Whamm (NM_001004185) Mouse Untagged Clone

CAT#: MC221677

Whamm (untagged) - Mouse WAS protein homolog associated with actin, golgi membranes and microtubules (Whamm), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Whamm
• Synonyms: BB081391; mKIAA1971; Whdc1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221677 representing NM_001004185
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACAGCGAGCAGCCCGACAGCCTGGATGGCTGGGTTCCGCTGCGTGAGGACCTCTTCCCGGAGCCCG
  AGCGCCATCAGCTGCGTTTCCTGGTGGCCTGGAACGCCGCGAAGGGCCAGTTCGCCGTCACCTGTCACGA
  CCGCACGGCGCAGCGGCGGCGGCGCGAGCGGCGGGAGGCCGGCGACGGGGGATGTAGCTGGGCCGGGGTG
  CTGTCGCCCGCCGGGTTCCGCGGCGCGCACCGGCAGCTGGCGGCGCTGTGGCCGGCCCTGGAGCCCTGCT
  TCCCGCCGCTGCCGCCCGAGCTGGACGCGGCGAGCGGCGCGGGCTGGGGCCTGGGACGCGGGCTGTGGGC
  CCTCCTGTGGCCGCTCCTGTGGCCGGCGCCTGCGGACCCTGGCGACTCGGCGCTGCAGGAGCTGTGCCGG
  CAGCTGGAGCACTACCTGGGCCTGGCGGCCGAAGGATGCGGCGGCGCCACCGTGCGCGACGTGCTCTTCC
  CGGCCCCGGGTGACTCGGCAGACTGCGAGGGCCTGAGCGAGTTCCGCGAGAGGACGCTGCGCGCTCGCCT
  GGGCCAAACGGCCACCCGGCTGCACCAGGTTCTTCAAGATCACGGGAAAGCCAATACCATGGTAGCATTA
  ATGAAAGTTTATCAAGAGGAAGATGAGCTCTATCAAGAATTAGTTACCATGGCAACCACATTCTTCCAGT
  ATTTACTGCAGCCATTTAGAGACATGCGAGAAGTTGCAACTTCATGTAAGCTTGGTATTTTGAAGTCCCT
  GGATGAGGATGAGCTGGGTCCTAGAAGGGTGGCTGCCCTACAGAAGGAAGCCAGTGAATGGACCAGACAG
  GCTGAAGAAGCAGTGGGCTCTATCCAAGACATCACTGTGAATTATTTTAAAGAAACAGTAACAGCACTAA
  CAGGAATGCAGAAACAAATGGAGCAGGATCAGAAGAGATTTGGCCAGGCTGCCTGGGCCACAGCCATGCC
  CAGGCTAGAAAATCTCAAGCTCATGTTAGCTCGGGAGACTCTGCAGCTTATGAGAGCCAAAGAGCTGTGT
  CTAAAACACAGGCAAGCTGAAATCCAGAGAAAGGTGGAAGATCTTCCGCGCCAAGGAAAGCAGTTAGATG
  TTGTGGATGAGCTGGAGATACAGTGTTATGAGATCCAGTTGGAGTTGTATGATGTCAAGCTTGAGATGCT
  GAGAAATGAGGAGACAATCCTTGTCACGCGGTTGGACTCTGTTAAGAGACTTATAACAGAGAAGCAGGCT
  GAAGTGATCTACTACGACCCGTGTGAGAGCCCCGAGGAGCTGCAGAGCCTGGCTCCTGACCTGGAACTGC
  ACCTTGGTGACAACAGAGAGCTGAGAGCACTCAGCCAGCAGTGCCAGCGGCTGGAGGCTCAGCGGGGCCG
  CATCTGCTCCAGGAGAGCCCTCCTTAGGAACAGAAAGGATCACTGCCGAGAAAATCATCAGCTCAGACTG
  CAGCAGGCCAAACAAAGTCTGAGACACCTTCACCAGCACCACAGTATTCAAATGAAGAGAGACAAAGTAA
  AAGAGGAAGAACAAAAGAAAAAAGAATGGATAGACCATGAACGCCAGAAAACCCTTGAGCGGCTAAGAGC
  ATATAAGGAGAAGTGCCCGGCTCATCGGTCTGCACTGAAAACAACTTGTTCAGAGTCCATGGTGTCAAAC
  CTGCCAGGTGGGCGCTCTCAGAAGAGGCTCTCAACGGCTCACCACCACAAGACAGCTCATCCTGCCTCCA
  GCAAAACCGGAAGTGCTGTTCCCTTACCTGAAGCCAGTGTGAGACCCCCTGAGCATCAAGACCCCTGTGG
  CAGTGTCCCTGTCCAGGCTTTTGTTCCTGTTAGTGACCAGACACTGTCTGGATCCAGTGAAGATCTGTCA
  CTACCACCACAGCCACCAGCTCCTCCTCTCCCTCCTCCTCCTCCCCCTCCTCCCCCTCCTCCTCTTCCCC
  CTGCTCTGTCCTCTTTTCAAGGTACCACTCATCAGAATTTAGGGCTCAGGACTTTGGCAACAGAGGACCG
  GCCACTCCCACTAGCGTGTGACCCATCTGCTGGGAGACCATGTGACTACCAGGGTCCAGGCTCTATGGAT
  GAAGTGCTGGCCTCTCTCCGGCAGGGCAAAGCCTCTCTCCGGAAAGTAGAAACGCCCACCTTGCCCCATC
  CTGGCACCTCAGTCAATGAACAAGTTCTGGCTGCCATCAGACAGGGCGTGCAGCTGAAGAAAGTCCACAC
  GGGCCAAGGTGTGGATCCCGGCAAGAAGAGCACCAGCGACCTGGAGAGGAGCATCAGAGAAGCCCTGGAG
  AGAATCAAGAAAGTTTCTGCCGACTCAGAGGAGGACAATGATGAGCCGAGCCCCACTGAGTGGGACCGCT
  AG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001004185
• Insert Size: 2382 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001004185.3, NP_001004185.3
• RefSeq Size: 3069 bp
• RefSeq ORF: 2382 bp
• Locus ID: 434204
• UniProt ID: Q571B6
• Cytogenetics: 7 D3
• Gene Summary: Acts as a nucleation-promoting factor (NPF) that stimulates Arp2/3-mediated actin polymerization both at the Golgi apparatus and along tubular membranes. Involved as a regulator of Golgi positioning and morphology. Its activity in membrane tubulation requires F-actin and interaction with microtubules. Proposed to use coordinated actin-nucleating and microtubule-binding activities of distinct WHAMM molecules to drive membrane tubule elongation; when MT-bound can recruit and remodel membrane vesicles but is prevented to activate the Arp2/3 complex. Required for RhoD-dependent actin reorganization such as in cell adhesion and cell migration (By similarity). Participates in vesicle transport between the reticulum endoplasmic and the Golgi complex.[UniProtKB/Swiss-Prot Function]