Vps51 (NM_001081041) Mouse Untagged Clone

CAT#: MC221574

Vps51 (untagged) - Mouse RIKEN cDNA 1110014N23 gene (1110014N23Rik), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Vps51
• Synonyms: 1110014N23Rik; 3110057M17Rik; AI837850; Ffr
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221574 representing NM_001081041
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCTGCGGCAGCTGTGGGGCCTGGCCTAGGGTCCGGGCCTGGAGACTCCCCAGAAGGTCCCGAGG
  CGGACGCTCCGGAGCGTCGGCGGAAGGCTCACGGGATGCTGAAGCTTTACTACGGCCTCTCGGAGGGGGA
  GGTGGCGGGGCATCCCGCAGGGCCTGACCCTCTGGACCCAACCGATCTCAATGGGGCGCACTTCGACCCG
  GAAGTTTATCTTGACAAGCTGCGTAGAGAATGTCCTCTGGCCCAGCTGATGGACAGTGAGACGGACATGG
  TACGGCAGATCCGGGCTCTAGACAGCGACATGCAGACCCTGGTCTATGAGAACTACAACAAGTTCATCTC
  AGCTACAGACACCATTCGAAAGATGAAGAATGACTTCCGGAAGATGGAAGATGAGATGGACCGGCTGGCC
  ACCAACATGGCAGTGATCACCAACTTCAGCGCACGGATCAGCGCCACGCTACAGGACCGACACGAGCGCA
  TCACCAAGCTAGCAGGGGTACATGCACTACTGCGGAAGCTGCAGTTCCTTTTTGAGCTGCCTTCGCGCCT
  CACCAAGTGTGTGGAGCTGGGTGCCTATGGGCAGGCAGTGCGCTATCAGGGTCGTGCCAGGGCCGTGCTG
  CAGCAGTACCAGCACCTGCCCTCCTTCCGTGCCATACAGGATGACTGCCAGGTCATCACAGCTCGCTTGG
  CCCAGCAGCTGCGGCAGCGCTTCAGGGAAGGCTGCTCAGGTGCCCCGGAGCAGGCCGAGTGTGTGGAGCT
  GCTGCTGGCCCTGGGCGAGCCAGCTGAGGAGCTGTGCGAGGAGTTCCTGGCGCATGCGCGGGGACGGCTG
  GAGGAAGAACTGAGCAGCCTGGAGGCCGAACTGGGGCCCTCCCCTCCAGCACCTGATGTGTTAGAGTTCA
  CCGACCGTGGTGGCAATGGTTTTGTAGGTGGCCTCTGCCAGGTGGCAGCAGCCTACCAAGAGCTGTTTGC
  TGCTCAGGGCCCTGCTGGTGCGGAGAAGCTGGCAGCCTTTGCGCAGGAGCTGGGCGGCCGCTACTTTGCA
  CTGGTTGAGCGGCGGCTGGCACAGGAGCAGGGTGGTAGTGACAACTCACTGCTGGTGCGGGCACTAGATC
  GCTTCCACCGGCGCCTGCGAGCACCTGGGGCCCTGCTGGCCGCTGCTGGGCTTTCAGAGTCAGCCACAGA
  GATTGTGGAGCGAGTGGCCCGGGAGCGCCTGAGCCACCATCTGCAGGGTCTGAAGGCTGCCTTCCTGAGC
  TCCCTGACGGATGTTCGCCAGGCCCTGGCAGCACCTCGCCTAGCGGGGAAGGAGGGCCCCAGCCTGGCTG
  AGTTGCTGGCCAATGTGGCCAGCTCCATCCTAAGCCACATCAAGACGTCACTGGCTTCTGTGCATCTCTT
  TACCGCCAAGGAGGTGTCCTTCTCCAATAAGCCCTACTTCAGGGGCGAGTTCTGCAGCCAGGGAGTTCGT
  GAGGGCCTCATCGTGGGTTTCATCCGCTCCATGTGCCAGACAGCCCAGAGCTTCTGTGACAGCCCTGGAG
  AAAAGGGAGGTGCCACGCCGCCTGCCCTGCTCCTGCTGCTCTCTCGCCTCTGCCTGGATTATGAGACAGC
  CACCATCTCCTACATCCTCACACTCACTGATGAACAGTTTCTGGTGCAGGACCAGTCTCCCGTTACACCC
  GTGAGCACACTGTGTGCAGAGGCCAGAGAGACAGCACGGCGGCTGCTGACTCACTATGTGAAGGTGCAAG
  GCCTGGTCATATCCCAGATGCTACGTAAGAGTGTGGAGACCCGGGACTGGCTCAGTACTCTGGAGCCTAG
  GAACGTGCGTGCTGTCATGAAGCGTGTGGTGGAGGACACTACAGCTATTGATGTCCAGGTGGGGCTCCTG
  TATGAAGAGGGTGTCCGGAAGGCCCAGAGCAGTGACTCAAGCAAAAGGACTTTCTCTGTGTACAGCAGCT
  CCCGGCAGCAGGGCCGCTATGCACCCAGCTACACACCCAGTGCTCCCATGGACACAAACCTCCTGAGCAA
  TATTCAGAAGCTGTTCTCTGAACGGATCGATGTGTTCAGCCCCGTGGAATTCAACAAGGTGTCGGTGCTG
  ACTGGCATCATTAAGATCAGCTTGAAGACACTACTGGAGTGTGTGCGCCTACGCACCTTTGGGCGCTTTG
  GGCTGCAGCAGGTGCAGGTGGACTGCCACTTCCTGCAGCTTTACTTGTGGCGCTTTGTGGCTGATGAGGA
  GCTAGTGCACCTGCTGCTGGACGAAGTAGTAGCCTCAGCTGCTCTGCGCTGTCCCGACCCAGTGCCCATG
  GAACCTAGTGTTGTCGAGGTCATCTGCGAGCGCGGCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001081041
• Insert Size: 2349 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081041.1, NP_001074510.1
• RefSeq Size: 2631 bp
• RefSeq ORF: 2349 bp
• Locus ID: 68505
• UniProt ID: Q3UVL4
• Cytogenetics: 19 A
• Gene Summary: Acts as component of the GARP complex that is involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN). The GARP complex is required for the maintenance of protein retrieval from endosomes to the TGN, acid hydrolase sorting, lysosome function, endosomal cholesterol traffic and autophagy. VPS51 participates in retrograde transport of acid hydrolase receptors, likely by promoting tethering and SNARE-dependent fusion of endosome-derived carriers to the TGN. Acts as component of the EARP complex that is involved in endocytic recycling. The EARP complex associates with Rab4-positive endosomes and promotes recycling of internalized transferrin receptor (TFRC) to the plasma membrane.[UniProtKB/Swiss-Prot Function]