Kifap3 (NM_010629) Mouse Untagged Clone

CAT#: MC221482

Kifap3 (untagged) - Mouse kinesin-associated protein 3 (Kifap3), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Kifap3
• Synonyms: KA; KAP-3; KAP3; SMAP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221482 representing NM_010629
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAAGGCGAGGACGCCAGATACCTCAAAAGGAAAGTTAAAGGGGGGAATATTGATGTACATCCATCAG
  AAAAAGCTCTCATTGTTCAGTATGAAGTGGAAGCTACCATTCTTGGAGAAATGGGAGATCCCATGTTGGG
  AGAACGAAAGGAATGCCAAAAAATCATCCGTCTGAAGAGTCTCAATGCTAACACAGACATCACCTCTCTG
  GCCCGGAAGGTGGTTGAGGAATGTAAGCTCATCCATCCCTCAAAGCTAAGCGAGGTGGAGCAGCTTTTGT
  ACTATCTACAGAACCGCCGAGACTCATTGCCGGGAAAAGAAAAAAAAGAAAAATCAAGCAAGCCTAAAGA
  CCCACCTCCTTTTGAAGGGATGGAGATTGACGAAGTGGCCAACATTAATGACATGGACGAATACATTGAG
  CTCTTGTATGAAGACATTCCAGATAAGGTTCGGGGTTCTGCCTTGATCCTACAGCTTGCTCGAAATCCTG
  ATAACCTGGAAGAGCTATTATTAAATGAAACTGCCCTGGGTGCGCTAGCAAGAGTCCTGAGAGAAGACTG
  GAAACAAAGTGTTGAGTTAGCTACAAACATCATTTATATCTTCTTTTGCTTTTCTAGCTTTTCTCATTTT
  CATGGACTCATCACTCACTACAAAATTGGAGCACTGTGTATGAATATCATTGATCATGAGTTAAAAAGAC
  ACGAGCTTTGGCAAGAAGAACTCTCTAAGAAGAAGAAAGCTGTTGATGAAGACCTTGAAAATCAAACATT
  GAGAAAGGATTATGACAAAACCTTTAAAAAATACCAAGGACTTGTGGTAAAACAAGAGCAGCTACTGAGA
  GTTGCTCTCTACTTGCTTTTGAATCTTGCGGAGGACACACGTACAGAACTGAAGATGAGGAACAAGAATA
  TCGTTCACATGTTGGTGAAGGCTCTTGATCGGGACAATTTTGAGCTGCTGATTCTGGTCGTGTCATTCTT
  AAAGAAACTGAGTATTTTTATGGAGAATAAAAATGACATGGTAGAGATGGATATTGTTGAAAAACTGGTA
  AAAATGATACCCTGTGAGCATGAAGATCTCCTGAATATCACCCTCCGGCTCCTCTTAAACCTCTCGTTTG
  ACACAGGCCTGAGGAACAAGATGGTACAAGTTGGGCTTCTCCCAAAGCTCACTGCACTCCTGGGCAATGA
  AAACTACAAACAAATAGCAATGTGTGTGCTTTACCATATAAGCATGGATGACCGCTTTAAGTCAATGTTT
  GCATATACTGACTGCATACCACAGTTAATGAAGATGCTCTTTGAATGTTCAGATGAACGAATTGACTTGG
  AGCTGATTTCTTTCTGCATTAATCTTGCTGCTAACAAGAGAAATGTCCAGCTCATCTGTGAAGGAAATGG
  GCTGAAAATGCTCATGAAAAGAGCTCTGAAGCTCAAGGACCCACTGCTGATGAAGATGATCAGAAACATC
  TCCCAGCATGATGGGCCCACTAAGAATTTGTTCATTGATTATGTTGGGGACCTTGCAGCCCAGATTTCCA
  GTGATGAAGAGGAGGAGTTTGTAATCGAGTGTTTGGGAACGCTGGCAAATCTGACAATCCCAGATCTAGA
  CTGGGAACTGGTCCTGAAGGAGTACAAGCTGGTTCCATTCCTCAAAGACAAACTAAAGCCAGGTGCCGCA
  GAGGATGACCTTGTTTTAGAAGTGGTTATCATGATTGGGACGGTGTCTATGGATGACTCTTGTGCTGCCC
  TACTGGCCAAATCTGGGATAATCCCAGCCCTCATCGAGCTGCTGAACGCTCAACAAGAAGATGATGAATT
  TGTGTGTCAAATAATCTATGTCTTCTACCAGATGGTGTTCCATCAGGCCACAAGAGATGTCATAATCAAG
  GAAACACAAGCTCCAGCATATCTCATTGACCTGATGCATGATAAAAATAATGAAATCCGGAAAGTCTGTG
  ATAACACATTAGATATCATCGCAGAGTATGATGAAGAGTGGGCCAAGAAAATTCAGAGTGAGAAGTTTCG
  CTGGCATAACTCTCAGTGGCTGGAGATGGTGGAGAGCCGTCAGCTGGATGAGAGCGAGCAGTACTTGTAT
  GGTGATGATCGCATTGAGCCGTACATCCATGAAGGGGACATTCTTGAAAGGCCTGACCTTTTCTACAACT
  CAGACGGACTAATTACCTCTGAAGGAGCCATAAGTCCAGACTTCTTCAATGATTTTCACCTCCAGAATGG
  AGATGTGGTGGGGCAACACGCATTTCCTGGCAGCACTGTCCATCCAAGGATTTCAAAGTGCTTTGCAAGT
  GTGCACTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_010629
• Insert Size: 2319 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010629.3, NP_034759.1
• RefSeq Size: 4027 bp
• RefSeq ORF: 2319 bp
• Locus ID: 16579
• UniProt ID: P70188
• Cytogenetics: 1 H2.1
• Gene Summary: The protein encoded by this gene is the non-motor subunit of kinesin-2 complex, and forms a heterotrimer with two members of the kinesin superfamily of proteins that together form a microtubule plus-end directed translocator that plays an important role in intracellular transport, mitosis, and cell-cell adhesion. This protein contains multiple armadillo repeats involved in protein binding, and may serve as an adaptor to regulate binding of cargo with the motor proteins. Conditional disruption of this gene in mouse neural precursor cells caused a tumor-like phenotype and defective organization of the neuroepithelium thought to be the result of altered N-cadherin subcellular localization. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2015]
  Transcript Variant: This variant (2) contains an alternate exon in the 3' coding region and differs in its 3' UTR, compared to variant 1. The encoded isoform (KAP3B, PMID:8710890) is shorter and has a distinct C-terminus, compared to KAP3A.