Kdm1b (NM_172262) Mouse Untagged Clone

CAT#: MC221893

Kdm1b (untagged) - Mouse lysine (K)-specific demethylase 1B (Kdm1b), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Kdm1b
• Synonyms: 4632428N09Rik; AI482520; Aof1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221893 representing NM_172262
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGCATCTCGAGGGAGGTCAAAGAAGAGAAGCAATTTGGAGCTTTCTCCTGATAACCTGCCTTTGA
  GGAGCTCTGGGCGGCAGGCAAAGAAGAAAGCAGTAGAGATCCCGGATGAGGATGAAGATGGTTCGTCGGA
  GAAGAAGTACCGGAAATGTGAAAAGGCAGGCTGTACGGCGGCGTACCCTGTGTGCTTTGCAAGCGCTTCT
  GAAAGATGTGCCAAAAATGGTTACACCTCCCGATGGTATCACCTTTCCTGTGGGGAACACTTCTGCAATG
  AATGCTTTGACCACTACTACAGAAGCCACAAAGATGGGTATGACAAATACAGTGCATGGAAAAGAGTGTG
  GACCAGCAACGGCAAGACGGAGCCAAGCCCCAAGGCGTTCATGGCAGACCAGCAGCTGCCCTACTGGGTG
  CAGTGCACAAAACCCGAGTGTGGAAAGTGGAGGCAGCTGACCAAGGAGATCCAGCTTACTCCACACATGG
  CGAGGACTTACCGCTGTGGCATGAAGCCAAATACTATTACCAAGCCTGACACCCCCGATCATTGTTCTTT
  CCCCGAGGATCTGAGAGTGCTGGAAGTTTCCAACCACTGGTGGTACCCCATGCTCATCCAGCCTCCGTTG
  CTGAAGGACAGTGTGGCAGCACCGCTGCTTTCTGCCTACTACCCCGACTGCGTTGGCATGAGCCCCTCCT
  GCACCAGCACACATCGTGCCACCGTCACAGCTGCTACCACAACCACCGGCAGTGCCAGCCCGGGGGAGAT
  GGAGCCCTCCAAGGCAGCCCCCTCTTCACTTGTCCTAGGCATGAACCGGTACTTCCAGCCGTTCTACCAG
  CCCAACGAGTGTGGGAAAGCGCTGTGCGTGAGGCCAGACGTGATGGAGCTGGATGAGCTCTACGAGTTCC
  CAGAGTATTCGCGGGACCCCACCATGTACCTGGCTTTGAGAAACCTCATCCTCGCACTGTGGTACACAAA
  CTGCAAAGAAGCTCTCACCCCTCAGAAGTGCATTCCCCACATCATTGTCCGGGGCCTTGTCCGCATCAGA
  TGCGTTCAGGAAGTGGAGAGGATTCTTTACTTCATGACGAGGAAAGGCCTCATCAACACAGGCGTTCTCA
  CGGTGGCAGCCGGCCAGCATCTTCTTCCTAAACACTACCACAATAAATCTGTTCTGGTTGTTGGGGCTGG
  TCCAGCAGGCTTAGCAGCTGCTAGGCAACTGCATAACTTTGGGATGAAGGTGACTGTCCTGGAAGCCAAA
  GACCGGATTGGAGGCCGAGTATGGGATGACAAGTCCTTTAAAGGCGTGGTAGTAGGAAGAGGACCCCAGA
  TTGTCAATGGCTGTATTAATAACCCAGTAGCACTAATGTGTGAGCAACTTGGCATCAGCATGCGTAAGTT
  GGGAGAGAGATGTGACTTAATTCAGGAAGGTGGACGGATAACTGACCCCACTGTTGACAAACGCATGGAC
  TTTCATTTTAATGCTCTCTTGGATGTTGTCTCTGAGTGGAGGAAGGATAAGACTCTGCTCCAAGATGTCC
  CTTTAGGAGAGAAGATAGAGGAGATCTACCGAGCTTTTGTGAAGGAGTCTGGCATCCAGTTCAGCGAGCT
  GGAGGGACAGGTGCTGCAGTTCCACCTGAGTAACCTGGAGTATGCTTGTGGCAGCAGCCTGCACCAGGTG
  TCTGCTCGCTCCTGGGATCACAATGAATTCTTTGCCCAGTTTGCTGGTGACCATACCCTCCTCACACCAG
  GCTACTCCACTATCATTGAGAAACTGGCTGAAGGACTAGACATTCGACTCAAATCTCCAGTGCAAAGTAT
  AGATTATACTGGAGATGAAGTGCAGGTTACCACGACAGATGGTATGGGGCATTCTGCACAAAAGGTGTTA
  GTCACTGTGCCATTGGCCATACTACAGAGAGGTGCCATCCAGTTTAATCCACCATTGTCAGAAAAGAAGA
  TGAAAGCCATCAACAGCTTGGGTGCAGGCATCATTGAAAAGATTGCCTTGCAGTTTCCATATAGATTTTG
  GGACAGTAAAGTTCAAGGAGCTGACTTTTTTGGCCATGTTCCTCCCAGTGCCAGCCAACGAGGCCTCTTC
  GCTGTATTCTATGACATGGACTCTCAGCAGAGTGTGCTGATGTCGGTGATCACAGGGGAGGCAGTGGCGT
  CCCTCAGGACCATGGATGACAAGCAGGTGCTGCAGCAGTGCATGGGCATCCTTCGGGAGCTGTTCAAGGA
  GCAGGAGATCCCAGAACCCACAAAGTATTTTGTCACACGGTGGAGCACAGAGCCATGGATCCAGATGGCA
  TATAGTTTCGTGAAGACCTTTGGAAGTGGCGAGGCCTATGATATTATTGCTGAAGAAATACAAGGAACTG
  TCTTTTTTGCTGGTGAGGCAACAAACAGGCATTTCCCACAAACTGTTACTGGGGCATATTTAAGTGGTGT
  ACGAGAAGCAAGCAAGATTGCAGCCTTTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_172262
• Insert Size: 2481 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172262.3, NP_758466.1
• RefSeq Size: 4987 bp
• RefSeq ORF: 2481 bp
• Locus ID: 218214
• UniProt ID: Q8CIG3
• Cytogenetics: 13 A5
• Gene Summary: Histone demethylase that demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Required for de novo DNA methylation of a subset of imprinted genes during oogenesis. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Demethylates both mono- and di-methylated 'Lys-4' of histone H3. Has no effect on tri-methylated 'Lys-4', mono-, di- or tri-methylated 'Lys-9', mono-, di- or tri-methylated 'Lys-27', mono-, di- or tri-methylated 'Lys-36' of histone H3, or on mono-, di- or tri-methylated 'Lys-20' of histone H4.[UniProtKB/Swiss-Prot Function]