Hells (NM_008234) Mouse Untagged Clone

CAT#: MC221853

Hells (untagged) - Mouse helicase, lymphoid specific (Hells), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Hells
• Synonyms: AI323785; E130115I21Rik; LSH; Lysh; PASG; YFK8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221853 representing NM_008234
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGAACAAACGGAGCCTGCGGTGATTACTCCAGCCATGCTGGAGGAGGAGGAACAGCTCGAGGCTG
  CGGGACTTGAGAAAGAGCGGAAGATGTTAGAAGAGGCTCAAAAGTCTTGGGACAGAGAGTCCACTGAAAT
  TCGGTACCGCAGACTTCAGCATTTGCTTGAAAAAAGCAATATCTACTCAAAATTTTTGTTGACTAAAATG
  GAACAACAGCAATTGGAGGAACAGAAGAAGAAAGAGAAGTTGGAGAAAAAAAAACGGTCATTAAAACTTA
  CAGAGGGTAAAAGTTTAGTTGATGGGAATGGAGAGAAACCAGTCATGAAAAAGAAAAGAGGAAGAGAAGA
  TGAATCTTATAATATTTCAGAGGTCATGTCAAAAGAGGAAATTTTGTCCGTAGCTAAAAAACATAAGGAT
  AATGAGGATGAAAGCTCTTCCACTACGAGTCTTTGTGTAGAAGATATTCAGAAAAATAAGGATTCAAATA
  GTATGATTAAAGATAGATTGTCTCAAACTGTTAGGCAGAATTCTAAATTCTTTTTTGACCCAGTTCGGAA
  ATGTAACGGACAGCCTGTACCCTTTCAACAACCAAAGCATTTCACAGGAGGAGTAATGAGGTGGTACCAA
  GTAGAAGGCATGGAATGGCTTAGGATGCTTTGGGAAAATGGAATTAATGGCATTTTAGCAGATGAAATGG
  GTTTGGGAAAGACTGTTCAGTGCATTGCTACAATTGCATTGATGATTCAGAGGGGAGTACCTGGACCTTT
  TCTTGTTTGTGGCCCTTTGTCTACACTTCCTAACTGGATGGCTGAATTTAAAAGATTTACTCCAGAAATT
  CCTACTCTGCTGTATCATGGAACCCGGGAGGACCGTCGGAAATTAGTAAAGAATATCCACAAAAGACAAG
  GGACACTGCAGATTCATCCTGTGGTGGTCACATCATTCGAGATCGCTATGCGAGACCAGAATGCTTTACA
  GCATTGCTATTGGAAATACTTAATAGTAGATGAAGGACACAGGATTAAGAATATGAAGTGCCGGCTAATC
  AGGGAGTTAAAACGGTTCAATGCTGATAACAAACTTCTTTTGACTGGTACTCCCTTGCAAAACAATTTAT
  CAGAACTTTGGTCATTGCTAAACTTTTTGTTGCCAGATGTATTTGATGACTTGAAAAGCTTTGAGTCTTG
  GTTTGATATCACTAGTCTTTCTGAAACTGCTGAAGATATTATAGCTAAAGAGAGAGAACAGAATGTTTTA
  CATATGCTGCACCAGATTTTAACGCCTTTCCTACTGAGAAGACTGAAGTCTGATGTTGCTCTTGAAGTCC
  CCCCTAAGCGAGAAGTAGTTGTTTATGCACCACTTTGCAATAAACAAGAGATCTTCTATACAGCTATTGT
  GAACCGCACAATTGCAAACATGTTTGGATCCTGTGAGAAGGAAACAGTTGAGCTAAGTCCTACTGGAAGA
  CCAAAACGACGAAGTAGAAAGTCAATAAATTACAGCGAACTAGATCAGTTCCCTAGTGAATTAGAAAAAC
  TAATAAGTCAAATACAGCCAGAAGTAAACAGAGAGAGGACTGTTGTGGAAGGTAATATCCCTATAGAATC
  TGAAGTTAATCTGAAGCTGCGGAATATAATGATGCTACTTCGAAAGTGCTGTAATCACCCATATATGATT
  GAATATCCTATTGACCCTGTCACACAAGAATTTAAGATTGATGAAGAGTTGGTAACAAATTCTGGGAAGT
  TCTTAATTTTGGATCGAATGCTGCCCGAACTTAAAAAAAGAGGTCACAAGGTTCTGGTGTTTTCACAAAT
  GACAAGCATGTTGGACATCTTGATGGATTATTGCCATCTTAGAAACTTCATCTTTAGCAGACTTGATGGG
  TCCATGTCTTATTCGGAAAGAGAAAAAAATATATACAGTTTCAACACGGATCCAGATGTTTTTCTTTTCT
  TAGTGAGTACACGAGCTGGTGGTTTAGGCATAAACTTGACTGCAGCAGATACAGTGATCATTTATGACAG
  TGATTGGAATCCTCAGTCTGATCTCCAGGCTCAAGATAGATGTCATAGAATTGGTCAGACAAAGCCAGTT
  GTTGTGTATCGTCTTGTAACAGCAAATACTATTGACCAGAAAATTGTGGAAAGAGCAGCTGCTAAAAGAA
  AGTTGGAGAAGTTGATAATCCATAAAAATCATTTCAAAGGTGGTCAGTCTGGGTTAAGTCAATCTAAGAA
  TTTCTTAGATGCAAAGGAATTAATGGAGTTATTAAAATCTAGAGATTATGAAAGGGAAGTAAAAGGATCC
  AGAGAGAAGGTCATTAGTGACGAAGATCTAGAATTACTGTTGGATCGAAGTGATCTCATTGATCAAATGA
  AGGCTTCAAGACCAATTAAAGGGAAGACAGGGATATTCAAAATACTAGAAAATTCTGAAGATTCCAGTGC
  TGAATGTTTATTTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008234
• Insert Size: 2466 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008234.3, NP_032260.2
• RefSeq Size: 3039 bp
• RefSeq ORF: 2466 bp
• Locus ID: 15201
• UniProt ID: Q60848
• Cytogenetics: 19 C3
• Gene Summary: Plays an essential role in normal development and survival. Involved in regulation of the expansion or survival of lymphoid cells. Required for de novo or maintenance DNA methylation. May control silencing of the imprinted CDKN1C gene through DNA methylation. May play a role in formation and organization of heterochromatin, implying a functional role in the regulation of transcription and mitosis.[UniProtKB/Swiss-Prot Function]