L3mbtl1 (NM_001081338) Mouse Untagged Clone

CAT#: MC221900

L3mbtl1 (untagged) - Mouse l(3)mbt-like (Drosophila) (L3mbtl1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: L3mbtl1
• Synonyms: C630004G01; L3mbtl; mKIAA0681
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221900 representing NM_001081338
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGGGCACACTGACATGGAGATACTAAGGACAGTGAAAGGGTCTTCCACAGGGGAGGTCAACGTGC
  ACCTGGTGGCCAGAGACAGCGCAGGTCCCCACCCTCAGCTGCCTACAACCGCCTTCATTATTCCTACCAA
  TGCAGCTACGCTTGGTCTGCCTAGCACTGCCCTGGATGTGCCCTATCCCCGGGAGCCAGTCCACGTGGGT
  GCCCTGGAGCGGGTGGCTGGCAGCGAACCCGTAACAGCTACTATTCTGCCACAGCTAAGCACTGGAACGG
  GGACCAATAGCACAGTCAGGCTTCTGGATTGGACTGGCGTTTCTGCACCTTTACCTGGTAGCGGCATGCG
  GTTCCGGATAAATGAGTACGCGCCACTGAACATGATAGGAGTGGAGCGACCACGAAGCCCGGAGCAGCGA
  CATGAGGGTGGCATGGCCAGGAGAGATGCGGGCATCCAGCACCCTGATGTTCACCAGGACCGTCAAGATA
  TAACTTCTCTGGAACCCCCGGTAGATGCGAGTTCCTGCAAGTGCCAGGCTTGTGGGCCTCAACAAAGTAG
  TGGCCTAGACGTTGGTTCATCGGGAGACCGATGCTCTCAGCCTTTCCAGAAGCGGTCAGTCATAGTAGAG
  AACTCAGGCTGTACCATCGCTTCTGAGCTCCTGAAACCCATGAAAAAGAGGAAACATAAGGAGTATCAGA
  GCCCATCAGAGGAGTCAGAGCCGGAGGCTGTGAAGCAAGGAGAAGGAAAAGACGCAGAGCGAGAGCCCAC
  CCCTAGCACCCCGGAGAATGAAGAGTGGAGCAGAAGCCAGCTGGTGTCCAGTGAGAAGAAGGACGGCTGG
  TCCTGGGAGTCCTACCTGGAGGAGCAGAAGGCCGTCACAGCCCCAGTTAGCCTCTTCCAGGACTCCCAGG
  CAGTCACCCATAACAAGAATGGCTTCAAACTGGGCATGAAGCTGGAAGGCATTGACCCCCAACACCCGTC
  AATGTACTTCATCCTCACTGTGGCTGAGGTGTGTGGCTACCGCCTGCGCCTGCACTTTGATGGCTACTCT
  GAGTGCCACGACTTCTGGGTCAATGCCAACTCCCCTGATATTCATCCCGCTGGCTGGTTTGAGAAGACTG
  GACACAAGCTGCAGCTTCCCAAAGGTTACAAGGAAGAGGAGTTCAGCTGGAGCCAGTACCTGCGCAGCAC
  GAAAGCTCAGGCAGCCCCCAAGCACCTGTTTGTAAGCCAGAGCCACAGTACTCCACCTGTGGGCTTCCAG
  GTGGGCATGAAGCTGGAAGCTGTGGACCGCATGAACCCTTCCCTCGTGTGTGTGGCCAGTGTGACCGATG
  TGGTGGACAGTCGCTTCCTGGTGCACTTTGATGACTGGGGCGATACTTACGACTACTGGTGTGATCCCAG
  CAGCCCCTACATCCACCCAGTAGGCTGGTGCCAGAAGCAAGGAAAGCCGCTCACCCCTCCACAAGACTAC
  CCAGACCCTGACAGCTTCTGCTGGGAGAAATATCTAGAAGAAACTGGGACCTCGGCTGTGCCCAATTGGG
  CCTTCAAAGTGCGACCCCCTCACAGCTTCCTGGTGAACATGAAGCTGGAGGCTGTAGACCGCCGGAACCC
  AGCACTGATCCGGGTAGCCAGTGTGGAAGATGTGGAGGATCATCGGATAAAGCTCCACTTTGATGGCTGG
  AGTCACAATTATGATTTCTGGATTGATGCCGATCACCCAGACATCCACCCTGCAGGCTGGTGCTCCAAGA
  CAGGACATCCCCTGGAGCCTCCTCTCCGGCCCAGAGAGTCCAGCTCTGTCTCCCCTGGGGGATGCCCCCC
  TCTCAGCCACAGGAGCCCACCCCACACAAAGACCTCCAAATATAACTTTCACCACCGAAAGTGCCCCACT
  CCTGGCTGTGACGGCTCCGGCCATGTCACTGGCAAGTTCACAGCTCACCATTGCCTCTCTGGCTGCCCGC
  TGGCCGAGAAAAACCAGAGCCGGCTGAAAGCGGAACTGTCCGACTCGGAGACCGCAGCCAGGAAGAAGAA
  CCCGTCTAATTTGTCCCCAAGGAAAAAGCCTCGCCATCAAGGCCGGATTGGACGCCCTCCAAAGTATCGC
  AAGATTCCAGAAGAAGATCTCCAAGCCCTCCCTCCCAGTGTGGTACACCAGTCCCTCTTCATGTCCACCT
  TGCCGACCCACGCTGATCGCCCACTCTCTGTGTGCTGGGAGCAACACTGCAAGCTGTTGCCAGGAGTGGC
  GGGCATCTCAGCCTCCACAGTCTCTAAGTGGACCATTGAAGAGGTCTTTGGCTTCGTTCAGACCTTGACG
  GGCTCTGAGGACCAAGCGCGCCTCTTCAAAGACGAGATGATTGACGGCGAGGCCTTCCTTTTGCTGACAC
  AGGCGGACATTGTGAAGATCATGAGTGTCAAGCTAGGCCCAGCCTTGAAGATCTATAACGCCATTCTCAT
  GTTCAAAAACACTGACGATGCCTTTAAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001081338
• Insert Size: 2481 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081338.1, NP_001074807.1
• RefSeq Size: 2733 bp
• RefSeq ORF: 2481 bp
• Locus ID: 241764
• UniProt ID: A2A5N8
• Cytogenetics: 2 H2
• Gene Summary: Polycomb group (PcG) protein that specifically recognizes and binds mono- and dimethyllysine residues on target proteins, therey acting as a 'reader' of a network of post-translational modifications. PcG proteins maintain the transcriptionally repressive state of genes: acts as a chromatin compaction factor by recognizing and binding mono- and dimethylated histone H1b/HIST1H1E at 'Lys-26' (H1bK26me1 and H1bK26me2) and histone H4 at 'Lys-20' (H4K20me1 and H4K20me2), leading to condense chromatin and repress transcription. Recognizes and binds p53/TP53 monomethylated at 'Lys-382', leading to repress p53/TP53-target genes. Also recognizes and binds RB1/RB monomethylated at 'Lys-860'. Participates in the ETV6-mediated repression. Probably plays a role in cell proliferation. Overexpression induces multinucleated cells, suggesting that it is required to accomplish normal mitosis (By similarity).[UniProtKB/Swiss-Prot Function]