Tle3 (NM_001083927) Mouse Untagged Clone

CAT#: MC221568

Tle3 (untagged) - Mouse transducin-like enhancer of split 3, homolog of Drosophila E(spl) (Tle3), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tle3
• Synonyms: 2610103N05Rik; ESG; Grg3a; Grg3b; mKIAA1547
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221568 representing NM_001083927
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTATCCGCAAGGCAGACATCCGGCACCCCATCAACCCGGGCAGCCGGGATTTAAATTCACTGTGGCCG
  AGTCCTGTGACAGGATCAAAGACGAATTCCAGTTCCTGCAAGCTCAGTATCACAGCCTCAAAGTGGAGTA
  TGACAAGCTGGCTAACGAGAAGACGGAGATGCAGCGCCATTATGTGATGTACTATGAGATGTCCTATGGC
  TTGAATATTGAAATGCACAAGCAGACAGAGATTGCGAAGAGACTGAACACAATCCTAGCCCAGATCATGC
  CTTTTTTGTCACAGGAGCATCAGCAGCAAGTGGCGCAGGCTGTGGAACGCGCCAAGCAGGTCACCATGAC
  GGAGTTGAACGCCATCATCGGGGTACGTGGACTCCCCAATCTGCCTCTCACCCAGCAGCAACTCCAGGCC
  CAGCACCTCTCCCATGCCACGCATGGTCCCCCGGTCCAGCTGCCACCCCACCCGTCAGGCCTCCAGCCTC
  CTGGGATTCCCCCAGTGACAGGAAGCAGCTCTGGGTTGCTGGCACTTGGTGCCCTGGGAAGTCAAGCTCA
  CTTGGCGGTGAAGGATGAGAAGAACCACCATGAACTGGATCACAGAGAGAGAGAGTCCAGCACGAACAAT
  TCCGTGTCACCCTCTGAAAGCCTCCGGGCCAGTGAGAAGCACCGGGGCTCTGCAGACTACAGCATGGAAG
  CCAAGAAGCGGAAGGCGGAAGAGAAAGACAGCCTCAGCAGATACGATAGCGATGGGGACAAGAGTGACGA
  CCTGGTGGTGGATGTCTCTAATGAGGACCCAGCAACACCCCGGGTGAGCCCAGCACACTCCCCTCCTGAA
  AATGGGCTGGACAAAGCCCGTGGTCTGAAGAAAGATGCCCCCACCAGCCCAGCCTCCGTGGCTTCCTCCA
  GCAGCACACCTTCCTCCAAGACCAAAGACCTTGGTCATAATGACAAATCTTCCACACCTGGGCTCAAGTC
  CAACACACCAACGCCAAGAAATGATGCCCCAACTCCAGGCACCAGCACCACCCCGGGGCTCCGGTCAATG
  CCGGGCAAACCTCCAGGCATGGACCCGATAGGTATAATGGCCTCGGCCCTGCGAACACCCATCACCCTCA
  CCAGCTCCTATCCAGCACCCTTTGCCATGATGAGCCACCACGAGATGAATGGCTCCCTCACCAGCCCAAG
  CGCCTATGCTGGCCTACACAACATCCCATCCCAGATGAGCGCCGCCGCAGCCGCTGCAGCCGCCGCCTAT
  GGCCGATCGCCAATGGTGAGCTTTGGAGCTGTTGGTTTTGACCCTCACCCCCCAATGAGGGCCACAGGCC
  TGCCTTCCAGTCTCGCCTCCATTCCTGGAGGGAAACCGGCATACTCCTTCCATGTGAGTGCTGATGGGCA
  GATGCAACCTGTGCCCTTCCCCCATGATGCACTAGCAGGCCCTGGCATTCCCAGGCATGCCCGGCAGATC
  AATACGCTCAGCCATGGAGAGGTGGTATGTGCTGTGACCATCAGCAACCCCACACGACACGTCTACACAG
  GCGGCAAGGGCTGTGTGAAGATATGGGACATCAGCCAGCCGGGCAGCAAGAGTCCCATCTCCCAGCTGGA
  CTGCCTGAACAGGGACAACTACATCCGCTCGTGCAAGCTTCTCCCCGATGGGCGCACGCTCATTGTGGGT
  GGTGAGGCCAGCACGCTCACCATCTGGGACCTGGCCTCACCCACACCCCGCATCAAGGCTGAGCTGACGT
  CCTCGGCTCCAGCCTGTTATGCCCTGGCCATCAGTCCTGATGCCAAAGTCTGTTTTTCCTGCTGCAGCGA
  CGGGAACATTGCGGTTTGGGATCTGCACAACCAGACCCTGGTCAGGCAGTTCCAGGGCCACACAGATGGG
  GCCAGCTGTATAGACATCTCTCATGATGGCACTAAGCTGTGGACCGGGGGCCTGGACAACACCGTGCGCT
  CCTGGGACCTACGTGAAGGACGGCAGTTACAGCAACACGATTTCACCTCCCAGATCTTCTCCCTGGGTTA
  CTGCCCCACTGGGGAGTGGCTGGCCGTGGGCATGGAGAGCAGCAATGTGGAGGTCCTGCACCACACTAAG
  CCCGACAAATACCAGCTGCACCTGCACGAGAGCTGCGTGCTGTCCCTCAAGTTCGCCTATTGTGGCAAGT
  GGTTTGTGAGCACTGGGAAAGACAACCTTCTCAATGCCTGGAGGACGCCTTATGGAGCCAGCATCTTCCA
  GTCAAAAGAATCCTCATCTGTCTTGAGCTGTGACATTTCAGCGGATGACAAATATATTGTAACAGGCTCT
  GGTGACAAGAAGGCCACAGTTTACGAGGTCATCTACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001083927
• Insert Size: 2349 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001083927.1, NP_001077396.1
• RefSeq Size: 5202 bp
• RefSeq ORF: 2349 bp
• Locus ID: 21887
• UniProt ID: Q08122
• Cytogenetics: 9 B
• Gene Summary: Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES (By similarity). May play an important role during spermatogenesis.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest protein (isoform 1).