Mcm6 (NM_008567) Mouse Untagged Clone

CAT#: MC221835

Mcm6 (untagged) - Mouse minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe) (S. cerevisiae) (Mcm6), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mcm6
• Synonyms: ASP-l1; D1Wsu22e; Mcmd6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221835 representing NM_008567
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCTCGCAGCGGCCGCGGAGCCCGGCGCCGGAAGCCAGCACCCGGAGGTGCGCGACGAGGTGGCCG
  AGAAATGCCAGAAGCTGTTCCTAGACTTCCTGGAAGAGTTCCAGGGTAGTGATGGAGAAATTAAATACTT
  GCAGTTTGCAGAGGAGCTAATTCGTCCTGAGAGAAACACGCTGGTTGTGAGTTTTGCAGACCTGGAACAA
  TTTAACCAACAACTTTCTACCACCATTCAGGAAGAGTTCTATAGAGTCTACCCTTACCTGTGTCGAGCCT
  TGAAGACCTTTGTCAAAGACCGAAAGGAGATCCCTTTTGCTAAGGATTTTTATGTTGCATTCCAAGACCT
  ACCTACTAGACACAAGATTCGTGAGCTCACTTCATCCAGGATTGGGCTGCTCACCCGAATCAGTGGCCAG
  GTGGTGCGCACTCACCCAGTTCACCCTGAGCTTGTGAGTGGAACTTTCCTGTGCCTTGACTGTCAAACAG
  TGATTAAGGACGTAGAGCAGCAGTTCAAATACACACAGCCGAATATCTGCCGAAATCCAGTGTGTGCCAA
  CAGGAAGAGGTTCCTTCTAGACACTAATAAATCAAGATTTGTTGATTTTCAAAAGGTTCGCATTCAAGAG
  ACTCAAGCGGAACTTCCCCGGGGAAGTATTCCCCGGAGTTTAGAGGTTATCCTGAGAGCTGAAGCTGTGG
  AGTCAGCCCAAGCTGGTGACAGATGTGATTTCACAGGGGCACTGATTGTTGTGCCTGATGTCTCCAAGCT
  TAGCACACCAGGAGCACGTGCAGAGACTAACTCCCGAGTCAGTGGAGCTGATGGGTATGAGACAGAAGGC
  ATTCGAGGGCTCCGGGCCCTTGGTGTCAGAGATCTGTCATACAGGCTGGTCTTCCTTGCCTGCCATGTTG
  CACCAACCAACCCAAGGTTTGGAGGGAAAGAACTCAGAGATGAGGAACAGACCGCTGAGAGCATTAAGAA
  CCAAATGACGGTGAAGGAATGGGAAAAGGTGTTTGAAATGAGTCAGGACAAAAACCTGTACCACAATCTC
  TGCACGAGCCTCTTCCCTACTATACATGGCAATGATGAAGTAAAACGCGGTGTCTTACTGATGTTGTTCG
  GTGGTGTTCCTAAGACAACAGGGGAAGGAACCTCTCTTCGTGGGGATATAAATGTTTGCATTGTTGGTGA
  TCCAAGTACAGCTAAGAGCCAATTTCTCAAGCACGTGGACGAGTTCAGTCCCAGAGCTGTCTATACCAGT
  GGCAAAGCCTCCAGCGCTGCCGGCTTAACAGCAGCTGTGGTGAGAGATGAAGAATCTCACGAATTTGTCA
  TTGAGGCTGGAGCGTTGATGCTGGCTGATAATGGTGTCTGTTGTATTGATGAATTTGATAAGATGGACAT
  GCGGGACCAAGTTGCTATTCACGAAGCTATGGAGCAGCAGACCATCTCTATCACGAAAGCAGGAGTGAAG
  GCAACTCTGAATGCCAGGACATCCATTTTAGCTGCAGCAAACCCTGTCAGCGGACACTATGACAGATCAA
  AATCTTTGAAACAGAACATCAATCTGTCAGCGCCCATCATGTCCCGCTTTGATCTCTTCTTCATTCTGGT
  GGATGAATGCAATGAGGTGACAGATTATGCTATTGCCAGGCGCATAGTAGACTTGCATTCAAGAATTGAA
  GAATCGATCGATCGAGTTTATTCCCTTGATGATATAAGAAGGTACCTTCTCTTTGCCCGACAGTTTAAAC
  CCAAGATTTCCAAAGAGTCAGAAGACTTCATTGTGGAGCAGTACAAACGTCTCCGCCAGAGGGATGGCTC
  TGGGGTCACCAAGTCCTCGTGGAGAATCACCGTGCGACAGCTTGAGAGCATGATCCGACTCTCAGAATCA
  ATGGCCCGCATGCACTGCTGTGACGAGGTTCAGCCTAAACATGTGAAGGAAGCTTTTCGATTACTGAATA
  AATCAATCATCCGTGTAGAGACGCCTGATGTCAATCTAGATCAAGAGGAAGAGATCCAGATGGAGACGGA
  TGAGGGACAAGGAGGCGTCAATGGTCATGCCGACAGCCCTGCCCCTGTGAATAGGTTCAACGGCTCCAGT
  GAAGATGCCAGCCAGGAGACTGTTTCCAAGCCCTCCCTGAGGCTGGGCTTTGCTGAATACTGCCGAATCT
  CGAACCTCATCGTGCTCCACCTCAGGAAAATGGAAGAAGAGGAGGACGAGTCGGCACTAAAGAGAAGCGA
  GCTTGTCAACTGGTACCTGAAGGAAATCGAATCAGAGATAGACTCTGAAGAAGAGCTCATAAATAAAAAG
  ACGATCATTGAGAAGGTTGTTCATCGTCTCACACACTACGATCACGTTCTGATTGAGCTCACCCAGGCTG
  GGCTGAAAGGCTCCAGTGAAGGAAGCGAGAGCTATGAGGAAGACCCCTACCTGGTAGTCAACCCTAACTA
  CTTGCTTGAAGATTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008567
• Insert Size: 2466 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008567.2, NP_032593.1
• RefSeq Size: 2966 bp
• RefSeq ORF: 2466 bp
• Locus ID: 17219
• UniProt ID: P97311
• Cytogenetics: 1 55.8 cM
• Gene Summary: Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longer isoform (1).