Eml1 (NM_001043335) Mouse Untagged Clone

CAT#: MC221810

Eml1 (untagged) - Mouse echinoderm microtubule associated protein like 1 (Eml1), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Eml1
• Synonyms: 1110008N23Rik; A930030P13Rik; AA171013; AI847476; AI853955; ELP79; EMAP; EMAPL; heco
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221810 representing NM_001043335
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGACGGCTTCTCCAGCTATAGCAGCCTGTACGACACGTCCTCGCTGCTGCAGTTCTGCAACGATG
  ACAGCGCGTCTGCTGCCAGCAGCATGGAGGTGTCAGACCGCATCGCCTCTCTGGAGCAGCGCGTCCAGAT
  GCAGGAGGATGACATTCAACTGCTCAAGTCAGCGCTGGCCGACGTGGTTCGGAGGCTGAACATCACGGAG
  GAGCAACAGGCTGTGCTCAACAGGAAAGGACCTACCAAAGCGAGGCCACTGGGGCAGACCCTGCCATTGA
  GAACCACCGTCAACAATGGCACCGTGTTACCAAAGAAACCCAGTGCCTCCCTCCCGGCACCCTCCGGGGC
  CAGGAAAGAAGTAGTTGTGCCGGTAACCAAAAGCATCAATAGGACCAGCTCTTCCGAAAGAGTGTCTCCA
  GGTGGCCGGAGGGAGAGCTCTGGGGACTCCAAAGGAAGCCGGAACCGCACGGGCTCAACCAGTAGCTCCT
  CCAGCGGCAAGAAGAACAGTGAGAGCAAACCCAAGGAGCCCGCATTCAGTCCAGAAGAAGGATATGTAAA
  AATGTTTCTTCGAGGCCGTCCGGTCACCATGTACATGCCCAAAGACCAAGTGGATTCGTACAGTTTGGAA
  GCAAAAGCTGAGTTACCAACGAAGCGGCTGAAACTGGAGTGGGTCTACGGGTACCGGGGGCGAGACTGTC
  GCAATAACTTGTACTTGCTCCCGACCGGGGAGACCGTGTACTTCATTGCGTCTGTCGTGGTGCTTTACAA
  TGTGGAGGAGCAGCTGCAGAGGCATTACGCGGGCCACAACGATGACGTCAAGTGCCTAGCGGTCCATCCT
  GACAGGATCACCATAGCAACGGGACAAGTGGCGGGCACATCTAAGGATGGAAAGCAACTGCCACCACATG
  TGCGCATCTGGGACTCTGTGACACTGAACACTCTGCATGTCATTGGAATAGGCTTTTTTGACCGGGCTGT
  CACCTGCATCGCATTCTCAAAGTCTAACGGAGGAGGCCATCTCTGTGCTGTGGATGATTCCAACGATCAC
  GTGCTGTCCGTGTGGGATTGGCAGAAAGAAGAGAGACTGGCCGACGTGAAGTGTTCGAACGAAGCGGTAT
  TTGCGGCGGACTTCCACCCCACAGACACCAATATCATAGTCACCTGCGGGAAGTCACATCTCTACTTTTG
  GACCCTAGAAGGAAATTCCCTTAACAAGAAGCAAGGGTTGTTTGAGAAACAAGAGAAGCCAAAGTTTGTT
  CTCTGCGTGACGTTTTCTGAAAATGGCGACACCATTACTGGAGATTCAAGCGGCAACATCTTGGTGTGGG
  GGAAAGGTACAAATCGGATAAGCTATGCAGTTCAAGGGGCCCACGAGGGCGGCATTTTTGCACTTTGTAT
  GCTGAGAGACGGGACGCTGGTGTCCGGAGGAGGGAAGGACCGGCGACTCATTTCCTGGAACGGAAACTAT
  CAAAAACTTCACAAAGCAGAGATTCCTGAGCAGTTTGGCCCAATACGGACAGTAGCCGAAGGGAAGGGCA
  ACGTCATCTTGATAGGCACTACTAGAAACTTTGTCCTGCAAGGCACCTTGTCAGGGGACTTCACACCCAT
  CACTCAGGGCCACACCGATGAGCTCTGGGGGCTAGCCATCCATGCCTCCAAGCCTCAGTTCCTGACTTGT
  GGACATGACAAACATGCCACTCTCTGGGACGCTGTCGGTCACCGGCCAGTCTGGGACAAAATCATAGAGG
  ATCCAGCTCAGTCCTCTGGTTTTCATCCTTCCGGGTCTGTGGTTGCAGTGGGGACACTGACTGGGAGGTG
  GTTTGTGTTTGACACGGAGACAAAGGACTTGGTCACCGTCCACACGGATGGGAATGAGCAGCTGTCCGTG
  ATGCGGTATTCTCCAGATGGGAACTTCTTAGCAATCGGCTCCCATGACAACTGCATCTACATATATGGAG
  TTACCGACAATGGAAGGAAGTACACACGAGTTGGCAAGTGCTCCGGCCATTCCAGCTTCATCACCCACTT
  GGACTGGTCCGTGAACTCACAATTCCTGGTGTCAAATTCCGGGGACTACGAGATCCTCTACTGGGTTCCG
  TCTGCCTGTAAGCAAGTCGTGAGTGTGGAAACCACAAGGGACATCGAGTGGGCCACCTATACCTGCACCT
  TGGGATTCCACGTCTTTGGAGTGTGGCCGGAGGGCTCCGATGGGACAGACATCAACGCCGTCTGCCGGGC
  TCACGAGAGAAAGCTCTTGTGCACAGGCGATGACTTCGGCAAAGTGCACCTCTTCTCATACCCGTGCTCA
  CAGTTCCGGGCTCCAAGCCACATCTACAGTGGACACAGCAGCCACGTCACCAACGTGGACTTCCTCTGTG
  AAGACAGCCACCTTATCTCCACGGGTGGGAAAGACACAAGCATCATGCAGTGGCGAGTCATTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001043335
• Insert Size: 2445 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001043335.1, NP_001036800.1
• RefSeq Size: 4160 bp
• RefSeq ORF: 2445 bp
• Locus ID: 68519
• UniProt ID: Q05BC3
• Cytogenetics: 12 59.46 cM
• Gene Summary: Modulates the assembly and organization of the microtubule cytoskeleton, and probably plays a role in regulating the orientation of the mitotic spindle and the orientation of the plane of cell division. Required for normal proliferation of neuronal progenitor cells in the developing brain and for normal brain development. Does not affect neuron migration per se.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longest isoform (1).