Cd44 (NM_009851) Mouse Untagged Clone

CAT#: MC221553

Cd44 (untagged) - Mouse CD44 antigen (Cd44), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cd44
• Synonyms: AU023126; AW121933; AW146109; HERMES; Ly-24; Pgp-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NM_009851.2
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACAAGTTTTGGTGGCACACAGCTTGGGGACTTTGCCTCTTGCAGTTGAGCCTGGCACATCCACATC
  AGCAGATCGATTTGAATGTAACCTGCCGCTACGCAGGTGTATTCCATGTGGAGAAAAATGGCCGCTACAG
  TATCTCCCGGACTGAGGCAGCTGACCTCTGCCAGGCTTTCAACAGTACCTTACCCACCATGGACCAAATG
  AAGTTGGCCCTGAGCAAGGGTTTTGAAACATGCAGGTATGGGTTCATAGAAGGAAATGTGGTAATTCCGA
  GGATTCATCCCAACGCTATCTGTGCAGCCAACCACACAGGAGTATATATCCTCGTCACGTCCAACACCTC
  CCACTATGACACATATTGCTTCAATGCCTCAGCCCCTCCTGAAGAAGACTGTACATCAGTCACAGACCTA
  CCCAATTCCTTCGATGGACCGGTTACCATAACTATTGTCAACCGTGATGGTACTCGCTACAGCAAGAAGG
  GCGAGTATAGAACACACCAAGAAGACATCGATGCTTCAAACATTATAGATGACGATGTCAGCAGCGGCTC
  CACCATCGAGAAGAGCACCCCAGAAAGCTACATTTTGCACACCTACCTTCCTACTGAACAGCCTACTGGA
  GATCAGGATGACTCCTTCTTTATCCGGAGCACCTTGGCCACCATTGCCTCAACTGTGCACTCAAAAAGCC
  ATGCAGCAGCTCAGAAACAAAATAATTGGATATGGTCTTGGTTTGGTAATTCACAGTCCACAACTCAGAC
  TCAGGAGCCCACAACGAGTGCAACTACAGCCTTGATGACCACCCCTGAAACACCACCCAAGAGGCAAGAA
  GCCCAGAACTGGTTTTCATGGTTATTTCAACCATCAGAGTCCAAGAGTCATCTTCATACAACAACGAAGA
  TGCCTGGTACGGAGTCAAATACCAACCCAACAGGCTGGGAGCCAAATGAGGAAAATGAAGATGAAACAGA
  CAAATACCCCAGTTTTTCTGGATCAGGCATTGATGATGATGAAGATTTTATCTCCAGTACCATTGCAAGT
  ACTCCACGGGTTTCTGCCCGCACAGAAGACAACCAGGACTGGACCCAGTGGAAGCCAAACCATTCAAACC
  CAGAAGTACTACTCCAGACAACCACCAGGATGGCTGATATAGACAGAATCAGCACCAGTGCTCATGGAGA
  AAATTGGACCCCGGAACCACAGCCTCCTTTCAATAACCATGAGTATCAGGATGAAGAGGAGACCCCACAT
  GCTACAAGCACAACTCCTAATAGTACAGCAGAAGCAGCAGCTACCCAGCAGGAGACGTGGTTTCAGAACG
  GATGGCAGGGGAAGAACCCCCCTACCCCAAGTGAAGACTCCCATGTGACAGAAGGGACAACTGCTTCGGC
  CCACAACAACCATCCAAGTCAAAGAATAACAACACAGAGTCAAGAGGATGTTTCCTGGACAGATTTCTTC
  GACCCAATCTCACATCCAATGGGACAAGGTCATCAAACAGAAAGCAAGGATACAGACTCCAGTCATAGTA
  CAACCCTTCAGCCTACTGCAGCTCCAAATACCCATTTGGTGGAAGACTTGAACAGGACAGGACCACTTTC
  AGTGACAACTCCACAGAGTCATTCTCAGAACTTCTCTACATTACATGGAGAGCCGGAAGAAGACGAAAAC
  CATCCAACAACTTCTATTCTGCCATCTAGCACTAAGAGCGGCGCTAAAGATGCAAGAAGAGGTGGAAGTC
  TTCCCACAGATACAACTACTTCTGTAGAAGGCTACACCTTTCAATACCCAGACACAATGGAAAATGGGAC
  TCTCTTCCCAGTGACCCCTGCTAAAACTGAGGTCTTTGGAGAAACTGAAGTGACTCTTGCTACTGACTCC
  AACGTTAATGTTGATGGCTCCTTACCAGGAGATCGAGACTCATCCAAGGACTCCAGGGGGAGTTCCCGCA
  CTGTGACTCATGGATCCGAATTAGCTGGACACTCAAGTGCGAACCAGGACAGTGGAGTGACCACAACTTC
  TGGTCCTATGAGGAGACCTCAGATTCCAGAATGGCTCATCATCTTGGCATCTCTCCTGGCACTGGCTCTG
  ATTCTTGCCGTCTGCATCGCGGTCAATAGTAGGAGAAGGTGTGGGCAGAAGAAAAAGCTGGTGATCAACG
  GTGGCAATGGGACGGTGGAAGACAGGAAACCCAGTGAGCTCAACGGGGAGGCCAGCAAGTCTCAGGAAAT
  GGTGCATTTGGTGAACAAGGAACCATCAGAGACCCCGGACCAGTGTATGACAGCTGACGAGACCCGGAAT
  CTGCAGAGTGTGGACATGAAGATTGGGGTGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009851
• Insert Size: 2343 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009851.2, NP_033981.2
• RefSeq Size: 5654 bp
• RefSeq ORF: 2343 bp
• Locus ID: 12505
• UniProt ID: P15379
• Cytogenetics: 2 54.13 cM
• Gene Summary: Cell-surface receptor that plays a role in cell-cell interactions, cell adhesion and migration, helping them to sense and respond to changes in the tissue microenvironment. Participates thereby in a wide variety of cellular functions including the activation, recirculation and homing of T-lymphocytes, hematopoiesis, inflammation and response to bacterial infection. Engages, through its ectodomain, extracellular matrix components such as hyaluronan/HA, collagen, growth factors, cytokines or proteases and serves as a platform for signal transduction by assembling, via its cytoplasmic domain, protein complexes containing receptor kinases and membrane proteases (PubMed:8343954, PubMed:25065622). Such effectors include PKN2, the RhoGTPases RAC1 and RHOA, Rho-kinases and phospholipase C that coordinate signaling pathways promoting calcium mobilization and actin-mediated cytoskeleton reorganization essential for cell migration and adhesion (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and it encodes the longest protein (isoform a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.