Arhgef19 (NM_172520) Mouse Untagged Clone

CAT#: MC221729

Arhgef19 (untagged) - Mouse Rho guanine nucleotide exchange factor (GEF) 19 (Arhgef19), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Arhgef19
• Synonyms: 6030432F23; 6430573B13Rik; WGEF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221729 representing NM_172520
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTGTGGGCCACCTGCAACCCTCCAGCCTCACTTGGCTGGACCACCTGGCACCGCCCGTCGCCCAG
  TAGCTGTGTGCCAGCAGGAGAGCCTGTCCTTCGTGGAGCTGCCTCCGAGCCGACTGTGTGTGGATCTCTT
  TCCCATTGCTCCAGAGGAGCTGCAAGCTCCCGGTAGCCGCTGGTCCCTGGCAACCCCTGCCCCTCTCCAA
  GGACTGTTATGGCCGACGTCCCCAGGAGGCCCAGATACCCATATCGCCTCCACCAGTGGAGGAATGAGAC
  CCGGCAGGGCTGGCAGCTGGCCTCACTGTCCTGGTGCCCAGCCCCCAACTCTGGTGGAACCGTGGAGTTC
  TCAGCACATGCAGCCCCAACGCAGGGCCAGCCACAGTGGCTCAGAGAAGTCAGCCTGGCGCAAGATGCAG
  GTGTATCATAACGAAGAGGCCACCGGCACCGAGACCCCTACGGGCCTTCTGGAGACTGGCCAGGCTGCGC
  AAGAGCAGGCTCTGTGTGCTCTAGAGCCCGGGCCCCAGGAGCTTTCTGGGAACGCCAGAGGGGGCCTTGA
  GGCACCGGAGCGAAGGCGTTTCTCGGCCTCAGAGCTGATGACTAGGCTGCATTCCTCTCTGCGCTTGGGG
  CGCAGCACTGCCACCAGGGTTCTGACCTCGGGCTCAGGTGCTGGAGCCATCCGGGAAGGGAAACTGCCCG
  TGAGGGAATCCCGAAGAGCCGAGACAAGGGCGGACAGTGTGTCGCTTCCAGGACCCGTCGATCTAAACGA
  GGTCCACGGTGGTCTGGTGGAACCCAGGCTGGACACGCAAGACAAGCCGACTCAGGATCCCAGCAGCGCC
  ACTGAGAGGCGCCAGTCCCGCTTCCTCCTTAATTCTGTCCTCTACCAGGAGTACAGCGACGTGGCCAGCG
  CGCGCGAGTTGCGGCGGCAGCAGCGTGAGGAGGAGGGCCCCGGGGACGGAGCGGAGGGCGCGGAGGAGGG
  GCCCGGGCCACCGCGCGCCAACCTCTCCCCGAGCAGCTCTTTCCGCGCGCAACGCTCGACGCGGGGCTCC
  ACCTTCTCGCTGTGGCAGGACATCCCCGACGTGCGCGGCAGTGGCGTCCTGGCCACGCTGAGCCTGCGCG
  ACTGCAAGCTGCAGGAGGCCAAATTTGAGCTGATTACCTCGGAGGCCTCCTACATCCATAGCCTGTCGGT
  GGCCGTGGGTCACTTCTTAGGTTCCGTGGAGCTGAGCGAGTGTCTGGGGACTCAGGACAAGCAGTGGCTA
  TTTTCAAAACTGCCCGAGGTCAAGAGCACCAGTGAGAGGTTCCTTCACGACCTGGAGCAGCGTCTAGAGG
  CCGATGTGCTGCGCTTCAGCGTGTGCGATGTGGTTCTACACCATTGCCCCGCATTCCGCCGAGTCTACCT
  GCCTTACGTCACCAACCAGGCCTACCAGGAGCGCACCTACCAGCGCCTGCTCCTAGAGAACCCCAAGTTC
  CCCGGCATCCTGGCTCGCCTAGAGGAGTCTCCTGTGTGCCAGCGGCTGCCTCTCACCTCCTTCCTCATCT
  TACCGTTCCAGAGGGTTACCCGCCTCAAGATGCTGGTAGAGAACATTTTGAAGCGGACAGCACCGGGCTC
  CCAAGATGAGGACATGGCCACCAAGGCCTTCAGCGCTCTAAAGGAGCTGGTGCAGGAGTGCAACGCGAGC
  GTGCAGTCCATGAAGCGGACGGAGGAACTAATTCACCTAAGCAAGAAGATCCATTTTGAGGGCAAGATCT
  TCCCGCTGATCTCACAGGCCCGCTGGCTGGTCCGGCACGGGGAGCTGGTGGAGTTGGCCCCCCTGCCCGC
  CGCACCGCCAGCCAAACTGAAGCTGTCCAGCAAGGCCGTGTACCTTCACCTCTTCAATGACTGCCTGCTG
  CTGTCCCGGAGGAAGGAGCTCGGGAAGTTTGCCGTTTTTGTCCATGCCAACATGGCTGAGCTACAGGTTC
  GAGACCTCAGCCTGAAGCTCCAGGGCATCCCAGGCCATGTGTTCCTGCTCAGGCTGCTGCACGGGCAGCG
  CGCCAGGCACCAGCTGCTGCTGAGAGCACGGACCGAAAGTGAGAAGCAACGGTGGATTTCAGCCTTGAGA
  CCATCCAGCCCCCAGGAGGACAAGGAGATCACATGTGATGGGGAAGACCGACCCCAGGTCCAGTGTGTGA
  GGACCTACAAGGCCCTGCAGCCAGATGAACTCACCTTGGAGAAGACAGACATCCTGGCAGTGAAGACCAG
  GACCAGCGATGGCTGGCTGGAGGGCGTGCGCCTGGCCGATGGTGAGAAGGGATGGGTGCCTCAGGCCCAT
  GTGGTGGAGATCAGCAGCCTCAGCGCTCGACTGCGCAACCTCAGAGAGTACAAGAGGGTCTCCAACGCCA
  GCAGCAAGCTGGGGGACCCACCTGCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_172520
• Insert Size: 2409 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172520.2, NP_766108.1
• RefSeq Size: 3397 bp
• RefSeq ORF: 2409 bp
• Locus ID: 213649
• UniProt ID: Q8BWA8
• Cytogenetics: 4 D3
• Gene Summary: Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase.[UniProtKB/Swiss-Prot Function]