Jhy (NM_001081121) Mouse Untagged Clone

CAT#: MC221504

4931429I11Rik (untagged) - Mouse RIKEN cDNA 4931429I11 gene (4931429I11Rik), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Jhy
• Synonyms: 4931429I11Rik; BB239335
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221504 representing NM_001081121
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATAAATATAAACCCGTTCCCAAGCTCTCTAGCCAGTCTCCCGTCCATCACACCAACTTGAGGATCC
  CGCCTTCGGGCAGACCCTTTAAGAAAGAAGACTTCTTAAACCTGATTTCAAAAGACTCCTTAGAATCGGA
  TTCAGAAAGCCTCCCTCAAGAGGCTAAGTCCTGGTCTGATATTAAGGACCAAATCCAGGACAAAGATATG
  GAGCCTGACAGCTTGGAGGAGGACAGCCCAAGTGAGACAGAAGAGGCAGTGAACAGAAAAGCAGCCCACA
  ACACTAACAGGGAAGACCTAAGTGCTCATGATGCTGGTGTGAATCACAGCCAACATCAGGTAGAAGACAA
  ATACTCAGACCTCCGATATGATCCCAACTGGAAGAACAAAAGAGAGGAGGGGCAGCCATTGGCTGGAGAA
  GCATTACCAGGGTCTGCAGATAGCTCTTCAGAAAACCTGCCTTTGGCTCCGCTCTACCCTTCCAGAGAGC
  CATCCATGGGACTCTCCGCTGGGAAAGGCAAAGAAAAGAAGAGTCCTCAGAGCGAAGCCTCCTTACTTGG
  AAGCGAATTTCTGAGCCCAAAGTATGAACGTAGCACCCGTCAAAATGGGTTCTTCTCAGAACTAAGCGAC
  AGTGACCAGGAGGAGAAATCCAGCGGCCTGTCTCAGTACCTAAAGAGCTCAAGCTCACACAACGACGTTT
  TCCTGCCGGGGTCACGTGGCCCGCGCAGAAGGAAGTCCAAACAATATTTTGTGGAAAAAAACAAGCTGAC
  TTTGGGGTTACCTATGCCTAAAACAGACTCTTACCTTCAACTTCACAATAAGAAGAGAGGAGAGACCCGC
  CTCGAACAGATTTCCTACCCCGTCAGAGATACTGACAAGATGACTGTCCAGAATGACAAAGAAGTCGAAA
  ACACTTTCATGGATCCTGAGGACAAATGGCATCAGAGAGCAAAGCAGCTCAAGAATTACCAAGAACATCT
  GTCTCAGTACGAAGATACAAAATCAGGCAATGTCCCAAGAGGTCAGTCTTCCGATGCAGCCAATGGCCAG
  CAACCTTCCAGAAGAACTGCCAAGGCCAGGGTTCGGAAACAGAGGAAACACCAGAAAGGCCTGAAATCTC
  TTGGGACAAAAGAGCTTGTTGTCAGTCAAAATAATCAGAATAACCCTTTCCAGCAACCACAGAACCAAAG
  GCAGACTGCGGATGCCTCAGCAAAGCATGAGCTGGCTGCACAAACGAACGCTTCTAACCCCAATTTACAG
  GACGCCAGAACGCTCACCCACAATCCCAAAGTCACCTCCGACTCTTTCGTTTCACCTAAGCAGGCTTTAG
  ACAGAGCTTTATATAAAAACTCCATCTCAGGACTGAACGCTAATAAAAGACGAGGACACAGACGTGAGGA
  AGAGAGAATTCTGTATCAGCCGCAGCCCATCTACGTGTTTTCTGATACACATCTGCAGGACTTTAATGAA
  CTTCCTCACAGACACGAGTCCCCGAGCCAGAGAGCCCCTCAGTCCGATCACCACATGAACACACACAGGT
  CAACGAAGACAAAGAAGCCAGCCAAACAACCGCAGGCAGAGACGAAATACAAGAACATAGAGATGCTCTG
  GAAATTCCACTCTTCCTCAGACATGGAACCCGCGAGTGCCTCGCCAGACTCCCGGCTTGCCCAGATCATG
  GAACAGCACCAGCAAGCCTTGATGCAGCTGGCAGAGGTGCAGCCCAGCGAGGGCTCTCTGGCCAGCATCA
  TACTCCCACCTATCCTGTCCAGGGTAGAAAGTGAATCGCAGCTCAATTCAGAGAGAAGCCACAGACACCA
  GGTGAAGATGGCCCGTAGTAATTCGGAAGGCTACCTGCTTCAGCTGGAGAGAGGAAAGAGACACAGGAAG
  CGAAGCAGCACAAAGAGTTCCAAGCTGAAAGGCTATCAGAAAAGAGACGTGAAGCTTGGAGGCCTGGGAC
  CTGACTTTGCATCCGTCAGAGACAAAATGCAAATGTTAATGCAGCAAAAAGAATACGCACAGCAAGTCAA
  GGATTACAACATGAAGGCCCTGTCCATTCTGTCCAAACCACAGACATCAAAAACCGAGAGTAAATCTGCG
  ATTTCTCGGAAGAAGGCTTTGGACTATGCTAAGACCATCCCCAAACCCAAACCACCAAATCTACCTGATC
  AAACAGCAAAGAAAACTAAGAACTCAAGGCACAGTGAAAAAGAAGGAGGCTTACCAGAAATTTCCCTGTT
  GGAGATCCTGCAGAGCAGACACGAGCGGGAAAAGCAGGCTGTGGCTGCTTTCAAAGTCCTTCACATCGTC
  TAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001081121
• Insert Size: 2313 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081121.1, NP_001074590.1
• RefSeq Size: 3068 bp
• RefSeq ORF: 2313 bp
• Locus ID: 70989
• UniProt ID: E9Q793
• Cytogenetics: 9 A5.1
• Gene Summary: Required for the normal development of cilia in brain ependymal cells lining the ventricular surfaces.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (b) is shorter at the N-terminus compared to isoform a. Variants 2 and 3 both encode the same isoform (b). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.