Rabgap1l (NM_013862) Mouse Untagged Clone

CAT#: MC221806

Rabgap1l (untagged) - Mouse RAB GTPase activating protein 1-like (Rabgap1l), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rabgap1l
• Synonyms: 5830411O09Rik; 8430421H08Rik; 9630005B12Rik; AW049894; Hh1; HHL; mKIAA0471
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221806 representing NM_013862
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGTCAGAGCTTCGTTCCAGAAGGTTAGTGGGTCATCTGATTCTGTGGCCACACTGAACAGTGAAG
  AATTTGTTTTGGTTTCTCAGCACACAGATGCCACTTCTATAAAGGATGATGGGAAGCCACAGCTGAAGAT
  AGCTTCCAATGGTGATGAGCAGTTGGAAAAAGCCATGGAAGAGATTTTGAGAGATTCCGAGAAAGGACAA
  AGCGGTCTACCTGTTGATTGCCAAGGATCCAGTGAGATTTCAGACTGTCCCTTTGGAGATGTGCCGGCCA
  GCCAAACAACTAAGCCGCCTCTCCAGTTAATTTTGGATCCATCTAATACAGAAATTTCCACACCCAGACC
  ATCTTCTCCAAGCAGATTTCCTGAAGAAGACAGTGTTCTCTTTAACAAGCTGACATACTTAGGATGTATG
  AAGGTTTCTTCCCCACGCAGTGAAGTGGAGGCTTTACGGGCCATGGCCACCATGAGAGCTTCCAGTCAGT
  ACCCCTTTGCTGTTACTCTGTATGTGCCCAATGTTCCAGAAGGATCTGTGAGAATCATAGACCAGTCAAG
  CAATGTGGAGATAGCATCTTTTCCAATTTATAAAGTGCTTTTCTGTGCACGTGGGCATGATGGGACAGCC
  GAGAGCAATTGCTTTGCATTTACAGAGAGTTCTCATGGCTCAGAAGAATTTCAGATACATGTTTTCTCCT
  GTGAAATTAAAGAGGCAGTAAGCAGAATTTTATATAGTTTCTGCACTGCATTCAAACGTTCTTCCAGACA
  AGTGTCTGATGTTAAAGACTCAGTCATCCCGACTCCCGACAGTGATGTGTTTACCTTCAGTGTTTCCTTG
  GAGGTCAAAGAAGATGATGGAAAAGGAAACTTTAGTCCCGTGCCTAAGGATAGAGATAAATTTTATTTCA
  AAATAAAGCAAGGAATAGAGAAGAAGGTTGTGATTACAGTTCAGCAACTGTCTAACAAAGAATTAGCTAT
  TGAGAGATGTTTTGGAATGTTATTAAGCCCAGGTCGAAACGTGAAGAACAGTGACATGCATTTACTGGAT
  ATGGAGTCCATGGGAAAGAGCTATGATGGGAGAGCTTACGTCATCACGGGCATGTGGAACCCCAACGCAC
  CAATATTTCTGGCTCTTAATGAAGAAACGCCCAAAGATAAACGGGTGTACATGACCGTTGCAGTGGACAT
  GGTGGTCACAGAGGTGGTGGAGCCAGTCCGTTTTCTCCTGGAGACAGTAGTTCGTGTATACCCTGCAAAT
  GAGAGATTTTGGTATTTCAGCAGAAAGACTTTCACAGAGACTTTCTTCATGAGGTTGAAACAGTCTGAGG
  GAAAAGGCCACAGCAGTGCTGGAGATGCAATATATGAAGTGGTGAGTCTGCAGAGAGAATCTGACAAGGA
  GGAGCCCATCACCCCTACTAGTGCTGGGGGCCCAATGTCACCACAGGAGGATGAAGCAGAAGAGGAGAGT
  GATAATGAACTCTCGAGTGGAACAGGTGATGTGTCAAAAGACTGTCCTGAGAAGATTCTCTATTCTTGGG
  GAGAATTGTTAGGAAGATGGCACAATAACCTTGGTGGCCGACCAAAAGGACTATTCACCTTGGTAAAGAG
  TGGTGTTCCTGAGGCACTAAGGGCAGAAGTATGGCAACTATTAGCAGGATGCCATGACAATCAGGAAATG
  CTGGATAAATACCGAATCCTTATCACAAAGGACTCTGCACAGGAGAGTGTTATTACTCGAGATATTCATC
  GTACATTTCCTGCACACGATTACTTCAAAGACACTGGAGGCGATGGTCAAGAATCCCTTTACAAAATCTG
  CAAGGCCTACTCGGTGTTCGATGAAGACATTGGGTACTGTCAAGGACAGTCTTTTCTTGCTGCTGTACTA
  CTGTTGCATATGCCAGAGGAGCAAGCATTCTGTGTTTTGGTGACTATTATGTATGGTTATAAATTGAGAG
  ACTTATACAGAAACAATTTTGAAGATCTTCATTGCAAATTCTATCAACTTGAGAAACTAATGCAGGAACA
  GCTGCCAGACCTGTACAGCCACTTCTGTGATCTGAACCTGGAAGCTCATATGTATGCATCCCAGTGGTTC
  CTCACTCTCTTTACTGCCAAGTTTCCACTCTGCATGGTGTTCCACATCATTGACCTTCTGCTGTGTGAGG
  GTTTGAATATAATCTTTCATGTAGCTTTGGCTCTTCTAAAGACCTCAAAGGAAGACCTCCTTCAGGCTGA
  TTTTGAAGGTGCTTTGAAGTTTTTCAGAGTTCAGCTTCCAAAGAGATACCGGGCAGAGGAGAATGCAAGA
  AGATTGATGGAACAGGCTTGCAATATTAAAGTACCAACCAAAAAGCTGAAGAAATATGAAAAAGAATATC
  AGGCGATGCGGGAGAATCAGCTGCAGCAGGAGGACCCAATGGATAGATACAAGTTTGTATATTTATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_013862
• Insert Size: 2448 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013862.5, NP_038890.3
• RefSeq Size: 3373 bp
• RefSeq ORF: 2448 bp
• Locus ID: 29809
• UniProt ID: A6H6A9
• Cytogenetics: 1 H2.1
• Gene Summary: GTP-hydrolysis activating protein (GAP) for small GTPase RAB22A, converting active RAB22A-GTP to the inactive form RAB22A-GDP (By similarity). Plays a role in endocytosis and intracellular protein transport. Recruited by ANK2 to phosphatidylinositol 3-phosphate (PI3P)-positive early endosomes, where it inactivates RAB22A, and promotes polarized trafficking to the leading edge of the migrating cells. Part of the ANK2/RABGAP1L complex which is required for the polarized recycling of fibronectin receptor ITGA5 ITGB1 to the plasma membrane that enables continuous directional cell migration (PubMed:27718357).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.