Pde10a (NM_011866) Mouse Untagged Clone

CAT#: MC221706

Pde10a (untagged) - Mouse phosphodiesterase 10A (Pde10a), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pde10a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221706 representing NM_011866
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGATGGACCCTCTAACAATGCGAGTTGCTTCCGAAGGCTGACCGAGTGTTTCCTCAGCCCCAGTT
  TGACGGATGAAAAGGTGAAGGCCTATCTTTCTCTCCATCCCCAGGTATTAGATGAATTTGTTTCTGAAAG
  TGTTAGTGCAGAGACTGTGGAAAAGTGGCTGAAGAGGAAAACCAACAAAGCAAAAGATGAACCATCTCCC
  AAGGAAGTCAGCAGGTACCAGGATACGAATATGCAGGGAGTCGTGTACGAGCTGAACAGCTACATAGAGC
  AGCGCCTGGACACGGGCGGGGACAACCACCTGCTCCTCTATGAGCTCAGCAGCATCATCAGGATAGCCAC
  AAAAGCCGACGGATTTGCACTGTACTTCCTTGGAGAGTGCAATAATAGCCTGTGTGTGTTCATACCACCC
  GGGATGAAGGAAGGCCAACCCCGGCTCATCCCTGCAGGGCCCATCACCCAGGGTACCACCATCTCTGCCT
  ACGTGGCCAAGTCTAGGAAGACGTTGTTGGTAGAGGATATCCTTGGGGATGAGCGATTTCCTCGAGGTAC
  TGGCCTGGAATCAGGAACCCGCATCCAGTCTGTTCTTTGCTTGCCCATTGTCACTGCCATTGGAGACTTG
  ATTGGCATCCTTGAACTGTACAGGCACTGGGGCAAAGAGGCCTTCTGCCTCAGCCATCAGGAGGTTGCAA
  CAGCCAATCTTGCTTGGGCTTCCGTAGCAATACACCAGGTGCAGGTGTGTAGAGGTCTCGCCAAACAGAC
  CGAACTGAATGACTTCCTACTCGACGTATCAAAGACATACTTTGATAACATAGTTGCCATAGACTCTCTA
  CTTGAACACATCATGATATATGCAAAAAATCTAGTGAACGCCGACCGCTGCGCGCTCTTCCAGGTGGACC
  ACAAGAACAAGGAGCTGTACTCGGACCTGTTTGACATTGGGGAGGAGAAGGAGGGGAAGCCCATCTTCAA
  GAAGACCAAGGAGATCAGATTTTCCATTGAGAAAGGGATTGCTGGTCAAGTGGCAAGAACAGGCGAAGTC
  TTGAACATTCCCGATGCCTACGCGGACCCTCGCTTTAACAGGGAGGTGGACCTGTACACAGGCTACACCA
  CGAGGAACATTCTGTGTATGCCCATAGTGAGCCGAGGCAGCGTGATTGGCGTGGTGCAGATGGTGAACAA
  GATCAGCGGTAGCGCCTTCTCCAAGACAGACGAGAACAACTTCAAGATGTTTGCTGTCTTCTGCGCACTG
  GCCTTGCACTGTGCTAACATGTACCACAGGATCCGCCACTCAGAATGCATCTACAGGGTTACCATGGAGA
  AGCTTTCCTACCACAGCATCTGCACCTCCGAGGAGTGGCAAGGCCTCATGCGCTTCAACCTACCAGCACG
  CATCTGCCGGGACATCGAGCTATTCCACTTTGACATTGGTCCTTTCGAGAACATGTGGCCTGGGATCTTT
  GTCTACATGATCCATCGGTCTTGTGGGACATCCTGTTTTGAACTTGAAAAATTGTGCCGTTTTATCATGT
  CTGTGAAGAAGAACTATCGGCGGGTTCCTTACCACAACTGGAAGCATGCAGTCACGGTGGCACACTGCAT
  GTATGCCATACTTCAAAACAACAATGGCCTCTTCACAGACCTCGAGCGCAAAGGCCTGCTAATTGCGTGT
  CTGTGCCATGACCTGGACCACAGGGGCTTCAGTAACAGCTACCTGCAGAAGTTCGACCACCCCCTGGCGG
  CGCTGTACTCCACCTCCACCATGGAGCAACACCACTTCTCCCAGACGGTGTCCATCCTTCAGCTGGAAGG
  GCACAATATCTTCTCCACCCTGAGCTCCAGCGAGTACGAGCAGGTGCTGGAGATCATCCGCAAAGCCATC
  ATCGCCACCGACCTCGCCCTATACTTTGGGAACAGGAAGCAGTTGGAGGAGATGTACCAGACAGGGTCGC
  TGAACCTCCACAACCAGTCCCATCGAGACCGTGTCATCGGCTTGATGATGACTGCCTGTGATCTTTGCTC
  TGTGACCAAACTATGGCCAGTTACAAAATTGACAGCGAATGATATATATGCAGAATTCTGGGCTGAGGGT
  GATGAGATGAAGAAGCTGGGCATACAGCCCATTCCTATGATGGACAGAGACAAGCGAGATGAAGTCCCTC
  AAGGGCAGCTCGGATTCTACAATGCTGTGGCCATTCCCTGCTATACCACCTTGACGCAGATCCTCCCACC
  CACAGAGCCTCTGCTGAAGGCCTGCAGGGATAACCTCAATCAGTGGGAGAAGGTAATTCGCGGGGAAGAG
  ACAGCAATGTGGATTTCAGGCCCAGGCCCGGCGCCTAGCAAGAGCACACCTGAGAAGCTGAACGTGAAGG
  TTGAAGACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_011866
• Insert Size: 2391 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011866.2, NP_035996.2
• RefSeq Size: 7717 bp
• RefSeq ORF: 2391 bp
• Locus ID: 23984
• UniProt ID: Q8CA95
• Cytogenetics: 17 A1
• Gene Summary: Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. Can hydrolyze both cAMP and cGMP, but has higher affinity for cAMP and is more efficient with cAMP as substrate. May play a critical role in regulating cAMP and cGMP levels in the striatum, a region of the brain that contributes to the control of movement and cognition.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) contains an alternate exon in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon compared to variant 1. The resulting protein (isoform 2) has a shorter N-terminus compared to isoform 1.