Smo (NM_176996) Mouse Untagged Clone

CAT#: MC221670

Smo (untagged) - Mouse smoothened homolog (Drosophila) (Smo), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Smo
• Synonyms: bnb; E130215L21Rik; Smoh; smoothened
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221670 representing NM_176996
  Red=Cloning site Blue=ORF
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGCTGGCCGCCCCGTGCGTGGGCCCGAGCTGGCGCCCCGGAGGCTGCTGCAGTTGCTTCTGCTGG
  TACTGCTGGGGGGCCCGGGCCGGGGGGCGGCCTTGAGCGGGAACGTGACCGGGCCTGGGCCTCACAGCGC
  CAGCGGGAGCTCGAGGAGGGACGTGCCGGTGACCAGCCCTCCGCCGCCGCTGCTGAGCCACTGCGGCCGG
  GCCGCCCACTGCGAGCCTTTGCGCTACAACGTGTGCCTGGGCTCGGCGCTGCCCTACGGAGCCACCACCA
  CGCTGCTGGCTGGGGACTCGGACTCGCAGGAGGAAGCGCACGGCAAGCTCGTGCTCTGGTCCGGCCTCCG
  GAATGCCCCCCGCTGCTGGGCAGTGATCCAGCCCCTGCTGTGTGCTGTCTACATGCCCAAGTGTGAGAAT
  GACCGAGTGGAGTTGCCCAGCCGTACCCTCTGCCAGGCCACCCGAGGCCCCTGTGCCATTGTGGAGCGGG
  AGCGAGGGTGGCCTGACTTTCTGCGTTGCACACCGGACCACTTCCCTGAAGGCTGCCCAAACGAGGTACA
  AAACATCAAGTTCAACAGCTCAGGCCAATGTGAAGCACCCTTGGTGCGAACAGACAACCCCAAGAGCTGG
  TATGAGGACGTGGAGGGCTGTGGGATTCAGTGTCAGAACCCGCTGTTCACCGAGGCCGAGCACCAGGACA
  TGCACAGCTACATCGCAGCCTTCGGGGCGGTCACTGGTCTCTGCACGCTCTTCACACTGGCCACCTTTGT
  GGCTGACTGGCGGAACTCCAATCGCTACCCTGCGGTTATTCTCTTCTATGTCAATGCGTGTTTCTTCGTG
  GGCAGCATTGGCTGGCTGGCCCAGTTCATGGATGGTGCCCGCCGAGAGATTGTTTGCCGAGCAGATGGCA
  CCATGAGATTTGGGGAGCCCACCTCCAGTGAGACCCTGTCCTGTGTCATCATCTTTGTCATTGTGTACTA
  TGCCTTGATGGCTGGAGTAGTCTGGTTCGTGGTCCTCACCTATGCCTGGCACACCTCCTTCAAAGCCCTG
  GGCACCACCTACCAGCCTCTCTCGGGCAAGACATCCTATTTCCACCTGCTCACGTGGTCACTCCCCTTTG
  TCCTCACGGTGGCAATCCTGGCTGTGGCTCAGGTAGATGGAGACTCCGTGAGTGGCATCTGTTTTGTAGG
  CTACAAGAACTATCGGTACCGTGCTGGCTTTGTCCTGGCCCCAATTGGCCTGGTGCTTATTGTGGGAGGC
  TACTTCCTCATCAGAGGGGTCATGACTCTGTTCTCCATCAAGAGCAACCACCCTGGGCTTCTGAGTGAGA
  AGGCAGCCAGCAAGATCAACGAGACCATGCTGCGCCTGGGCATTTTTGGCTTCCTGGCCTTTGGCTTTGT
  GCTCATCACCTTCAGCTGCCACTTCTATGACTTCTTCAACCAGGCTGAGTGGGAGCGTAGCTTCCGGGAC
  TATGTGCTATGCCAAGCCAACGTGACCATCGGGCTGCCTACCAAGAAGCCCATTCCTGACTGTGAGATCA
  AGAATCGGCCCAGCCTCCTGGTGGAGAAGATCAATCTATTTGCCATGTTTGGCACTGGCATTGCCATGAG
  CACCTGGGTCTGGACCAAGGCCACCCTGCTCATCTGGAGGCGCACCTGGTGCAGGTTGACTGGGCACAGT
  GATGATGAGCCCAAGAGAATCAAGAAGAGCAAGATGATCGCCAAGGCCTTCTCTAAGCGGCGTGAGCTGC
  TGCAGAACCCGGGCCAGGAGCTCTCCTTCAGCATGCACACTGTCTCCCATGATGGACCTGTTGCGGGTTT
  GGCTTTTGACCTCAATGAACCCTCAGCTGACGTCTCCTCTGCCTGGGCTCAGCATGTCACCAAGATGGTG
  GCTCGGAGAGGAGCCATATTGCCCCAGGATGTGTCCGTTACCCCTGTGGCAACTCCAGTGCCACCAGAAG
  AACAAGCCAACATGTGGCTGGTTGAGGCAGAGATCTCCCCAGAGTTAGAGAAGCGTTTGGGCCGGAAGAA
  AAAGCGGAGGAAGAGGAAGAAGGAGGTGTGCCCCTTGAGGCCAGCCCCTGAGCTTCACCACTCTGCCCCT
  GTTCCTGCCACCAGTGCAGTTCCTCGGCTGCCTCAGCTGCCTCGGCAGAAGTGCCTGGTAGCTGCAAACG
  CCTGGGGAACAGGGGAGTCCTGCCGACAGGGAGCCTGGACTCTAGTCTCCAACCCCTTCTGCCCAGAGCC
  TAGTCCCCATCAAGATCCATTTCTCCCTGGTGCCTCAGCCCCCCGGGTCTGGGCTCAGGGCCGCCTCCAG
  GGGCTGGGATCCATTCATTCCCGCACTAACCTAATGGAGGCTGAGATCTTGGATGCAGACTCGGACTTCT
  GA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_176996
• Insert Size: 2382 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC096028, AAH96028
• RefSeq Size: 3542 bp
• RefSeq ORF: 2382 bp
• Locus ID: 319757
• UniProt ID: P56726
• Cytogenetics: 6 12.36 cM
• Gene Summary: G protein-coupled receptor that probably associates with the patched protein (PTCH) to transduce the hedgehog's proteins signal. Binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO) (By similarity). Required for the accumulation of KIF7, GLI2 and GLI3 in the cilia. Interacts with DLG5 at the ciliary base to induce the accumulation of KIF7 and GLI2 at the ciliary tip for GLI2 activation (PubMed:25644602).[UniProtKB/Swiss-Prot Function]