Disc1 (NM_174853) Mouse Untagged Clone

CAT#: MC221665

Disc1 (untagged) - Mouse disrupted in schizophrenia 1 (Disc1), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Disc1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221665 representing NM_174853
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGGGCGGGGGTCCCCGGGGCGCTCCGATCCACAGTCCGAGCCACGGCGCAGACAGTGGGCATGGCT
  TACCGCCTGCAGTAGCCCCTCAGAGGCGGCGGCTGACACGGAGACCAGGCTACATGAGAAGCACAGCGGG
  TTCTGGGATCGGGTTCCTCTCTCCAGCAGTGGGCATGCCACACCCGAGCTCAGCAGGGCTGACAGGCCAG
  CAGTCCCAACACTCACAGTCCAAGGCTGGACAGTGCGGACTTGACCCTGGGAGCCACTGCCAAGCCTCAC
  TGGTGGGCAAGCCTTTTCTCAAGAGCTCCCTTGTCCCTGCTGTGGCCTCTGAGGGCCACCTGCACCCAGC
  CCAGCGCTCTATGAGAAAAAGACCAGTGCACTTTGCGGTTCATTCCAAGAATGACAGCAGACAATCTGAG
  AGGCTGACTGGGTCATTTAAGCCTGGGGACAGTGGGTTTTGGCAAGAATTATTATCTTCAGACAGCTTTA
  AGTCTCTGGCTCCTAGCCTTGATGCACCCTGGAACAAGGGATCAAGGGGCCTGAAGACTGTGAAACCTCT
  GGCATCACCGGCGTTGAATGGCCCCGCTGATATCGCATCCCTTCCCGGCTTCCAAGACACCTTCACTTCC
  AGCTTCAGCTTCATCCAACTCTCCCTTGGTGCTGCTGGAGAACGCGGAGAAGCAGAAGGTTGCCTGCCAT
  CCAGAGAGGCCGAACCTCTGCATCAGAGGCCCCAAGAGATGGCAGCTGAAGCATCTAGCTCAGACAGGCC
  CCACGGGGATCCTCGGCATCTCTGGACCTTCAGTCTTCACGCTGCTCCAGGCTTGGCGGACTTGGCTCAG
  GTGACAAGGAGCAGCAGCAGGCAATCAGAATGTGGCACGGTCTCCTCCTCCTCCTCGGACACTGGCTTCT
  CTTCCCAGGATGCATCCTCCGCTGGTGGGCGGGGCGACCAGGGCGGCGGCTGGGCCGATGCCCATGGATG
  GCATACATTGCTCAGGGAATGGGAGCCCATGCTGCAGGACTACCTACTGAGCAACCGCAGGCAGCTGGAG
  GTCACTTCCTTAATTTTAAAGCTTCAGAAATGTCAAGAAAAAGTGGTCGAGGATGGCGATTACGATACTG
  CAGAGACATTGAGACAGAGGTTGGAAGAACTGGAACAGGAGAAAGGCCGCCTGTCCTGGGCTCTGCCTTC
  ACAGCAACCTGCTCTTCGCAGCTTCTTGGGTTACCTGGCAGCACAGATACAGGTGGCCTTGCATGGAGCC
  ACCCAAAGGGCCGGCAGCGATGATCCAGAAGCCCCACTTGAAGGACAGCTGAGGACTACCGCCCAGGATA
  GCCTGCCTGCATCCATCACCAGGAGGGACTGGCTTATTCGAGAGAAACAGCGATTGCAGAAGGAAATCGA
  AGCTCTCCAAGCACGGATGTCTGCGCTGGAGGCAAAGGAAAAACGGCTGAGCCAAGAGTTGGAGGAGCAG
  GAGGTGCTGCTCCGGTGGCCGGGCTGTGACCTGATGGCACTGGTGGCCCAGATGTCCCCAGGCCAGCTGC
  AGGAGGTCAGCAAGGCCTTGGGAGAGACCCTGACCTCTGCCAACCAGGCTCCCTTCCAGGTGGAGCCACC
  TGAGACCCTCAGGAGCCTCCGGGAAAGGACAAAATCACTGAACCTGGCTGTCAGAGAACTCACTGCTCAG
  GTGTGCTCAGGTGAGAAGCTGTGCAGCTCTCTGAGGAGGAGACTCAGTGACCTCGACACCAGGCTGCCTG
  CCTTGCTGGAAGCCAAGATGCTGGCCCTATCAGAAACACGCATGAAGGCAAACACTGTGAAGTGCATGGA
  AGTGTTGGAAGGTCAGCTGAGCAGCTGCAGGTGCCCGCTGCTTGGGAGAGTGTGGAAAGCAGACTTGGAG
  ACTTGTCAGTTGCTAATGCAGAGCCTGCAGCTTCAGGAAGCAGGCAGCAGCCCACACGCAGAGGACGAGG
  AGCAGGTGCATAGCACAGGAGAGGCCGCCCAGACAGCTGCTCTGGCTGTCCCTCGAACACCCCACCCCGA
  AGAAGAAAAGTCCCCCTTGCAGGTGCTCCAGGAGTGGGACACCCACTCAGCTCTTTCACCACACTGTGCT
  GCAGGCCCATGGAAAGAGGATTCTCACATCGTTTCTGCTGAAGTTGGAGAAAAGTGCGAAGCCATAGGCG
  TGAAGCTCCTACACCTGGAAGACCAGCTTCTCGGAGCCATGTACAGTCACGATGAGGCTCTCTTTCAGTC
  TCTCCAGGGGGAGCTCCAGACGGTGAAGGAAACACTGCAGGCCATGATCCTGCAGCTCCAGCCAACAAAG
  GAGGCAGGAGAGGCCTCAGCTTCCTATCCGACAGCTGGTGCTCAGGAAACCGAGGCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_174853
• Insert Size: 2370 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_174853.2, NP_777278.2
• RefSeq Size: 2408 bp
• RefSeq ORF: 2370 bp
• Locus ID: 244667
• UniProt ID: Q811T9
• Cytogenetics: 8 73.26 cM
• Gene Summary: Involved in the regulation of multiple aspects of embryonic and adult neurogenesis. Required for neural progenitor proliferation in the ventrical/subventrical zone during embryonic brain development and in the adult dentate gyrus of the hippocampus. Participates in the Wnt-mediated neural progenitor proliferation as a positive regulator by modulating GSK3B activity and CTNNB1 abundance. Plays a role as a modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including neuron positioning, dendritic development and synapse formation. Inhibits the activation of AKT-mTOR signaling upon interaction with CCDC88A. Regulates the migration of early-born granule cell precursors toward the dentate gyrus during the hippocampal development. Plays a role, together with PCNT, in the microtubule network formation.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an in-frame segment in the 3' coding region, compared to variant 1. The resulting protein (isoform 2) is shorter when it is compared to isoform 1.