Rfwd3 (NM_146218) Mouse Untagged Clone

CAT#: MC221529

Rfwd3 (untagged) - Mouse ring finger and WD repeat domain 3 (Rfwd3), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rfwd3
• Synonyms: BC027246
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221529 representing NM_146218
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGAAGTCGCGGTCGGCCAGGCGTCCGACTTGGTCCCCTCAGAGATGGATCATGAAGTGATATACA
  GTCATCTTCAGGGACCATTAGAGGGTACTATAGAACCAGCTACTCCAACTGAAGTCGTCAGCAATGGGGC
  ACCTCTCCAGCCTGCCCCTGCTGAACTGGCCAATAGCCAAGGTGGAGCACATGTTCAGCCTGCCCCTGCT
  GAAGTGGTCAGCAGCCAAGATGGACTGCCCACTCTACAGCCTCTGCCACCAGCATCCATTGATTTGACGG
  AGGAAGTACAGCCCTCAGAAGAAAATATGGAGGTTGTCAATCCTGGAACTTCAGAGGAGCCTAGTCAGGG
  ATCTGGTGCTAACCCAACCGCCGGAGCTGCTAGATCCGTTTCAATGAACAACTTCATCAGCGGGCTGCAG
  AGGCTTCATAACATGCTGGAATTGCTGAGACCTCCACCTGCAGACCACAGTGTGGGGCCAATAAGAACAA
  GGAGGAGGATGGCACCCATTTTGAGGGCCAGAGCTGGAGAGTCTCAGAGGCAAGACAATGGCAGGTATGT
  GCCACATACACCACTATATGCCTACTTTCAGGTAAGCAGGTCTCAGCCTTACCCACTACCCGCTGCTCAT
  GATTCAGAGACCAGGAATCCACCGTCAGGCACTGACTCTGACAGCGATGGCTCTGCAGAGGATGAAGAGG
  TTGTTGTCCAGGCAGAAGAACCTGAAGCCAACATTCCAGAACAAGGAGTTATCGCAACAGACCAAGAAGC
  TACATCTGTCACAGGAGATGATGCAGTCCCTAAAGAGTCTCCCCAGAAGCCCAACATGCTATCTGCTATG
  GAAGATGAGGAAGGTGAAACTTGCACAATATGTTTGGAACAATGGACCAATGCTGGGGACCACCGGATCT
  CAGCGCTACGCTGTGGGCACCTCTTTGGATTTAGATGCATTTCTAAATGGCTCAAAGGACAAACACGAAA
  ATGTCCTCAGTGCAACAAGAAAGCCAAGCACAGTGACATCGTTGTCATTTATGCCCGATCCCTGCGAGCT
  TTGGACACTAGTGAACAGGAGCGCATGAAAAGTGACTTACTGAATGAGCAAATGCTCAGGAAGCAAGCTG
  AGTTAGAGTCTGCACAGTGCCGGCTCCAGCTTCAGGTCCTTATTGATAAGTGCACTAAACTCAATAGCCG
  TGTCCAGGACTTGGAAAAGTTTGTTGTGCGGCATCAGAACTCAGCTGCGCAGCAGTGCAGTCGCTCGAAA
  GCACTTTGTGGGAAATGCCTGTCCTCCAGCCAGAGCCCAGGCAAATACAGCTCAGAAAAGACCTTCACCA
  TATCTCAGACGGGAAAATGCCGAATCTTGGCCTATTGTGATGGTCTGAGCTGCCTGGTGGCCTCCCAGCC
  CTCCCCTCAGGCCTCCTTTCTTCCAGGCTTTGGTGTTAAGATGTTGAGTACTGCCAACATGAAGAGCAGT
  CAGTATATTCCTATGCACAACAAGCAGATTCGTGGATTATCTTTCAGCAGTCAGTCCAAAGGCTTGCTCC
  TGTCTGCCTCCCTTGACTGCACTATTAAGCTGACCAGCCTAGAAACAAATACTGTGGTGCAGACTTACAA
  CACTGGGCGTCCTGTCTGGAGCTGCTGCTGGTGTCTTGACGAAAACAATTACGTCTATGCTGGACTAGCC
  AGTGGTTCAATTCTGATCTATGACTTACGGAACACAAACACCTATATTCAGGAATTAGTTCCTCAGAAAG
  CCAGATGTCCTCTGGTATCCCTGTCATATTTACCCAAAGCTGCAGTGGCTGCATTTCCTTATGGTGGGGT
  GCTGGCTGGAACATTGGAGAATGCCTCCTTCTGGGAGCTAAAATCAGACTTTTCTCATAAGCCTCATGTG
  CTGTCCTTGGAGCCAGGGGGCTTTGTAGACTTTCAGACAGAGAGCAGCACCCGACACTGTCTTGTGACTT
  ACAGACCTGATAAAAGCCATAACACTGTCCGAACTGTGCTATTGGAGATGTCTTACAAACTGGACGATGC
  TGGAGAGCCGGTCTGTTCCTGTCTTCCTGTACAAACATTCCTTGGGGGACCCACTTGCAAACTGTTGACC
  AAAAGTGCCATCTTCCAAAGCCCAGAGAATAATGGCAATGTCCTAGTATGTACTGGGGATGAAGCGTCAC
  AGTCTACACTGTTGTGGAATGCTGGCAGTGGCTTGTTGCTGCAGAACCTGAAGGCTGGTGAACCTGTCCT
  GGACATCTGCCCATTTGAAACGAATCAGAACAGCTATCTGGCCACCTTGACAGAGAAGACTGTCCATATG
  TACAAGTGGGAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_146218
• Insert Size: 2325 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_146218.4, NP_666330.2
• RefSeq Size: 4443 bp
• RefSeq ORF: 2325 bp
• Locus ID: 234736
• UniProt ID: Q8CIK8
• Cytogenetics: 8 E1
• Gene Summary: E3 ubiquitin-protein ligase required for the repair of DNA interstrand cross-links (ICL) in response to DNA damage. Plays a key role in RPA-mediated DNA damage signaling and repair. Acts by mediating ubiquitination of the RPA complex (RPA1, RPA2 and RPA3 subunits) and RAD51 at stalled replication forks, leading to remove them from DNA damage sites and promote homologous recombination. Also mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint. May act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome. In response to ionizing radiation, interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53/TP53.[UniProtKB/Swiss-Prot Function]