Pkp3 (NM_001162924) Mouse Untagged Clone

CAT#: MC221872

Pkp3 (untagged) - Mouse plakophilin 3 (Pkp3), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pkp3
• Synonyms: 2310056L12Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221872 representing NM_001162924
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCCACCGCAGGATCTCGGACCCGGATGGAGCCCAGAAGGAATTGTCCTACAGCAGGGACCTCCA
  GAATGAGCCAGGGAGCCAGTGGGGGCCAGACCAGCGGCAAGCCTGAGACTGGTGTTTGCTCCCTGGCTCT
  GCCTTCGGACCTACAGCTGGATCGCCGGGGTGCGGAGGGACCGGAGGCTGATCGACTTAGAGCTGCCAGA
  GTCCAGGAGCAAGTCCGAGCCCGCCTCCTGCAGCTGGGCCAGCAGTCGCGGCACAATGGGTCTGCTGAGC
  TTGATGGCTCCGCGGAGAGCGCCAGAGGCATGCCCAGAGGTCAATATCACACTATGCAGACTGGCTTCAG
  CTCCCGATCCCAGGGCATGAGTGGAGACAAGACCTCGACCTTCCGGCCCATCGCCAAGCCGGCCTACAGC
  CCAGCTTCCTGGTCCTCCCGCTCAGCCGTGGATCTGACGTGCAGTCGGAGGCTGAGTTCTGCCCACAATG
  GGGGCAGTGCCTTTGGGGCTGTGGGATATGGGGGTACCCAGCCCACTCCACCTATGCCCACCCGGCCAGT
  GTCCTTCCATGAGCGAGGTGGGGCAGCCAGCAGAGCTGACTATGACACATTATCCCTGCGCTCACTGAGG
  CTGGGGCCAGGGGGCCTGGATGACCGTTACAGCGTGGTATCTGAGCAACTGGAGCCTGCAGCCGCCTCTA
  CCTACAGGGCCTATGCTTACGAGCGCCAGGCTAGCTCCGGCTCTAGCCGAGCAGGGGGCCTGGACTGGCC
  AGAGGCCACAGAGGGACCACCCAGCAGGACCATCCGTGCACCTGCCATGCGAACCCTGCAAAGATTTCAG
  AGCAGCCACCGAAGCCGTGGGGGTACCGGGTCAGTGTCAGGTGCCGGCTTGGAGCCAGTGGCCCGAGCCC
  CATCTGTGCGCAGCCTCAGTCTTAGCCTGGCTGACTCAGGCCACCTGCCAGATGTACGCGGGCTGGACAG
  CTACACGGGCCATCGAACCCTGCAGAGGCTCAGCAGTGGCTTTGATGACATTGACCTGCCTTCAGCAGTC
  AAGTACCTCATGGCCTCAGACCCCAACCTGCAGGTGCTGGGAGCAGCTTACATCCAGCACAGGTGTTACA
  GTGATGCCGCCGCCAAGAAGCAGGCTCGCAGCCTTCAGGCAGTGCCTAGACTGGTGAAGCTCTTCAACCA
  CGCCAACCAGGAGGTGCAGCGCCACGCCACAGGTGCCATGCGCAACCTCATCTACGACAATGTAGACAAC
  AAGCTGGCGTTGGTAGAGGAGAATGGCATTTTTGAGCTGTTGCGGACACTTCGAGAGCAGGATGATGAGC
  TACGCAAAAATGTTACAGGGATCCTATGGAACCTGTCCTCCAGTGACCACCTAAAGGACCGCCTCGCCCG
  GGATACACTGGAGCAGCTCACAGATCTGGTGCTGAGCCCCCTGTCAGGGGCAGGTGGGCCTCCCCTCATC
  CAGCAGAATGCCTCAGAGGCAGAGATCTTCTACAATGCTACTGGCTTCCTCAGGAACCTCAGCTCAGCCT
  CCCAGGCCACTCGGCAGAAGATGCGCGAGTGCCATGGGCTAGTGGATGCTCTGGTCACCTACATCAACCA
  TGCCCTGGACGTTGGCAAGTGTGAGGACAAGAGTGTGGAAAATGCTGTGTGTGTGCTGAGGAACCTGTCC
  TACCGCCTCTACGACGAGATGCCGCCATCAGCCTTGCAGCGGCTTGAAGGCCGGGGCCGCAGGGACATGG
  CAGGGGCTCCGCCTGGTGAAATGGTAGGCTGCTTCACACCTCAGAGTCGGCGGCTGCGAGAGCTTCCTCT
  CACAGCTGATGCACTCACCTTTGCCGAAGTATCTAAGGATCCCAAGGGCCTCGAATGGCTGTGGAGCCCC
  CAGATTGTGGGGTTGTACAACCGACTGCTGCAGCGCTGTGAACTAAATCGGCATACAACGGAGGCGGCCG
  CAGGGGCGCTACAGAATATCACCGCGGGCGACCGCAGGTGGGCAGGTGTGCTGAGCCGCCTGGCCCTGGA
  ACAGGAGCGAATCCTGAACCCCTTGCTGGACCGCGTTCGCACCGCTGACCACAACCAACTGCGCTCACTG
  ACTGGCCTCATCCGAAACCTGTCTCGGAATGCCAGGAACAAGGACGAGATGTCCACAAAGGTAGTGAGCC
  ACCTGATTGAGAAGCTTCCTGGTAGTGTGGGTGAGAAGTGTCCCCCAGCTGAGGTGCTGGTCAACATCAT
  AGCCGTGCTCAACAACCTGGTGGTAGCCAGCCCCATTGCTGCCCGGGACCTGCTCTACTTTGATGGCCTC
  CGAAAGCTGGTCTTCATCAAAAAGAAGAGGGATAGCCCCGACAGTGAGAAGTCTTCCCGGGCAGCCTCCA
  GCCTCTTGGCCAACCTCTGGCAGTACAGCAAGCTCCACCGTGACTTCCGGGCGAAAGGCTACCGGAAAGA
  GGACTTCCTGGGTCCATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001162924
• Insert Size: 2469 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001162924.1, NP_001156396.1
• RefSeq Size: 2926 bp
• RefSeq ORF: 2469 bp
• Locus ID: 56460
• UniProt ID: Q9QY23
• Cytogenetics: 7 F5
• Gene Summary: May play a role in junctional plaques.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate exon for its 5' UTR and 5' coding region, compared to variant 1, that results in a protein (isoform 2) with a longer and distinct N-terminus, compared to isoform 1.