Kirrel3 (NM_001190911) Mouse Untagged Clone

CAT#: MC221498

Kirrel3 (untagged) - Mouse kin of IRRE like 3 (Drosophila) (Kirrel3), transcript variant A, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Kirrel3
• Synonyms: 1500010O20Rik; 2900036G11Rik; mKIAA1867; NEPH2; SST4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221498 representing NM_001190911
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGACCTTTCCAGCTGGATTTGCTCTTCCTCTGCTTCTTCCTCTTCAGTCAAGAGCTTGGCCTCCAGA
  AGAGAGGATGCTGTCTGGTACTGGGCTACATGGCCAAGGACAAGTTTCGGAGAATGAATGAAGGTCAAGT
  CTACTCCTTCAGCCAGCAACCCCAGGACCAAGTGGTGGTGTCAGGACAGCCAGTGACTCTGCTGTGTGCC
  ATCCCTGAATATGATGGCTTCGTCCTGTGGATCAAAGATGGCTTGGCTCTGGGTGTAGGCAGAGACCTCT
  CAAGTTACCCCCAGTACCTGGTGGTGGGGAACCACCTCTCAGGAGAGCATCACCTGAAGATCCTGAGGGC
  TGAGCTTCAGGATGATGCCGTGTATGAGTGCCAGGCCATCCAGGCTGCCATCCGGTCCCGCCCTGCACGC
  CTCACCGTCCTGGTGCCACCAGATGACCCCATCATCCTAGGGGGGCCTGTGATCAGCCTTCGGGCAGGGG
  ACCCCCTCAACCTCACCTGCCACGCAGACAATGCCAAGCCTGCGGCTTCCATCATCTGGCTACGTAAAGG
  AGAGGTCATCAATGGAGCCACCTACTCCAAGACCCTGCTTCGAGACGGCAAACGAGAAAGCATTGTCAGC
  ACCCTCTTCATCTCCCCAGGAGACGTGGAAAATGGACAGAGTATTGTGTGCCGAGCCACCAACAAAGCCA
  TCCCCGGAGGAAAAGAGACCTCTGTCACCATAGACATCCAGCATCCACCGCTTGTCAACTTGTCCGTGGA
  ACCACAGCCGGTATTGGAGGACAACATCGTCACGTTCCACTGCTCTGCAAAGGCCAACCCAGCTGTCACC
  CAGTACAGGTGGGCCAAACGGGGTCACATCATCAAGGAGGCATCTGGGGAGCTGTATAGGACCACGGTGG
  ACTACACATACTTCTCAGAGCCTGTATCCTGTGAAGTAACCAATGCCCTGGGCAGCACCAACCTCAGCCG
  CACAGTGGATGTATACTTCGGTCCTCGAATGACCTCAGAGCCTCAGTCACTGCTGGTAGATCTGGGCTCC
  GATGCTGTCTTCAGCTGTGCGTGGATCGGCAACCCGTCTCTGACCATCGTGTGGATGAAACGAGGTTCTG
  GTGTGGTCCTGAGCAATGAAAAGACCCTAACCCTCAAATCTGTCCGCCAAGAGGATGCTGGGAAGTACGT
  GTGCCGGGCTGTGGTGCCCCGGGTAGGAGCTGGGGAGAGAGAGGTGACCTTGACTGTCAATGGACCCCCC
  ATCATCTCCAGCACACAGACCCAGCACGCCCTCCACGGAGAGAAGGGCCAGATCAAATGCTTCATCCGGA
  GCACACCACCGCCTGACCGAATTGCCTGGTCCTGGAAGGAGAATGTGCTGGAGTCAGGGACATCAGGGCG
  CTACACAGTGGAGACGGTGAACACGGAGGAGGGAGTCATCTCCACATTGACCATTAGCAACATTGTGCGT
  GCTGACTTCCAGACCATATACAACTGTACAGCCTGGAACAGCTTTGGCTCTGACACAGAGATCATCCGAC
  TCAAGGAACAAGGTTCGGAAATGAAGTCGGGAGCCGGGCTGGAAGCAGAGTCTGTACCAATGGCCGTCAT
  CATCGGGGTGGCCGTAGGAGCTGGCGTGGCCTTCCTCGTCCTAATGGCAACCATTGTGGCCTTCTGCTGT
  GCCCGTTCCCAGAGAAATCTCAAAGGTGTTGTATCAGCCAAAAATGATATTCGAGTGGAAATTGTGCACA
  AGGAGCCATCTTCTGGCCGGGAGGCTGAGGACCACACCACCATAAAGCAGCTGATGATGGACCGGGGTGA
  ATTCCAACAAGACTCGGTGCTGAAACAGCTGGAGGTCCTCAAAGAAGAGGAGAAGGAGTTTCAGAACCTG
  AAGGACCCCACCAACGGCTACTACAGCGTCAACACCTTCAAAGAACACCATTCAACTCCAACCATCTCCC
  TGTCCAGCTGCCAGCCAGACCTGCGTCCGACAGGCAAACAGCGTGTGCCCACAGGCATGTCCTTCACCAA
  CATCTACAGCACCTTGAGCGGCCAGGGCCGCCTCTACGACTATGGACAGAGGTTTGTGCTGGGCATGGGC
  AGCTCTTCCATTGAGCTTTGTGAGCGGGAGTTTCAGAGGGGCTCCCTCAGCGACAGCAGCTCCTTCCTGG
  ACACGCAGTGTGACAGCAGCGTCAGCAGCAGCGGCAAGCAAGATGGCTACGTGCAGTTTGACAAGGCCAG
  CAAGGCTTCTGCCTCCTCTTCCCACCATTCCCAGTCCTCTTCCCAGAACTCCGACCCCAGCCGACCCCTG
  CAGCGGCGGATGCAGACTCACGTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001190911
• Insert Size: 2337 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001190911.1, NP_001177840.1
• RefSeq Size: 3737 bp
• RefSeq ORF: 2337 bp
• Locus ID: 67703
• UniProt ID: Q8BR86
• Cytogenetics: 9 A4
• Gene Summary: Synaptic adhesion molecule required for the formation of target-specific synapses (PubMed:23637329, PubMed:26575286). Required for formation of target-specific synapses at hippocampal mossy fiber synapses. Required for formation of mossy fiber filopodia, the synaptic structures connecting dentate granule and GABA neurons. Probably acts as a homophilic adhesion molecule that promotes trans-cellular interactions and stabilize mossy fiber filipodia contact and subsequent synapse formation (PubMed:26575286). Required for the coalescence of vomeronasal sensory neuron axons (PubMed:23637329). May be involved in the hematopoietic supportive capacity of stroma cells; the secreted extracellular domain is directly responsible for supporting hematopoietic stem cells (PubMed:12665856).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (A) encodes the longest isoform (A).