACTCAGCTCTATT
  ACATGGGCCGCTGGAAGCTGGACTCCGGGATCTTCCGCCGCATCCTCCATGTGCTCTACACAGACTGCAT
  CCGGCAGTGCAGCGGGCCCCTTTACACGGACCGCATGGTGCTTCTGGTCATGGGCAACATTATCAACTGG
  TCGCTGGCTGCATACGGACTCATCATGCGCCCCAATGACTTTGCTTCCTACTTGCTGGCAATTGGCATCT
  GCAACCTGCTGCTTTATTTCGCCTTCTACATCATCATGAAGCTCCGGAGCGGCGAGAGGATCAAGCTCAT
  CCCTCTGCTCTGCATCGTCTGCACCTCCGTGGTCTGGGGCTTCGCGCTCTTCTTCTTCTTCCAGGGACTG
  AGCACGTGGCAGAAAACCCCCGCAGAGTCCAGGGAGCACAACCGCGACTGCATCCTCCTCGACTTCTTTG
  ATGACCACGATATCTGGCACTTCCTGTCCTCCATTGCCATGTTTGGGTCCTTCCTGGTTTTGCTGACGTT
  GGATGACGACTTGGACACAGTACAGCGGGACAAGATCTATGTCTTCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_172257
• Insert Size: 2499 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172257.4, NP_758461.1
• RefSeq Size: 4239 bp
• RefSeq ORF: 2499 bp
• Locus ID: 214597
• UniProt ID: Q8CIF6
• Cytogenetics: 9 A5.2
• Gene Summary: Mediates the translocation of RNA and DNA across the lysosomal membrane during RNA and DNA autophagy (RDA), a process in which RNA and DNA is directly imported into lysosomes in an ATP-dependent manner, and degraded (PubMed:27046251, PubMed:27846365, PubMed:28724756). Involved in the uptake of single-stranded oligonucleotides by living cells, a process called gymnosis (PubMed:28277980). In vitro, mediates the uptake of linear DNA more efficiently than that of circular DNA, but exhibits similar uptake efficacy toward RNA and DNA (PubMed:27846365). Binds long double-stranded RNA (dsRNA) (500 - 700 base pairs), but not dsRNA shorter than 100 bp (PubMed:26067272).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an exon in the coding region, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.