Arhgap10 (NM_030113) Mouse Untagged Clone

CAT#: MC221582

Arhgap10 (untagged) - Mouse Rho GTPase activating protein 10 (Arhgap10), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Arhgap10
• Synonyms: A930033B01Rik; PSGAP-m; PSGAP-s
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221582 representing NM_030113
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCTGCAGCCCCTGGAGTTTAGCGACTGCTACCTCGACAGCCCGTGGTTCCGGGAGAGGATCCGCG
  CGCACGAAGCGGAGCTCGAGAGGACCAACAAGTTCATCAAAGAGCTCATCAAAGACGGCAAGAACCTCAT
  CTCTGCCACCAAAAGTCTGTCAGCTGCCCAGAGGAAGTTCGCTCACTCGCTCAGAGACTTCAAGTTCGAG
  TTTATCGGCGATGCTGAGACTGACGATGAACGGTGCATAGATGCCTCCTTACGGGAATTTTCTAACTTTC
  TGAAGAATCTGGAAGAACAGAGAGAAATCATGGCATTAAGTGTGACTGAAACCCTGATTAAACCCTTGGA
  GAAATTCAGAAAAGAGCAGCTTGGAGCTGTAAAGGAAGAAAAAAAGAAGTTTGACAAAGAGACGGAAAAG
  AATTACAGTCTCATCGACAAGCACTTGACGCTGTCTGCCAGGAAGAAGGACTCACACTTGCAGGAGGCCG
  ACCTCCAAGTGGAGCAGAACCGGCAGCATTTCTACGAGCTCTCGCTTGAATATGTGTGCAAGCTCCAGGA
  AATCCAAGAAAGAAAGAAGTTCGAGTTTGTGGAGCCTATGCTGTCCTTCTTTCAGGGAATGTTCACTTTC
  TATCATCAAGGCCACGAGCTTTCCAAAGACTTCAATCACTACAAGATGGAACTACAAATCAACATTCAGA
  ATACACGGAATCGATTTGAGGGAACCAGATCAGAAGTGGAGGAACTTATGAATAAAATTAGACAAAATCC
  CAAGGACCAGAAGCGAGCCAGCCAGTTCACAGCAGAAGGCTACCTGTACGTCCAGGAGAAAAGGCCTGCT
  CCATTTGGGTCCAGTTGGGTCAAACACTACTGCATGTACCGGAAAACAGCCAAGAAATTCAACATGATTC
  CATTTGAGCACAGATCTGGGGGGAAACTGGGGGATGGCGAGGCTTTCTTTTTGAAAGAATGCACCAAAAG
  GCACATGGACTCCACGGACAGAAGGTTTTGCTTTGACATCGAAGCTGCTGACAGGCCTGGCGTCCCCTTG
  ACCGTGCAGGCGTTTTCAGAAGAAGAGAGGAAGCAGTGGTTGGAAGCTCTGGGTGGAAAAGAAGCTCTGT
  TCCACACGTTTAACAGAGCCATTGTCCCGAGACCGGAAGGAGGTGCACAGCTGGATAAGATGGGATTCAC
  AATTCTCAGAAAATGCATCAGCGCCGTTGAAACCCGAGGTATAAATGACCAAGGACTGTACAGAGTTGTG
  GGGGTGAGTTCAAAGGTCCAGAGACTTCTGAGTATGTTGATGGATGTAAAAATGTGCAATGAGCTGGACC
  TGGAGAATTCTGCAGATTGGGAAGTGAAGACAGTAACAAGTGCCTTGAAGCAGTATCTGAGGAGTCTTCC
  AGAGCCTCTCATGACCTATGAGTTACACAGAGACTTCATTGTTCCAGCCAAGAGCGGCAGCCCGGAATCC
  CGAGTTAATGCCATCCACTTCTTGGTACACAAACTGCCAGAGAAGAATAAGGAGATGTTGGATATCTTGG
  TGAAGCATCTAACCAACGTGTCAAGTCACTCGAAGCAGAATCTAATGACAGTGGCGAATTTAGGGGTGGT
  GTTTGGACCAACTCTGATGAGACCACAGGAAGAGACTGTTGCCGCCATCATGGATTTGAAGTTTCAGAAT
  ATCGTTGTAGAAATCTTAATTGAAAACCATGAAAAGATTTTTCGAACCTCGCCTGATACCACATTTGCTG
  AGCCCACATGCCTGTCAGCATCACCCCCAAACGCTCCACCACGACAGTCCAAGAGACAAGGGCAGAGGAC
  GAAGAGACCAGTGGCCGTGTACAACCTTTGTCTTGAGCTGGAGGAGGGTGACAGTCCCAGCCCTCTCAAG
  GAGGACCCCCCCAGCAGCAGTCAGGACTCTCTCTCCACCCCATCTCCCACAACGTCAGCTGCCCATGGGC
  CTCCTGGACTAGACGGAAACCACCTAGCAGCAGACGGAGGGAGCTGTGGAGATGCCACCGCCACCACGCC
  AAGCCAGACCCGGCCATCCATGGTGCAGTGGCTAAATATGCAGTCTCCAACCACACCAAGCTCCAACCCA
  GCTGGAACACCTCCTTCACCCAGAATGTCACCGTTCCCCCTCTCTCCTGCTGCCTCCATTGTAGACAAGC
  TGCCTGAGTGCGTTATCAATCGAAAGGCTCGGGCAGTGTACCCGTGTGAAGCAGAACACAGCTCGGAATT
  GTCATTTGAAATAGGAGCCATTTTTGAGGATGTTCAGACCTCAAGGGAACCCGGATGGCTGGAAGGGACC
  CTGAACGGCAAGCGAGGGCTGATTCCACAGAATTATGTCAAGCTCCTGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_030113
• Insert Size: 2361 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030113.2, NP_084389.2
• RefSeq Size: 3038 bp
• RefSeq ORF: 2361 bp
• Locus ID: 78514
• UniProt ID: Q6Y5D8
• Cytogenetics: 8 C1
• Gene Summary: GTPase activator for the small GTPases RhoA and Cdc42 by converting them to an inactive GDP-bound state. Essential for PTKB2 regulation of cytoskeletal organization via Rho family GTPases. Inhibits PAK2 proteolytic fragment PAK-2p34 kinase activity and changes its localization from the nucleus to the perinuclear region. Stabilizes PAK-2p34 thereby increasing stimulation of cell death.[UniProtKB/Swiss-Prot Function]