Lrrc8a (NM_177725) Mouse Untagged Clone

CAT#: MC221780

Lrrc8a (untagged) - Mouse leucine rich repeat containing 8A (Lrrc8a), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Lrrc8a
• Synonyms: Lrrc8; mKIAA1437
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221780 representing NM_177725
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTCCGGTGACAGAGCTCCGCTACTTTGCGGACACACAGCCAGCATACCGGATCCTGAAGCCTTGGT
  GGGATGTGTTCACTGATTACATCTCCATCGTCATGCTGATGATTGCTGTCTTTGGAGGGACGCTGCAAGT
  CACCCAGGACAAGATGATCTGCCTACCTTGTAAGTGGGTCACCAAAGACTCCTGCAACGACTCCTTCCGG
  GGCTGGGCAGCCTCCAGCCCAGAGCCCACTTACCCCAACTCCACAGTCCTGCCGACGCCTGACACAGGCC
  CCACAGGTATCAAGTATGACCTAGATCGACACCAGTACAACTACGTGGATGCGGTGTGCTACGAGAACCG
  CCTGCATTGGTTTGCCAAGTACTTCCCCTACCTCGTGCTTCTGCACACCCTCATCTTCCTGGCCTGTAGC
  AACTTCTGGTTCAAGTTTCCACGCACCAGTTCGAAGCTGGAACACTTTGTGTCTATCCTGCTCAAGTGCT
  TCGACTCACCGTGGACCACACGGGCCCTGTCGGAGACAGTGGTGGAGGAGAGTGACCCCAAGCCAGCCTT
  CAGCAAGATGAACGGGTCCATGGACAAGAAGTCATCTACAGTCAGCGAGGATGTGGAGGCCACCGTGCCC
  ATGCTGCAGCGGACCAAGTCACGGATTGAGCAGGGCATCGTGGACCGATCGGAGACGGGCGTACTGGACA
  AGAAGGAAGGGGAGCAGGCGAAGGCCCTGTTTGAGAAGGTGAAGAAGTTCCGGACTCACGTGGAGGAGGG
  GGACATTGTGTACCGCCTGTACATGCGGCAGACCATCATCAAGGTGATCAAGTTCGCCCTCATCATCTGC
  TACACCGTCTACTATGTGCACAACATCAAGTTCGACGTGGACTGCACCGTGGACATCGAGAGCCTGACAG
  GCTACCGCACCTACCGCTGTGCCCACCCCCTGGCCACGCTCTTCAAGATCTTGGCATCCTTCTACATCAG
  CTTGGTCATCTTCTATGGCCTCATCTGCATGTACACACTGTGGTGGATGCTGCGGCGCTCCCTCAAGAAG
  TACTCGTTCGAGTCGATCCGAGAGGAGAGCAGCTACAGTGACATCCCCGACGTCAAGAACGACTTTGCCT
  TCATGTTGCACCTCATCGACCAGTATGACCCTCTCTACTCAAAGCGCTTCGCCGTCTTCCTGTCTGAGGT
  GAGTGAAAACAAGCTGCGCCAGCTGAACCTCAACAACGAGTGGACGCTGGACAAGCTGCGCCAGCGCCTC
  ACCAAGAACGCCCAGGACAAGCTGGAGCTGCACCTGTTCATGCTCAGCGGCATCCCGGACACTGTGTTTG
  ACCTGGTTGAGCTCGAGGTCCTGAAGCTGGAGCTGATACCCGACGTGACCATCCCGCCCAGCATCGCCCA
  GCTCACAGGCCTCAAGGAGCTGTGGCTGTACCACACGGCAGCCAAGATCGAGGCTCCTGCCCTGGCCTTC
  CTGCGGGAGAACCTGCGGGCCCTGCACATCAAGTTCACGGACATCAAGGAGATCCCGCTGTGGATCTACA
  GCCTGAAGACGCTGGAGGAGCTGCACCTGACGGGCAACCTGAGTGCAGAGAACAACCGCTACATCGTCAT
  TGACGGGCTGCGGGAGCTCAAACGCCTCAAGGTGCTGCGACTCAAGAGCAACCTGAGCAAGCTGCCGCAG
  GTGGTCACGGATGTGGGCGTGCACCTGCAGAAGCTGTCCATCAACAATGAGGGCACCAAGCTCATTGTCC
  TCAACAGCCTCAAGAAGATGGTTAACCTGACGGAGCTAGAGCTGATCCGCTGTGACCTGGAGCGCATCCC
  TCACTCCATCTTTAGCCTCCACAACCTGCAGGAGATTGACCTGAAGGACAACAACCTCAAGACCATTGAG
  GAGATCATCAGCTTCCAGCACCTGCACCGCCTCACCTGCCTTAAGCTGTGGTACAACCACATTGCCTACA
  TCCCCATCCAGATCGGCAACCTCACCAACCTCGAGCGCCTCTACCTGAACCGCAACAAAATCGAGAAAAT
  CCCCACCCAGCTCTTCTACTGCCGCAAGCTGCGCTACCTGGACCTCAGCCACAACAACCTGACCTTCCTC
  CCCGCCGACATTGGCCTCCTGCAGAACCTCCAGAACCTGGCCGTCACAGCCAATAGGATTGAAGCGCTCC
  CCCCAGAGCTCTTCCAGTGTCGGAAGCTACGGGCTCTACACCTGGGCAACAATGTACTGCAGTCGCTGCC
  CTCGAGAGTGGGCGAGCTGACCAACCTGACGCAGATCGAGCTTCGGGGCAACCGGCTGGAATGCCTGCCC
  GTGGAGCTGGGAGAGTGCCCCCTACTCAAGCGCAGCGGCCTGGTGGTGGAAGAGGACTTGTTCAGCACAC
  TGCCACCTGAGGTGAAGGAGCGGCTCTGGAGAGCTGACAAGGAGCAGGCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_177725
• Insert Size: 2433 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177725.4, NP_808393.1
• RefSeq Size: 4254 bp
• RefSeq ORF: 2433 bp
• Locus ID: 241296
• UniProt ID: Q80WG5
• Cytogenetics: 2 B
• Gene Summary: Essential component of the volume-regulated anion channel (VRAC, also named VSOAC channel), an anion channel required to maintain a constant cell volume in response to extracellular or intracellular osmotic changes (PubMed:29769723). The VRAC channel conducts iodide better than chloride and can also conduct organic osmolytes like taurine (By similarity). Mediates efflux of amino acids, such as aspartate and glutamate, in response to osmotic stress (By similarity). Required for channel activity, together with at least one other family member (LRRC8B, LRRC8C, LRRC8D or LRRC8E); channel characteristics depend on the precise subunit composition. Can form functional channels by itself (in vitro) (By similarity). Involved in B-cell development: required for the pro-B cell to pre-B cell transition (PubMed:14660746, PubMed:24752297). Also required for T-cell development (PubMed:24752297).[UniProtKB/Swiss-Prot Function]