Taf5 (NM_177342) Mouse Untagged Clone

CAT#: MC221733

Taf5 (untagged) - Mouse TAF5 RNA polymerase II, TATA box binding protein (TBP)-associated factor (Taf5), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Taf5
• Synonyms: 6330528C20Rik; AV117817
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221733 representing NM_177342
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGCTGGCGGAGGAGCAGACGGAGGTGGCGGTCAAGCTAGAGCCTGAGGGACCGCCGACGCTGC
  TACCTCCGCAGGCTGGCGACGGCGCAGGCGAGGGGAGCGGCGGCACTCCCAACAACGGCCCCAACGGCGG
  CGGCGGCGGGAATGTGGCAGTGGCAGCGGCGGCCGGCGGGGACGGCGGGACCCCCAAGCCTGGGGTGGCT
  GTCTCCGCCGCTGCCCCGGCGGGGGCGGCCCCGGTGCCCGCCGCTCCGGCGGAGGCCGGCGCTCCCCACG
  ACCGACAGACCCTCCTGGCCGTGCTGCAATTCCTCCGGCAGAGCAACCTCCGCGAGGCCGAAGAGGCGCT
  GCGCCGGGAGGCCCGGCTGCTGGAGGAGGCGGTGGCGGGCTCCGGAGCTCCGGGAGAGTTGGACGGGGCC
  GGCGCGGAGGCGGCCAGCGCGCTTCTCAGCCGGGTCACCGCTTCAGTTCCCGGCTCTGCGGCCCCCGAGC
  CTCCGGGGACGGGCGCCTCGGTGACCTCGGTGTTCTCGGGTTCAGCCTCAGGTCCTGCGGCTCCGGGAAA
  GGTTGCAAGTGTAGCTGTGGAAGACCAGCCAGACGTCAGTGCTGTCTTGTCGGCCTACAACCAACAAGGA
  GACCCTACAATGTATGAAGAATACTACAGTGGACTGAAACACTTCATTGAATGTTCTCTGGACTGCCATC
  GAGCAGAGTTATCCCAACTCTTTTATCCTCTGTTTGTACATATGTACTTGGAGCTAGTTTATAATCAACA
  TGAAAATGAAGCAAAATCATTCTTTGAGAAGTTCCATGGAGATCAGGAATGTTACTACCAGGATGACCTA
  CGAGTGTTATCTAGTCTTACCAAAAAGGAGCACATGAAAGGGAACGAGACCATGCTGGACTTTCGGACGA
  GTAAATTCGTTCTCCGCATTTCCCGGGACTCGTACCAGCTCTTGAAGCGGCATCTCCAGGAGAAGCAGAA
  CAATCAGATCTGGAACATCGTGCAGGAGCACCTCTACATCGACATCTTCGACGGGATGCCTCGCAGTAAG
  CAACAGATCGACGCCATGGTGGGCAGCCTGGCAGGAGAGGCGAAACGAGAGGCGAACAAATCGAAGGTAT
  TTTTTGGTTTACTAAAAGAACCAGAAATTGAGGTGCCTTTGGATGATGAGGACGAAGAAGGAGAAAATGA
  AGAAGGAAAACCTAAAAAGAAGAAGCCTAAGAAGGATAGTATTGGCTCTAAAAGCAAAAAACAAGATCCC
  AATGCCCCGCCTCAAAACAGAATTCCTCTTCCTGAGTTAAAAGATTCAGACAAACTGGACAAAATAATGA
  ATATGAAAGAAACTACCAAACGAGTGCGCCTCGGGCCAGACTGTTTACCCTCCATTTGTTTCTATACATT
  CCTTAATGCTTACCAGGGCCTCACTGCAGTGGATGTCACTGATGACTCTAGTCTGATTGCTGGAGGTTTT
  GCTGATTCAACTGTCAGAGTGTGGTCTGTGACCCCTAAAAAGCTTCGTAGTGTCAAACAAGCATCAGATC
  TTAGTCTTATAGACAAAGAATCAGATGATGTCTTAGAGAGAATCATGGATGAGAAAACTGCAAGTGAGTT
  GAAGATTTTGTATGGTCACAGTGGGCCTGTCTATGGAGCCAGCTTCAGTCCGGATAGGAACTACCTACTC
  TCCTCTTCAGAGGATGGAACTGTTAGACTCTGGAGCCTTCAGACTTTCACTTGCTTGGTTGGGTATAAAG
  GACACAACTACCCAGTATGGGACACACAGTTTTCTCCATACGGATATTATTTCGTGTCAGGAGGCCATGA
  CCGAGTAGCTCGGCTTTGGGCTACCGATCACTATCAGCCTTTGAGGATATTTGCTGGCCATCTTGCTGAT
  GTGAATTGCACTAGATTTCATCCAAATTCGAACTATGTTGCTACGGGCTCTGCAGACAGAACTGTGCGGC
  TCTGGGATGTCCTCAATGGTAACTGTGTAAGAATCTTCACTGGGCACAAGGGACCAATTCATTCCTTGAC
  ATTTTCTCCCAATGGGAGATTCCTGGCTACAGGAGCAACAGATGGCAGAGTACTTCTTTGGGATATTGGA
  CACGGCTTGATGGTTGGAGAATTAAAAGGCCACACTGATACAGTGTGTTCACTTAGGTTTAGTAGAGATG
  GTGAAATTTTGGCATCAGGTTCAATGGATAATACAGTCCGGTTATGGGACGCCGTCAAAGCGTTTGAAGA
  CTTAGAGACTGATGACTTTACTACAGCCACCGGGCATATAAACCTACCTGAGAATTCCCAGGAGTTGTTA
  TTGGGTACATATATGACCAAATCAACACCAGTTGTACACCTACATTTTACAAGAAGAAACTTGGTTCTAG
  CTGCAGGAGCTTACAGTCCGCAATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_177342
• Insert Size: 2406 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177342.3, NP_796316.2
• RefSeq Size: 3258 bp
• RefSeq ORF: 2406 bp
• Locus ID: 226182
• UniProt ID: Q8C092
• Cytogenetics: 19 C3
• Gene Summary: TAFs are components of the transcription factor IID (TFIID) complex, PCAF histone acetylase complex and TBP-free TAFII complex (TFTC). TAFs components-TIIFD are essential for mediating regulation of RNA polymerase transcription. TAF5/TAFII100 interacts strongly with the histone H4-related TAF6/TAFII80 and the histone H3-related TAF9/TAFII31, as well as a stable complex comprised of both TAF5/TAFII80 and TAF6/TAFII31. Apparently weaker interactions of TAF5/TAFII100 with TBP, TAF1/TAFII250, TAF11/TAFII28, and TAF12/TAFII20, but not TAF7/TAFII55, also have been observed (By similarity).[UniProtKB/Swiss-Prot Function]