Perm1 (NM_172417) Mouse Untagged Clone

CAT#: MC221766

2310042D19Rik (untagged) - Mouse RIKEN cDNA 2310042D19 gene (2310042D19Rik), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Perm1
• Synonyms: 2310042D19Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221766 representing NM_172417
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACAACTTCCAGTACAGCGTCCAGCTCAGCGATCGGGAGTGGGCCGAGTTTTCAGCCACTGCTGATG
  AGTGTGGCCTCTTGCAGGCTGACCTGGCCTCTGGTGACGAGCCCTTGTCCAGTGACATTGACCAAGGGGA
  CAGCAGTGGAAGCAGCCCCCCTGGGCCCCCACCCCTCTTCACTGGGCAGCTGGTTTCCCAAGGGAGGGGA
  CAACAGAGCCGTGAGTTGGAGGACGTGGCTGCTCAGCAGCTGGTCAGCAGGTCTCAGTGTGAGCCTGTCC
  TGGCTCTGGAGGCCAGTCATCAGGTAGCCGGCACGTCCACACAGTCAGAGGCCCCTCTGTTCCCCAGCTT
  AGACAGTGTCTGTCCTGGCCAGGCCTTGTCCTTTCCAGGGCCGGCAACTTGCAGAGACAAGATGCAGAGG
  CTTTTGCAAGGCCCTGCCCCTAGCTCTCCCAGTAAAGCCCCTCATAGTCCTGAGTCCCCTGGCCATAGCG
  ACAACCCTCAGAGTTCCCCTGACAGCCTCGAAGCTTCACCGCGGAACCCTGGTCGTAAGAAGAGGCGTGC
  TGTAGGTGCCAAGGGGACCAAGCACTCCGGATCTCTGGACTCTGCTGCTACCCAGATGAGCTCCCCACAG
  CTTACCAGGCCCAAGGAGGCTCTCATGTCTGGGACACTGATGGCAAAAGCCCAGCAGGATAAGCCACAGC
  CTGACTCCACGAGTCCTGAACAGATGGCCAGAGAGGAGTCAGGCTTGGATCTTTCTACACCTATTCTGAT
  AACTGAACAAGATCAGATCAGAAAGACATCCAGAGCTGCGTTACATGTGGTATCCAAACCTGTCCAGGAA
  GTTCACCCAGATGTCCCCATGGCCTCACCAAATGTGTCTACACGTGCCTCCAAGCCTCAACCCGACGTGG
  CTTTGCCTAAACCTGCCTCCAAGCCGCAGTCTGACATAGCTTCGTCTACACATCCCTTCACGCCTGACGT
  GTCTTTGTCCACACTTGCCTTCAAATGTCAACCCAATACAAATCAGTCTACACCCGCCTCTGAGCCTGAC
  ATGGCTTTGTCTACACCTGCCTCTGAGCCTGACACGGCTTTGTCTACACCCGCCTCTGAGCCTGACACGG
  CTTTGTCTACACCCGCCTCTGAGCCTGACACGGCTTTGTCTACACCCGCCTCTAGGTCGCAACTGGTGAA
  GGCTGAATTTGCGTCTGCTTGCACACCTGGATTGCATGTGGACCTCTCTGCAGCAGGCTCAGAGATCAAG
  CCAGAAGTTTCTTCCTCTATGCCTGCCGCAGTGGATGTGCTTCGTACAGATCTGCCTCGTTCTGTTTCAA
  AGACTGAGTCTAAAGAGAGTGTGTCTATACCTGTTAAGCCTTCTTTATCCCCTATTTCCCAGGCTGAAGC
  TGCCATGGTGGATACAGGTGTGTCTGTACCTCCAGGGGGAAGCATTGAGAAGCCAGCAGGGCAGTTCTCA
  GCAGGGTCCTCAGGAGAGTCCTGCCTAGGTCCCGTCCAAGCCCCCAAGAAGAAGAAAGTACGATTTTCCA
  TGGCTATCCCCAGCCATGAAGACTCAGGGTCAGGGGAGCCTACAGGTTCACCCTTCCTAACCCCGGAGCA
  GCCCCCAGTTCCCAGGACAGCAGCAGGAAGCCGTGGGGGGTCTGCAGCCTGGGATGCTGTGGCTGTCGCG
  CCCCGACTCCCCCAGCCTCGGATCCTCAAGCACCTGCCTCCTCCTGTATCCTCTGTATCAGCAGAGGCTG
  AGTCTGGAAACTGCTTTGCAGTGACTCTCCCTGAGGCCTACGAGTTCTTCTTCTGTGACACCATTGAGGA
  GGAAGATGAAGACGTAGAGGACGAAGAGGCAGCCAGCCAAGCCCTGGATGAAGTCCAATGGCCTGACACA
  TGCGAGTTTTTTTTTCGGGATAGCAGAGCCCAAAGATCAAGATGTCAGAGGGGACACTCTCCAGTCTCAC
  CACCAAGAGCAGACACCGTGGCACCTGTCCCCCCTGAAGGCTTGGTGCCTATTTCCATCCCTGAGGTCTA
  TGAACACTTCTTCACTGAGGAAGGATTTGGGCACAGACAGCCTGCGGCCACTCCTATGCAGACCTCAGAG
  CTTTCCAGGGAAGGAGGGCTTGAGGCATCCACCAAGCCTGTTTCACCTACAGCGGAACATCTTAGCTTAA
  CAGTCAGGAAGGCAGGGGAACTACGGAGCCCCCTCACTTCATTTACGTTCAGTCAAAACGACATGTGCCT
  GGTGTTTGTTGCCTTTGCCACTTGGGCTGTGAGAACCTCAGATCTACAAGCCCCAGATGCTTGGAAAACA
  GTCTTGCTCGCCAACATTGGCACCATCTCTGCTATCCGCTACTTCCGCCGTCAGGTTGGGAGAGGCCATA
  GTGGCAGACCGAGATCCAGTTCCAGCTCAAACCCCAGCTGCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_172417
• Insert Size: 2424 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172417.3, NP_766005.2
• RefSeq Size: 3849 bp
• RefSeq ORF: 2424 bp
• Locus ID: 74183
• UniProt ID: Q149B8
• Cytogenetics: 4 E2
• Gene Summary: Regulates the expression of selective PPARGC1A/B and ESRRA/B/G target genes with roles in glucose and lipid metabolism, energy transfer, contractile function, muscle mitochondrial biogenesis and oxidative capacity. Required for the efficient induction of MT-CO2, MT-CO3, COX4I1, TFB1M, TFB2M, POLRMT and SIRT3 by PPARGC1A. Positively regulates the PPARGC1A/ESRRG-induced expression of CKMT2, TNNI3 and SLC2A4 and negatively regulates the PPARGC1A/ESRRG-induced expression of PDK4.[UniProtKB/Swiss-Prot Function]