Pik3ap1 (NM_031376) Mouse Untagged Clone

CAT#: MC221771

Pik3ap1 (untagged) - Mouse phosphoinositide-3-kinase adaptor protein 1 (Pik3ap1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pik3ap1
• Synonyms: 1810044J04Rik; BCAP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221771 representing NM_031376
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCCTCAGGGTGGGGCCGGGGATGCGACATCCTCATCTTCTACAGCCCAGATGCGGAGGAATGGT
  GTCAGTACCTACAGGATCTTTTCGTGTCCTGCCGGCAGGTTCGTAGCCAGAAGACGCAGACATACCGGCT
  GGTCCCGGATGCCTCTTTCTCTGCCCAGGACCTGTGGGTCTTCAGGGATGCGCGATGCGTGCTGGTGCTA
  CTGTCGGCGGGGCTGGTAGGATGCTTCGGCCAGCCAGGACTGCTGCCAATGCTGCAGCGTGCCTGCCATC
  CGCCGCAGCGTGTGGTGCGGCTGTTGTGTGGAGTGCAGCCAGGCGACGAGGACTTCCAGGCCTTCTTTCC
  TGACTGGGCCCACTGGCAGGAAATGACTTGTGATGATGAGCCAGAGACCTACCTGGCGGCTGTGAGGAAG
  GCCATTTCTGAAGATTCTGGCTGTGACTCAGTAACCGACACGGAGCCCGAGGACGAGAGAGAGCTTCCTT
  TCTCAAAGCAGACAAACCTGCCTCCGGAGATATCGCCTGGGAACCTGATGGTGGTACAGCCGGACCGCAT
  TCGCTGTGGGGCAGAAACCACTGTGTATATCATTGTGAGATGCAAGCTGGACGAGAAGGTATCAACAGAA
  GCAGAGTTCTCTCCTGAGGATTCTCCCTCCATCCGGGTGGAAGGCACACTGGAGAATGAGTACACGGTCT
  CTGTGAAAGCACCAGACCTCTCCTCGGGGAATGTTTCTCTGAAGGTCTATTCTGGGGACCTGGTGGTCTG
  CGAAACCACCGTCAGCTATTATACCGACATGGAGGAAATTGGGAACTTACTGTCGAGTGCTGCAAATCCT
  GTGGAGTTTATGTGTCAGGCCTTTAAAATCGTGCCCTACAACACAGAGACTCTCGATAAACTGCTCACCG
  AGTCCCTGAAGAACAACATCCCCGCAAGTGGACTGCACCTCTTTGGGATCAACCAACTGGAAGAAGACGA
  TATGATGACAAATCAGAGGGACGAAGAGCTGCCCACGCTGTTGCATTTTGCTGCCAAGTATGGACTGAAG
  AACCTCACTGCCTTGCTGCTCACCTGTCCAGGGGCTCTCCAGGCCTACAGTGTAGCCAACAAGCACGGCC
  ACTATCCCAACACCATTGCCGAGAAACACGGCTTCAGGGACCTGCGCCAGTTCATCGACGAGTATGTGGA
  GACGGTGGACATGCTGAAGACACACATTAAGGAGGAGTTGATGCAAGGGGAGGAGGCGGACGATGTGTAC
  GAGTCCATGGCCCACCTATCCACAGACCTGCTGATGAAGTGCTCCCTCAACCCTGGCTGTGACGATGAGC
  TCTACGAGTCCATGGCTGCCTTCGCCCCAGCAGCCACAGAAGACCTCTATGTTGAAATGCTTCAGGCCAG
  TGCAGGTAACCCAGTCTCGGGAGAAAGTTTCTCTCGGCCCACTAAAGACTCGATGATCCGCAAGTTTCTA
  GAAGGTAACAGTGTGAAACCGGCCAGTTGGGAGAGAGAACAACACCATCCCTATGGGGAGGAACTTTATC
  ACATTGTGGATGAAGATGAGACCTTCTCTGTGGACCTAGCCAACAGGCCCCCTGTCCCTGTGCCCAGGCC
  AGAGGCCAGCGCTCCTGGCCCTCCCCCACCGCCTGACAATGAGCCATACATTTCTAAAGTTTTTGCAGAA
  AAAAGTCAGGAGCGGCTCGGGAATTTCTACGTTTCTTCTGAGAGCATCAGAAAAGAGCCACTTGTGAGGC
  CATGGAGGGACAGGCCGCCATCGAGCATCTACGACCCATTTGCTGGAATGAAGACCCCGGGCCAGCGGCA
  GCTCATCACCCTGCAGGAGCAGGTGAAGCTGGGCATCGTCAACGTGGACGAGGCCGTGCTGCATTTCAAA
  GAGTGGCAACTCAATCAGAAGAAGCGATCCGAGTCCTTCCGCTTCCAGCAGGAAAATCTTAAAAGACTAA
  GAGAGAGCATCACCCGAAGACGGAAAGAGAAGCCAAAGTCTGGGAAGCACACAGACCTGGAGATCACAGT
  CCCAATCCGACATTCACAGCACCTGCCAGAGAAAGTGGAGTTTGGCGTGTATGAGAGCGGCCCCAGGAAG
  AGTGTCTTGCCGGCCAGGACAGAGCTGAGACGTGGGGACTGGAAAACGGACAGCATGTCCAGCACAGCAA
  GCAGCACGAGTAACCGGTCGAGCACACGGAGCCTCCTCAGTGTGAGCAGTGGAATGGAGGGCGACAACGA
  GGACAACGAAATCCCCGAAATTACCCGGAGTCGTGGCCCAGGTCCTACGCAGGTGGACGGAGCTCCAGTT
  GTGACCGGAACACCAGTCGGGACCCTTGAGAGACCCCCTAGGGTGCCTCCTCGAGCCGCTTCTCAGAGGC
  CTCTAACCCGGGAGTCCTTCCATCCTCCTCCGCCGGTTCCACCCAGAGGTCGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_031376
• Insert Size: 2436 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031376.3, NP_113553.1
• RefSeq Size: 2615 bp
• RefSeq ORF: 2436 bp
• Locus ID: 83490
• UniProt ID: Q9EQ32
• Cytogenetics: 19 C3
• Gene Summary: Signaling adapter that contributes to B-cell development by linking B-cell receptor (BCR) signaling to the phosphoinositide 3-kinase (PI3K)-Akt signaling pathway. Has a complementary role to the BCR coreceptor CD19, coupling BCR and PI3K activation by providing a docking site for the PI3K subunit PIK3R1. Alternatively, links Toll-like receptor (TLR) signaling to PI3K activation, a process preventing excessive inflammatory cytokine production. Also involved in the activation of PI3K in natural killer cells. May be involved in the survival of mature B-cells via activation of REL.[UniProtKB/Swiss-Prot Function]