Ufl1 (NM_026194) Mouse Untagged Clone

CAT#: MC221639

Ufl1 (untagged) - Mouse RIKEN cDNA 1810074P20 gene (1810074P20Rik), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ufl1
• Synonyms: 1810074P20Rik; AI429228; Kiaa0776; Maxer; mKIAA0776; Rcad
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221639 representing NM_026194
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGACGCCTGGGAGGAGATCCGGCGGCTGGCGGCTGACTTCCAGCGGGCGCAGTTTGCAGAGTCCA
  CGCAAAGGTTGTCAGAGCGGAACTGCATTGAGATTGTGAATAAATTGATCTCTCAGAAACAGCTGGAAGT
  AGTCCATACACTTGACGGCAAGGAATATATCACTCCAGCCCAAATCAGTAAAGAAATGAGAGACGAGCTA
  CATGTCCGAGGTGGTCGAGTAAACATTGTTGACTTACAACAGGTTATTAATGTGGACCTGACTCATATCG
  AGAGTAGAGTCAGTGATATTATTAAATCAGAGAAGCACGTGCAGATGGTACTGGGACAACTGATTGATGA
  GAACTATCTGGATCAGTTATCAGAAGAGGTGAATGATAAATTGCAAGAAAGTGGTCAAGTAACTGTCTCA
  GAACTGTGTAAAGCCTATGACCTTCCTGGGGATTTTCTGACACAGGCATTAACTCAGCGACTTGGCCGGA
  TTATCAATGGCCATCTGGACCTTGATAACAGAGGAGTGATTTTCACTGAGGCCTTTGTAGCTCGCCATAA
  AGCACGTATCCGCGGACTCTTCAGTGCCATTACACGGCCTACACCAGTGAACTCTTTGGTTTCAAAATAT
  GGATTTCAGGAACAGCTCCTTTACTCTGTGCTCGAGGACCTCGTCAGCACTGGCCGCTTACGAGGCACCG
  TGGTCGGCGGGCGACAGGATAAAGCAGTCTTTGTCCCTGACATCTACTCCAGGACACAGAGTACTTGGGT
  GGACTCCTTTTTCAGGCAGAATGGCTATCTAGAATTTGACGCTTTATCCAGACTTGGAATCCCAGATGCT
  GTGAACTATATAAAGAAAAGGTATAAGAATACCCAACTCTTGTTTTTGAAGGCAACTTGTGTTGGCCAAG
  GACTCGTAGACCAGGTGGAAGCATCCGTAGAGGAAGCCATCAGCTCAGGGACCTGGGTTGACATTTCGCC
  TCTGCTACCCAGTTCCTTGTCAGTTGAAGATGCTGCTATGCTGCTCCAGCAGGTGATGAGACCATTCGGC
  AAGCTCGCTTCAGCTATAGTCTTTAGTGACACGGTTGTGGTGAGTGAAAAGTTCATAACTGACTGCACAG
  GACTGTTCAGCGAACGGATGCACCAAAAAGCTGAGAAGGAAATGAAAAATAATCCTGTGCATTTAATCAC
  TGAAGAAGATCTGAAACAAATTTCCATTTTAGAAAGTGTTAATACAAGTAAAAAGGATAAAAAAGATGAA
  CGCAGGAAGAAAGCAACAGAGGGCTCTGGAAGTGTAAGAGGAGGAGGAGGGGGCAATGCCAGAGAATATA
  AAATTAAGAAAACCAAGAAGAAGGGAAGAAAAGATGAGGACAGTGATGATGAGTCCCAGTCGTCGCATGG
  TGGAAAGAAGAAGCCGGACATCACATTCATGTTCCAGGATGAAATTGAAGATTGCTTAAGGAAACACATT
  CAGGATGCCCCTGAGGAGTTTATTTCAGAGCTTGCAGAATACTTAATAAAGCCTCTTAATAAAATGTATC
  TTGAGGTAGTACGTTCAGTCTTCATGTCTTCAACATCTGCTTCTGGAACTGGCAGAAAACGCACAATCAA
  GGACTTACAAGAAGAGGTTTCAAACCTATACAATAATATCAGATTATTTGAAAAGGGAATGAAATATTTT
  GCAGATGACACACAGACTGCTCTTACCAAGCACTTGCTGAAGACAGTGTGTACCGATATCACTAACCTCA
  TGTTCAACTTCCTAGCCTCAGATTTCCTCATGGCAGTGGAGGAGCCTGCAGCCATTACAAGTGATATAAG
  AAAAAAAATTTTAAGCAAATTGACAGAAGAAACCAAAGTAGCTCTCACAAAACTCCATAACTCTCTCAAT
  GAAAAAAGCATAGAAGACTTCCTGTCTTGCCTGGATTCTGCCACAGAAGCGTGTGACATTATGGTGAAAA
  AGGGAGACAAGAAAAGGGAAAGACAAATCCTCTTCCAGCATCGACAGGCTCTGTGTGAGCAGCTGAAGGT
  CACAGAAGACCCTGCTCTGATTTTACACCTCACAGCAGTCCTGTTATTTCAGCTCTCAACCCACAGCATG
  CTCCACGCACCTGGAAGGTGTGTCCCACAGATCATCGCGTTTCTTCACAGCAAAATCCCAGAGGATCAAC
  ATACTCTCTTGGTAAAATATCAAGGTTTGGTTGTGAAACAGTTAGTCAGTCAAAATAAGAAGACTGGGCA
  GGGAGAGGATCCGTCAAGCGATGAATTAGACAAGGAACAACATGATGTCACAAACGCTACTCGGAAAGAG
  CTTCAGGAACTGTCATTATCCATTAAGGACCTTGTTCTCAAGTCCAGGAAATCATCTGTCACAGAGGAGT
  AA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_026194
• Insert Size: 2382 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_026194.4, NP_080470.2
• RefSeq Size: 4335 bp
• RefSeq ORF: 2382 bp
• Locus ID: 67490
• UniProt ID: Q8CCJ3
• Cytogenetics: 4 A3
• Gene Summary: E3 protein ligase that mediates ufmylation, the covalent attachment of the ubiquitin-like modifier UFM1 to substrate proteins, a post-translational modification on lysine residues of proteins that may play a crucial role in a number of cellular processes. Mediates DDRGK1 ufmylation and may regulate the proteasomal degradation of DDRGK1 and CDK5RAP3 thereby modulating NF-kappa-B signaling. May also play a role in nuclear receptor-mediated transcription through TRIP4 ufmylation. May play a role in the unfolded protein response, mediating the ufmylation of multiple proteins in response to endoplasmic reticulum stress. Anchors CDK5RAP3 in the cytoplasm, preventing its translocation to the nucleus which allows expression of the CCND1 cyclin and progression of cells through the G1/S transition.[UniProtKB/Swiss-Prot Function]