Pde8a (NM_008803) Mouse Untagged Clone

CAT#: MC221860

Pde8a (untagged) - Mouse phosphodiesterase 8A (Pde8a), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pde8a
• Synonyms: AI551852; Pde8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221860 representing NM_008803
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCTGCGCCCCGAGCATCCACACTTCCGAAAACCGAACGTTTAGCCACAGCGACGGCGAGGACGAGG
  ACGTGGACGTGGACGTCCCGGGCCCCGCGCCGAGATCTATTCAGCGATGGTCGACCGCGCCCGGGCTCGT
  GGAGCCTCAGCCTCGGGATAACGGCGCCTCGAAGGTATCAGTAGCTGATGTGCAATTTGGCCCGATGAGA
  TTTCATCAAGACCAACTTCAGGTCCTGTTAGTGTTTACCAAAGAAGATAGCCAGTGTAACGGATTCCACA
  GGGCATGTGAGAAAGCTGGGTTTAAGTGCACAGTCACCAAGGAGGTGCAGACTGTCCTTACCTGTTTCCA
  GGACAAACTCCATGACATCATCATCATAGACCACAGATATCCCCGACAGATGGATGCAGAGACATTGTGC
  AGGTCTATCAGATCATCAAAATTCTCAGAAAACACAGTTATTGTTGGCGTAGTACGCAGGGTGGACAAAG
  AAGAATCATCTTTAATGCCCTTCCTTGCTGCTGGCTTCACACGGAGGTTTATAGAGAACCCCAACGTCAT
  GGCCTGTTATAATGAACTGCTCCAGTTGGCATGTGGAGAGGTGCGATCACAGCTGAAACTCAGGGCTTGT
  AACTCAGTATTCACTGCATTAGAGAAAAGTCAAGAAGCAATTGAAATTACAAGTGAAGACCACATTATAC
  AGTATGCAAACCCTGCATTTGAATCAACAATGGGTTATCAGTCAGGAGAATTAATAGGGAAGGAGTTAGC
  ACAAGTGCCCATAAATGAAAAGAAAGGTGATTTGCTCGATGCCATAAATTCGTGTGTCACAGTAGACAAG
  GAATGGCAAGGAGTTTACCATACCCAAAAGAAAAATGGAGATAACATACAGCAAAATGTGAAGATAATAC
  CTGTCATTGGGCAGGGAGGAAAAATCAGACACTATGTGTCCATTATCAGAGTGTGCAATGGCAACAATAA
  GGTTGAGACCACAACTGAGTGTGTTCAGACTGACAGTCAAACAGATAACCAGGCAGGCAAACATAAGGAC
  AGGAGGAAACATTCGATGGACGCCAAAGCGGTTTCCTCCAGAACAAGTGACGTTTCCAGTCAGAGAAGAC
  ATTCCTCTTTGGCCCGGATACATTCTATGATGATTGAGGCACCCATCACCAAGGTAATCAATATTATCAA
  TGCTGCCCAGGAAAACAGTCCTGTGCCTGTGACGGAAGCCTTGAACCGAGTGCTGGATATTCTGAGAACC
  ACTGAGCTGTACTCGCCACAGTTTAATGCTCAGGATGATCCCCATGCCACAGACCTCGTTGGGGGCTTAA
  TGTCTGATGGCTTACGAAGGTTTTCAGGAAATGAGTATATTCTTGCAACAAAAAACCTCCCACCACTTTC
  AAACAATTTGGCCACCCCCGTCTCCCTTCATGATGTCCCACCACGGATTGCCCTGGCCATAGAAAATGAA
  GAACAGTGGGACTTTGACATTTTTGAGCTGGAAGTAGCAACCCAAAATAGGCCTTTGATTTATCTAGGTC
  TGAAAACGTTTGCGCGTTTTGGAATGTGTGAGTTCTTACAGTGCTCTGAGACAACGCTGAGATCTTGGTT
  TCAGATGATCGAATCCAACTACCACTCTTCTAACCCCTACCACAATTCCACACACGCTGCGGATGTGCTT
  CACGCCACGGCGTATTTCCTTTCCAGAGACAAAATAAAGGAAACTTTAGATCGCATTGACGAGGTTGCTG
  CCCTCATTGCGGCCACCGTCCATGACGTGGATCACCCTGGGAGAACCAACTCCTTCCTGTGCAACGCGGG
  AAACCAGCTGGCTGTGCTCTACAACGACACCGCTGTGCTGGAGAGCCACCACGTGGCACTGGCCTTCCAG
  CTGACGCTTGAAAATGACCAGTGCAATATCTTTAAGCAAATGGAGAGGAATGACTACCGGACACTACGCC
  AGAGCATTATTGACATGGTCCTAGCCACAGAAATGACGAAGCACTTTGAACATGTCAACAAATTTATCAA
  CAGCATCAACAAGCCTTTGACAGCCCAGGAGAGTGAGGAACCTGACAGAAGTCTGGAAGACATAAAAGCT
  ATGCTCAAGACGCCAGAGAGCCGGGCTCTCATCAAAAGAATGATGATTAAGTGTGCCGACGTGTCCAACC
  CCTGCCGGCCGCTGGAGCACTGCATCGAGTGGGCTGCGCGCATCTCAGAGGAGTATTTCTCTCAGACTGA
  TGAAGAGAAACAGCTGGACTTGCCTGTGGTGATGCCCGTGTTTGACAGAAACACCTGCAGCATTCCCAAG
  TCTCAGATCTCCTTCATCGATTACTTCATCACAGACATGTTTGATGCCTGGGATGCCTTCGTTGACCTGC
  CTAACCTTATGCAGCACCTAGATGACAACTTCAGGTACTGGAAAGGACTGGATGAGAAGAAGCTTCGAAG
  CCTCCGCCCCCCTCCAGAGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008803
• Insert Size: 2472 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008803.2, NP_032829.1
• RefSeq Size: 2683 bp
• RefSeq ORF: 2472 bp
• Locus ID: 18584
• UniProt ID: O88502
• Cytogenetics: 7 D3
• Gene Summary: Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in maintaining basal levels of the cyclic nucleotide and/or in the cAMP regulation of germ cell development. Binding to RAF1 reduces RAF1 'Ser-259' inhibitory-phosphorylation and stimulates RAF1-dependent EGF-activated ERK-signaling. Protects against cell death induced by hydrogen peroxide and staurosporine.[UniProtKB/Swiss-Prot Function]