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Gpat2 (NM_001081089) Mouse Untagged Clone

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SKU
MC221731
Gpat2 (untagged) - Mouse glycerol-3-phosphate acyltransferase 2, mitochondrial (Gpat2), nuclear gene encoding mitochondrial protein, (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $808.00
5 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Gpat2
Synonyms A530057A03Rik; Gm116; xGPAT1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC221731 representing NM_001081089
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATACCATGTTGAAATCCAACCCCCAAACCCAGCAGAGGAGTAACCACAATGGTCAAGAGACCAGCC
TTTGGTCCTCAAGCTTTGGGATGAAGATGGAGGCCATCACTCCATTCCTGGGAAAGTATCGCCCCTTTAT
GGGCCGCTGCTGCCAGACCTGTACTCCTAAGAGCTGGGAGTCCCTCTTCCACAGAAGCATAATGGACCTG
GGCTTCTGCAACGTGATCCTTGTGAAGGAGGAGAACACCAGGTTCCGGGGCTGGCTGGTTCGCAGGCTCT
GCTATTTCCTGTGGTCCCTGGAGCAGCACATACCCACCAGTTTTGATGCCTCCCAGAAGATCATGGAAAA
CACTGGGGTGCAGAACCTCCTCTCGGGGAGGGTTCCGGGAGCGGCTGGAGAAGGCCAGGCGCCTGAATTA
GTGAAGAAAGAGGTACAGCGTATCCTGGGCCACATTCAGACCACACCCCGCCCCTTCTTGCTCAGGCTGT
TCAGCTGGGCACTGCTGTGGTTCCTGAACCGCCTCTTCCTGAATGTACAACTTCACAAGGGACAGATGAA
GATGGTTCAGAAGGCTGTGCAGGAGGGCTCTCCACTTGTCTTCCTTTCTACACACAAGTCCCTCTTGGAT
GGATTCCTGCTGCCTTTTGTACTGTTCTCCCAAGGCCTCGGTGTGGTCCGTGTGGCTTTAGACTCCCGCA
CATGTTCCCCTGCTCTCAGAGCTCTATTGAGGAAACTTGGGGGACTTTTCCTGCCCCCAGAGGTCAACCT
CTCCTTGGACAACTCAGAGGGCATCCTTGCAAGGGCTGTGGTCCGTGCAACTGTGGAGGAGCTGCTTACT
AGTGGTCAGCCTCTGCTCATCTTCCTGGAAGAGCCCCCTGGGTCCCCAGGGCCCAGGCTTTCTGCCCTGG
GCCAGGCCTGGCTAGGAGTGGTAATCCAGGCGGTCCAGGCAGGCATTATCTCAGATGCCACACTGGTACC
AGTTGCCATTGCCTATGACCTGGTTCCAGATGCACCATGTAACATGAACCATGATTTGGCTCCCCTGGGG
CTATGGACAGGAGCCTTGGCTGTCTTTCGGAGACTGTGTAACTGCTGGGGCTGCAACCGTAGAGTCTGTG
TTCGGGTGCACCTTGCCCAGCCATTTTCCCTCCAGGAATACACCATCAATGCTAGAAGTTGCTGGGATAG
CAGGCAGACCCTGGAACACTTGTTGCAGCCCATTGTGCTAGGTGAATGTAGTGTTGTTCCGGACACTGAG
AAGGAGCAGGAGTGGACCCCACCAACTGGCCTCCTCCTGGCCCTTAAAGAGGAGGACCAGCTCCTGGTCA
GAAGACTGAGCCGGCATGTTCTGAGTGCCAGTGTGGCCAGCTCCGCAGTGATGAGCACAGCCATCATGGC
CACACTGCTTTTGCTGAAGCACCAAAAGGGTGTGGTCCTGTCACAGCTCCTGGGAGAATTCTCCTGGCTG
ACAGAGGAGACACTGCTCCGAGGTTTTGATGTGGGCTTCTCAGGGCAGCTGCGGTGCCTGGCCCAACATA
CACTGAGCCTATTGAGGGCACATGTGGTTCTGCTGCGTGTCCACCAAGGGGACTTGGTGGTGGTACCTCG
GCCTGGCCCAGGCCTCACACACCTGGCACGCTTGAGTATGGAATTGCTGCCAACCTTCCTGAGTGAGGCT
GTGGGTGCCTGTGCAGTGCGGGGGTTGCTGGCAGGCAGAGTACCACCTGAGGGGCCCTGGGAGCTGCAGG
GCATAGAGCTACTGAGTCAGAATGAACTGTACCGCCAGATCCTACTGCTGCTGCACCTGCTACCTCAAGA
CCTACTGCTGCCCCAGCCCTGCCAGTCTTCCTACTGCTACTGTCAGGAAGTGCTAGACCGGCTCATCCAG
TGTGGACTCCTAGTTGCTGAGGAGACTCCAGGCTCCCGGCCAGCCTGTGACACAGGAAGACAGCATCTGA
GTGCAAAGCTGCTGTGGAAGCCGAGTGGGGACTTTACTGACAGTGAGAGTGATGACTTTGAGGAGCCAGG
AGGCCGCTGCTTCAGGCTTAGCCAGCAATCCCGCTGCCCTGACTTCTTCCTCTTCCTCTGCCGCTTGCTC
AGTCCCATACTCAAGGCCTTCGCACAGGCGGCCACCTTCCTCCACTTAGGGCAGCTGCCAGATTCAGAGG
TGGCCTACTCAGAGAAGTTGTTCCAGTTTTTACAGGCCTGTGCCCAAGAAGAAGGGATCTTTGAGTGTGC
AGACCCAAACCTTGCTATCAGTGCAGTCTGGACGTTCAAAGATCTGGGGGTACTTCAGGAGATGCCTAGC
CCTACAGGACCCCAGCTCCACCTGTCCCCTACATTTGCCACCCGGGACAATCAGGACAAGCTGGAACAAT
TTATCCGACAGTTCATCTGCAGTTAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_001081089
Insert Size 2406 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001081089.2, NP_001074558.2
RefSeq Size 2636 bp
RefSeq ORF 2406 bp
Locus ID 215456
UniProt ID Q14DK4
Cytogenetics 2 F1
Summary Esterifies acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate, an essential step in glycerolipid biosynthesis (PubMed:17013544, PubMed:17689486). Required for primary processing step during piRNA biosynthesis (PubMed:23611983). Molecular mechanisms by which it promotes piRNA biosynthesis are unclear and do not involve its acyltransferase activity (PubMed:23611983).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001081089" in other vectors (4)
SKU Description Size Price
MG218359 Gpat2 (tGFP-tagged) - Mouse glycerol-3-phosphate acyltransferase 2 mitochondrial (Gpat2) nuclear gene encoding mitochondrial protein, (10ug) 10 ug
$932.00
MR218359 Gpat2 (Myc-DDK-tagged) - Mouse glycerol-3-phosphate acyltransferase 2, mitochondrial (Gpat2), nuclear gene encoding mitochondrial protein 10 ug
$589.00 $732.00
MR218359L3 Lenti ORF clone of Gpat2 (Myc-DDK-tagged) - Mouse glycerol-3-phosphate acyltransferase 2, mitochondrial (Gpat2), nuclear gene encoding mitochondrial protein 10 ug
$1,032.00
MR218359L4 Lenti ORF clone of Gpat2 (mGFP-tagged) - Mouse glycerol-3-phosphate acyltransferase 2, mitochondrial (Gpat2), nuclear gene encoding mitochondrial protein 10 ug
$1,032.00

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