Stat5b (NM_011489) Mouse Untagged Clone

CAT#: MC221567

Stat5b (untagged) - Mouse signal transducer and activator of transcription 5B (Stat5b), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Stat5b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221567 representing NM_011489
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTATGTGGATACAGGCTCAGCAGCTCCAGGGCGATGCCCTTCACCAGATGCAGGCCTTGTACGGCC
  AGCATTTCCCCATCGAGGTGCGACATTATTTATCACAGTGGATCGAAAGCCAAGCCTGGGACTCAATAGA
  TCTTGATAATCCACAGGAGAACATTAAGGCCACCCAGCTCCTGGAGGGCCTGGTGCAGGAGCTGCAGAAG
  AAGGCGGAGCACCAGGTGGGGGAAGATGGGTTTTTGCTGAAGATCAAGCTGGGGCACTATGCCACACAGC
  TCCAGAGCACGTACGACCGCTGCCCCATGGAGCTGGTTCGCTGTATCCGGCACATTCTGTACAACGAACA
  GAGGCTGGTTCGCGAAGCCAACAACGGCAGCTCTCCAGCTGGAAGTCTTGCTGACGCCATGTCCCAGAAG
  CACCTTCAGATCAACCAAACGTTTGAGGAGCTGCGCCTGATCACACAGGACACGGAGAACGAGCTGAAGA
  AGCTGCAGCAGACCCAAGAGTACTTCATCATCCAGTACCAGGAGAGCCTGCGGATCCAAGCTCAGTTTGC
  CCAGCTGGGACAGCTGAACCCCCAGGAGCGCATGAGCAGGGAGACGGCCCTCCAGCAGAAGCAAGTGTCC
  CTGGAGACCTGGCTGCAGCGAGAGGCACAGACACTGCAGCAGTACCGAGTGGAGCTGGCTGAGAAGCACC
  AGAAGACCCTGCAGCTGCTGCGGAAGCAGCAGACCATCATCCTGGACGACGAGCTGATCCAGTGGAAGCG
  GAGACAGCAGCTGGCCGGGAACGGGGGTCCCCCCGAGGGCAGCCTGGACGTGCTGCAGTCCTGGTGTGAG
  AAGCTGGCCGAGATCATCTGGCAGAACCGGCAGCAGATCCGCAGGGCTGAGCACTTGTGCCAGCAGCTGC
  CCATCCCAGGCCCCGTGGAGGAGATGCTGGCTGAGGTCAACGCCACCATCACGGACATCATCTCAGCCCT
  GGTCACCAGCACGTTCATCATCGAGAAGCAGCCTCCTCAGGTCCTGAAGACCCAGACCAAGTTTGCAGCC
  ACCGTGCGCCTGCTGGTGGGGGGGAAGCTGAATGTGCACATGAACCCCCCGCAGGTGAAGGCGACCATCA
  TCAGCGAGCAGCAGGCCAAGTCCCTGCTCAAGAATGAGAACACCCGCAATGATTACAGCGGCGAGATCCT
  GAACAACTGTTGCGTCATGGAGTACCACCAGGCCACTGGCACACTCAGCGCCCACTTCAGAAACATGTCC
  CTGAAACGAATCAAGAGGTCTGACCGCCGTGGGGCAGAGTCAGTAACGGAAGAGAAGTTCACGATCCTGT
  TTGACTCACAGTTCAGCGTCGGTGGAAACGAGCTGGTCTTTCAAGTCAAGACCTTGTCGCTCCCGGTGGT
  GGTGATTGTTCACGGCAGCCAGGACAACAATGCCACAGCCACTGTCCTCTGGGACAACGCCTTTGCAGAG
  CCTGGCAGGGTGCCATTTGCCGTGCCTGACAAGGTGCTGTGGCCGCAGCTGTGTGAAGCGCTCAACATGA
  AATTCAAGGCTGAAGTACAGAGCAACCGGGGCTTGACCAAGGAGAACCTCGTGTTCCTGGCACAGAAACT
  GTTCAACATCAGCAGCAACCACCTCGAGGACTACAACAGCATGTCCGTGTCCTGGTCCCAGTTCAACCGG
  GAGAATTTGCCAGGACGGAATTACACTTTCTGGCAGTGGTTTGATGGCGTGATGGAAGTATTGAAAAAAC
  ATCTCAAGCCTCACTGGAATGATGGGGCTATCCTGGGTTTCGTGAACAAGCAACAGGCCCACGACCTGCT
  CATCAACAAGCCAGACGGGACCTTCCTGCTGCGCTTCAGCGACTCGGAAATCGGGGGCATCACCATTGCT
  TGGAAGTTTGACTCTCAGGAGAGAATGTTTTGGAATCTGATGCCTTTTACCACTAGAGACTTCTCTATCC
  GGTCCCTCGCTGACCGCCTGGGGGACCTGAATTACCTCATATATGTGTTTCCTGATCGGCCAAAGGATGA
  AGTATATTCTAAGTACTACACACCGGTCCCCTGTGAGCCCGCAACTGCGAAAGCAGCTGACGGATACGTG
  AAGCCACAGATCAAGCAGGTGGTCCCCGAGTTTGCAAATGCATCCACAGATGCTGGGAGTGGCGCCACCT
  ACATGGATCAGGCTCCTTCCCCAGTCGTGTGCCCTCAGGCTCACTACAACATGTACCCACCCAACCCGGA
  CTCCGTCCTTGATACCGATGGGGACTTCGATCTGGAAGACACGATGGACGTGGCGCGGCGCGTGGAAGAG
  CTCTTAGGCCGGCCCATGGACAGTCAGTGGATCCCTCACGCACAGTCATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_011489
• Insert Size: 2361 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011489.3, NP_035619.3
• RefSeq Size: 5255 bp
• RefSeq ORF: 2361 bp
• Locus ID: 20851
• UniProt ID: P42232
• Cytogenetics: 11 63.63 cM
• Gene Summary: Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Binds to the GAS element and activates PRL-induced transcription. Positively regulates hematopoietic/erythroid differentiation.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same isoform. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.