Lrguk (NM_028886) Mouse Untagged Clone

CAT#: MC221879

Lrguk (untagged) - Mouse leucine-rich repeats and guanylate kinase domain containing (Lrguk), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Lrguk
• Synonyms: 4921528H16Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221879 representing NM_028886
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCTTCGAGCGAAATCCCTCGAGATGGAAAGGCACCCGTTTCAGGCGGGGTTTGGGCGCCTCTC
  GCATTGCAGCCCAGGCGATACTGTCGCTCACAGAAAAACAAAGTCAGGGCCGTTGGCCTTCTTTCCCCCT
  GGGTCTGAAGTCAAAAGGGACTTTCAGAAGCGCCTCCTCTTACCTCCTCCACCAGCTCATCCACCGCTCT
  CATGAGGCAGAAGCGGAGCAAGAAGAGAAGCAACAGGAGGACGGAGAATCTGAAGAGTCAGAGGAGTCCG
  AAATGCAGAATTTGGAGGACAAGTATGACGGGATCCTGAGAGAGGAGACCGTGGCTGAGGCAATCACTGG
  ATTGGGGTGGTCAGGCCGTGGCACGGAGCAGGTTTACCTCAACCTGAATCTATCGCACTGTGAGTTGGTC
  GACATCAGCATTCTGTGTGGCTATGTCCACCTGCAGAAGTTGAATCTTTCGGGAAACAGAATCGAAGATT
  TGTCTTGTGTGAGCTGCATGCCGTATCTTCTAGAACTAAATGCTTCTCAGAACAAGCTGACTACCTTCTT
  CAATTTCAAACCACCCCAAAATCTCAAGAAGGTGGATTTTTCCTCCAACCTGATTTCCGAGATGTATGAC
  CTGTCAGCGTACCATACTCTCACTCAACTAATTTTGGACAACAACGAGATAGAAGAAATCACTGGGCTAG
  AGAACTGCATCAGCCTGACCCACCTTAGTCTGGCTGGCAACAAGATCACTACCATCAAAGGCTTAGGGAC
  ATTACCCATCAAAGTGCTTTCTTTGAGTAACAACATGATTGAGACCATCACAGGTCTGGAGGAGCTGAAA
  GCCTTGCAGAACCTGGACCTGTCCCACAATCAGATCAGCAGCCTCCAGGGCTTGGAGAACCATGACCTCC
  TGGAAGTGATTAACCTGGAGGATAACAAGATTAAGGAGCTGAGTGAAATAGAATACATTGAAAATCTTCC
  TATCCTTCGAGTTCTCAATCTACTAAGGAATCCAATTCAGACCAAGCCCGAGTACTGGTTCTTCGTGATA
  TATATGCTGCTGCGACTGACAGAGTTAGACCAGCAGAAGATTAAGGTAGAAGAAAAGGTTTTTGCAGTGA
  ATAAGTATGACCCTCCCCCTGAGGTGGTCGCTGTCCAAGATCACATGACCCATGTTGTCAACAGCATGTC
  GCAGCCACAGAGGATCTGGGACAGCACCCTGCCGAGTCTGGATGCCCCGTATCCCATGCTCATACTAACT
  GGGCCTGCAGCTTGCGGGAAACGAGAACTCGCCCACCGCCTCTGCAGACAGTTTAGTACTTACTTCAGAT
  ATGGGGCCTGTCACACCACGAGACCGCCTTACTTTGGTGAAGGAGATCGAGTTGATTATCATTTCATTTC
  CCAAGAAGTTTTTGACGAAATGCTGAACATGGGGAAATTTATTTTAACATTTAATTATGGCAATCACAAT
  TATGGATTAAATAGGGACACGATAGAAGGTATTGCAAGAGATGGATTAGCAAGCTGCATTCATATGGAAT
  TAGAAGGTGTAAGAAGTTTGAAATATTCTTATTTTGAGCCTCGATATATCTTGGTGGTGCCTATGGACAA
  GGAAAAATATGAGGGATACTTGCGGAGAAAGGGATTGTTCAGTCGCGCAGAAATCGAAATTGCCGTCTCC
  AGGGTGGACCTTTATGTTAAAGTTAATCAGAAATACCCAGGATATTTTGATGCAGTAATCAATGCAGATG
  ACATGGACATTGCCTACCAAAAGCTCAGCGAGCTCATTAGAGAATACCTGGGGTTGACTGAGACAGCTGC
  TAAGACCTTGGCTCCAACTGCAGATACTAAGACGTCTTACCTGAAATGCGAAGACTATTCTAGGAAGTCC
  AGTACAGTTGAATTCTTGGATTCAACAGACAGAAACTACTTTACCAAATTATGGGCCAAACTTTCATCCA
  AAAAGTCACCAGTGGAAAGAGAGTCTCTCCACAGACAGCATGAGGCAGCCCGGCAGGCGCTCATGGGGAA
  GACGCCCCGTGATCACACGCTCCTGTTTCAAAGGGGTCCAGTACCGATACCTACTGTCAGTGGCCAACAG
  TATTTTGCAACAATAGATGAACTACAGAAAACGTTTGAGCTCTCTGACGATCTCTTTAAAACCCCCTCTG
  GAACATACCCTGAAACAAGCAAGGATTCCAATATTTCTAAGAGATATTCTACATACTTTCATACTTGTCC
  ATGGTCCAAAGAACTTCCCTTTCAGCTTCCAGAGGGTGGGATCTCATCGCGCCCAGGGTCAGCAGGGAGT
  GACGAAGTCGATGGGGCATTGAAAGCTCTTCGTGTAGCATCATCTATGCAGGAAAAAGTGGCCCAGCACA
  AAAGACTGTCTGCGATTACAATAATGGACCCGGGTTCCAACACCAAACCCACCCTTCCTCCGATCCCGCA
  CGGCCGCAGATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_028886
• Insert Size: 2463 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028886.1, NP_083162.1
• RefSeq Size: 3058 bp
• RefSeq ORF: 2463 bp
• Locus ID: 74354
• UniProt ID: Q9D5S7
• Cytogenetics: 6 A3.3
• Gene Summary: Involved in multiple aspects of sperm assembly including acrosome attachment, shaping of the sperm head and in the early aspects of axoneme development (PubMed:25781171). Not essential for primary cilium biogenesis (PubMed:28003339).[UniProtKB/Swiss-Prot Function]