Rasgrp1 (NM_011246) Mouse Untagged Clone

CAT#: MC221698

Rasgrp1 (untagged) - Mouse RAS guanyl releasing protein 1 (Rasgrp1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rasgrp1
• Synonyms: Rasgrp
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221698 representing NM_011246
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAACCCTGGGCAAGGCGAGAGAGGCTCCGCGGAAACCTTGCCATGGCTCCAGAGCTGGCCCCAAAG
  CAAGACTAGAGGCCAAATCAACCAACAGCCCTCTCCCTGCCCAGCCCAGCTTGGCCCAGATCACCCAGTT
  CCGAATGATGGTGTCCCTGGGACATTTGGCCAAAGGAGCCAGCCTGGATGATCTTATTGACAGCTGCATT
  CAATCTTTTGATGCGGATGGAAACCTGTGTCGGAATAACCAACTGTTACAAGTCATGCTAACCATGCACC
  GGATCATCATCTCCTCAGCCGAGCTGCTACAAAAAGTCATGAATCTATATAAGGATGCCCTGGAAAAGAA
  TTCTCCAGGAGTTTGCCTGAAGATCTGCTATTTTGTCAGGTATTGGATAACAGAATTCTGGATCATGTTC
  AAGATGGATGCCAGCTTGACCAGCACCATGGAAGAGTTCCAAGACCTGGTGAAAGCCAATGGTGAGGAGA
  CCCACTGCCACCTCATCGACACGACCCAAATTAATTCTCGAGACTGGTCCAGGAAACTGACTCAGAGGAT
  AAAATCGAATACTAGCAAGAAGCGCAAAGTGTCCCTGCTGTTTGACCATCTGGAACCTGAAGAACTGTCT
  GAACACCTCACCTACCTTGAGTTCAAGTCCTTCCGACGGATATCTTTCTCTGATTACCAAAATTACCTTG
  TAAATAGCTGCGTAAAAGAGAACCCCACCATGGAGAGGTCCATTGCCCTGTGCAATGGCATCTCCCAGTG
  GGTACAACTGATGGTTCTCAGCCGTCCCACCCCGCAGCTCCGGGCAGAGGTCTTCATCAAGTTCATCCAT
  GTGGCTCAGAAACTCCACCAGCTACAGAACTTCAACACGCTGATGGCTGTGATCGGGGGACTGTGTCACA
  GCTCCATCTCCAGGCTTAAGGAAACAAGTTCACATGTCCCACATGAGATCAATAAGGTTCTGGGCGAGAT
  GACTGAACTGCTGTCCTCCTGCAGAAACTATGACAACTACAGGCGAGCCTATGGGGAGTGCACCCACTTC
  AAAATCCCCATACTGGGTGTGCACCTCAAGGACCTCATATCCCTGTATGAGGCTATGCCCGACTATCTGG
  AAGATGGGAAGGTGAATGTCCAAAAGCTCCTGGCCCTTTACAATCATATCAATGAATTGGTCCAGCTGCA
  GGAGATGGCCCCACCATTGGATGCCAACAAGGACTTGGTGCATCTGCTGACGTTATCCCTGGATCTATAC
  TATACAGAAGATGAAATCTATGAGCTTTCCTATGCCCGGGAGCCGAGGAACCACAGAGCTCCGCCACTGA
  CACCTTCGAAGCCACCAGTTGTAGTAGACTGGGCCTCTGGAGTGTCTCCCAAACCTGACCCAAAGACCAT
  CAGCAAACACGTCCAAAGGATGGTGGATTCTGTCTTTAAGAACTATGATCTCGACCAGGATGGCTATATC
  TCTCAGGAGGAGTTTGAGAAGATCGCTGCAAGCTTTCCATTTTCCTTCTGTGTGATGGACAAAGATAGGG
  AAGGCCTCATCAGCAGAGACGAGATCACGGCCTACTTCATGAGGGCCAGCTCCATCTATTCCAAGCTGGG
  CCTGGGCTTTCCACACAACTTTCAAGAAACCACTTACCTGAAGCCCACCTTCTGTGACAACTGTGCTGGC
  TTTCTCTGGGGCGTGATCAAGCAAGGCTATCGCTGTAAAGACTGCGGGATGAACTGCCACAAACAGTGCA
  AAGACCTGGTGGTGTTCGAGTGCAAGAAGCGAATCAAGAGCCCAGCAATATCCACAGAGAACATCAGCTC
  TGTGGTACCAATGTCCACCCTTTGTCCACTGGGAACCAAAGATCTGCTCCATGCACCCGAAGAAGGATCT
  TTCATTTTCCAAAACGGAGAGATTGTGGACCACAGTGAGGAGAGCAAGGATAGGACCATCATGCTCCTCG
  GAGTGTCCTCCCAGAAAATTTCAGTTCGGCTGAAGAGGACTGTTGCCCACAAGAGCACCCAAACAGAATC
  GTTCCCGTGGGTTGGTGGCGAGACGACCCCTGGTCACTTTGTGCTGTCTTCTCCAAGGAAGTCGGCGCAG
  GGCGCTCTTTATGTGCACAGTCCAGCATCTCCATGCCCCAGCCCAGCACTGGTCCGAAAGCGGGCATTCG
  TCAAGTGGGAGAACAAAGAGTCCCTTATAAAACCAAAACCAGAACTTCACCTCCGGCTCCGGACCTACCA
  AGAACTGGAACAGGAAATAAATACCCTGAAAGCAGATAACGATGCTCTGAAGATCCAACTGAAGTACGCA
  CAGAAGAAAATAGAATCCCTGCAGCTTGGGAAAAGCAATCATGTCTTAGCCCAGATGGACCACGGTGATA
  GTGCTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_011246
• Insert Size: 2388 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011246.2, NP_035376.1
• RefSeq Size: 5155 bp
• RefSeq ORF: 2388 bp
• Locus ID: 19419
• UniProt ID: Q9Z1S3
• Cytogenetics: 2 59.19 cM
• Gene Summary: Functions as a calcium- and diacylglycerol (DAG)-regulated nucleotide exchange factor specifically activating Ras through the exchange of bound GDP for GTP. Activates the Erk/MAP kinase cascade. Regulates T-cell/B-cell development, homeostasis and differentiation by coupling T-lymphocyte/B-lymphocyte antigen receptors to Ras. Regulates NK cell cytotoxicity and ITAM-dependent cytokine production by activation of Ras-mediated ERK and JNK pathways (By similarity). Functions in mast cell degranulation and cytokine secretion, regulating FcERI-evoked allergic responses (PubMed:17190838). May also function in differentiation of other cell types. Proto-oncogene, which promotes T-cell lymphomagenesis when its expression is deregulated (PubMed:15829980, PubMed:17210708).[UniProtKB/Swiss-Prot Function]