Fgfr4 (NM_008011) Mouse Untagged Clone

CAT#: MC221681

Fgfr4 (untagged) - Mouse fibroblast growth factor receptor 4 (Fgfr4), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Fgfr4
• Synonyms: Fgfr-4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221681 representing NM_008011
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGCTGCTCTTGGCCCTGTTGAGCATCTTTCAGGGGACACCAGCTTTGTCCCTTGAGGCCTCTGAGG
  AAATGGAGCAGGAGCCCTGCCTAGCCCCAATCCTGGAGCAGCAAGAGCAGGTGTTGACGGTGGCCCTGGG
  GCAGCCTGTGAGGCTGTGCTGTGGGCGCACCGAGCGTGGTCGTCACTGGTACAAAGAGGGCAGCCGCCTA
  GCATCTGCTGGGCGAGTACGGGGTTGGAGAGGCCGCCTGGAGATCGCCAGCTTCCTTCCTGAGGATGCTG
  GCCGATACCTCTGCCTGGCCCGTGGCTCCATGACCGTCGTACACAATCTTACGTTGCTTATGGATGACTC
  CTTAACCTCCATCAGTAATGATGAAGACCCCAAGACACTCAGCAGCTCCTCGAGTGGTCATGTCTACCCA
  CAGCAAGCACCCTACTGGACACACCCCCAACGCATGGAGAAGAAACTGCATGCAGTGCCTGCCGGGAATA
  CTGTCAAATTCCGCTGTCCAGCTGCAGGGAACCCCATGCCTACCATCCACTGGCTCAAGGATGGACAGGC
  CTTCCACGGGGAGAATCGTATTGGAGGCATTCGGCTGCGCCACCAACACTGGAGCCTGGTGATGGAAAGT
  GTGGTACCCTCGGACCGTGGCACATACACATGCCTTGTGGAGAACTCTCTGGGTAGCATTCGCTACAGCT
  ATCTCCTGGATGTGCTGGAGCGGTCCCCGCACCGGCCCATCCTGCAGGCGGGGCTCCCAGCCAACACCAC
  AGCTGTGGTTGGCAGCGATGTGGAGCTACTCTGCAAGGTGTACAGCGACGCCCAGCCCCACATACAGTGG
  CTGAAACACGTCGTCATCAACGGCAGCAGCTTCGGCGCCGACGGTTTCCCCTACGTACAAGTCCTGAAGA
  CAACAGACATCAATAGCTCGGAGGTAGAGGTCTTGTATCTGAGGAACGTGTCCGCTGAGGATGCAGGAGA
  GTATACCTGTCTGGCGGGCAACTCCATCGGCCTTTCCTACCAGTCAGCGTGGCTCACGGTGCTGCCAGAG
  GAAGACCTCACGTGGACAACAGCAACCCCTGAGGCCAGATACACAGATATCATCCTGTATGTATCAGGCT
  CACTGGTTCTGCTTGTGCTCCTGCTGCTGGCCGGGGTGTATCATCGGCAAGTCATCCGTGGCCACTACTC
  TCGCCAGCCTGTCACTATACAAAAGCTGTCCCGTTTCCCTTTGGCCCGACAGTTCTCTTTGGAGTCGAGG
  TCCTCTGGCAAGTCAAGTTTGTCCCTGGTGCGAGGTGTCCGTCTCTCCTCCAGCGGCCCGCCCTTGCTCA
  CGGGCCTTGTGAATCTAGACCTGCCTCTCGATCCGCTTTGGGAATTCCCCCGGGACAGGTTGGTGCTCGG
  AAAGCCCCTGGGTGAGGGCTGCTTTGGGCAAGTGGTTCGTGCAGAGGCCTTTGGTATGGATCCCTCCCGG
  CCCGACCAAACCAGCACCGTGGCTGTGAAGATGCTGAAAGACAATGCCTCCGACAAGGATTTGGCAGACC
  TGGTCTCCGAGATGGAGGTGATGAAGCTAATCGGAAGACACAAGAACATCATCAACCTGCTGGGTGTCTG
  CACTCAGGAAGGGCCCCTGTACGTGATTGTGGAATGTGCCGCCAAGGGAAACCTTCGGGAATTCCTCCGT
  GCCCGGCGCCCCCCAGGCCCTGATCTCAGCCCTGATGGACCTCGGAGCAGCGAAGGACCACTCTCCTTCC
  CGGCCCTAGTCTCCTGTGCCTACCAGGTGGCCCGAGGCATGCAGTATCTGGAGTCTCGGAAGTGCATCCA
  CCGGGACCTGGCTGCCCGAAATGTGCTGGTGACCGAGGATGATGTGATGAAGATCGCTGACTTTGGGCTG
  GCACGTGGTGTCCACCACATTGACTACTATAAGAAAACCAGCAACGGCCGCCTGCCAGTCAAATGGATGG
  CTCCAGAGGCATTGTTCGACCGCGTGTACACACACCAGAGTGACGTGTGGTCTTTCGGGATCCTGCTGTG
  GGAAATCTTCACCCTCGGGGGCTCCCCATACCCTGGCATTCCGGTGGAGGAGCTCTTCTCACTGCTGCGA
  GAGGGGCACAGGATGGAGCGGCCCCCAAACTGCCCCTCAGAGCTGTATGGGCTAATGAGGGAGTGCTGGC
  ACGCAGCCCCATCTCAGAGGCCTACTTTTAAGCAGCTGGTGGAAGCTCTGGACAAGGTCCTGCTGGCTGT
  CTCTGAAGAGTACCTTGACCTCCGCCTGACCTTTGGACCCTTTTCTCCCTCCAATGGGGATGCCAGCAGC
  ACCTGCTCCTCCAGTGACTCGGTTTTCAGCCACGACCCTTTGCCCCTCGAGCCAAGCCCCTTCCCTTTCT
  CTGACTCGCAGACGACATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008011
• Insert Size: 2400 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008011.2, NP_032037.2
• RefSeq Size: 3146 bp
• RefSeq ORF: 2400 bp
• Locus ID: 14186
• UniProt ID: Q03142
• Cytogenetics: 13 29.8 cM
• Gene Summary: Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays a role in the regulation of cell proliferation, differentiation and migration, and in regulation of lipid metabolism, bile acid biosynthesis, glucose uptake, vitamin D metabolism and phosphate homeostasis. Required for normal down-regulation of the expression of CYP7A1, the rate-limiting enzyme in bile acid synthesis, in response to FGF19. Phosphorylates PLCG1 and FRS2. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Promotes SRC-dependent phosphorylation of the matrix protease MMP14 and its lysosomal degradation. FGFR4 signaling is down-regulated by receptor internalization and degradation; MMP14 promotes internalization and degradation of FGFR4. Plays a role in postnatal lung development. May be involved in the development of skeletal muscle cell lineages.[UniProtKB/Swiss-Prot Function]