Adam28 (NM_001048175) Mouse Untagged Clone

CAT#: MC221479

Adam28 (untagged) - Mouse a disintegrin and metallopeptidase domain 28 (Adam28), transcript variant 4, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Adam28
• Synonyms: C130072N01Rik; D430033C21Rik; Dtgn; Dtgn1; eMDCII; MDC-; MDC-L; MDCL; TECADAM
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221479 representing NM_001048175
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGCAATGGAGTCTTCTGGTAGTCTCTTTCCTTCTTTCTCCAGTTCCAGTAAGTGCAATAAAAGAAC
  TCCCTAAAGCCAAGAAATATGAAGTGGTTTATCCCATAAGACTTCATCCATTGCGTAAAAGAGAGACCCA
  AGAGCCAGAGCCAAAGGAAACATTTGAAACTGAGCTAAGGTACAAAATGACAGTAAATGGAAAGGTTGCT
  GTGCTGTATCTGAAGAAGAACAACAAGCTCCTTGCGCCTGACTACTCGGAAACATACTATAATTCCAGTG
  GAAACAAGGTCACCACAAGCCCGCAAATCATGGATAGCTGTTACTACCAAGGACACATCGTAAATGAGAA
  AGTTTCTGCAGCCAGCATCAGCACCTGTCAAGGACTACGGGGTTACATCAGTCAAGGAGATGAAAAGTAT
  TTTATCGAACCTTTGAGCTCGGAGAACTTGGATGAACAGGCACATGCACTCTTCAAGGACGACTCCAATG
  AAGACCAGGAGAAGAGCAACTGTGGTGTGGATGATGCGCTATGGCTCCAAGGGCTGCATCAGGACGTGGC
  CCTCCCTGCCACCAGGTTGATTAAGTTGAATGATGGGATGGTTCAAGAACCTAAGAAATACATAGAATAT
  TATGTGGTCCTGGATAATGGTGAGTTTAAGAAATACAATAAAAATCTTGCTGAAATACGAAAGATAGTGC
  TTGAGATGGCCAATTACATCAACATGCTTTACAATAAGCTTGATGCCCACGTGGCCTTAGTTGGAGTGGA
  AATCTGGACCGATGGGGATAAAATAAAGATAACACCAGATGCCAACACCACCCTGGAAAACTTCTCTAAG
  TGGAGGGGAAATGATCTGCTAAAACGAAAGCATCATGATATTGCCCAGCTAATCTCATCAACAGACTTTT
  CTGGATCAACAGTGGGTCTAGCCTTCATGTCGTCGATGTGTTCACCTTACCATTCTGTTGGCATTGTTCA
  GGACCACAGTAACTACCATCTTCGAGTCGCAGGAACAATGGCTCATGAAATGGGTCACAATCTTGGCATG
  ATTCATGACTACTTGAGCTGCAAGTGTCCATCTGAAGTCTGTGTAATGGAGCAGTCACTAAGGTTCCATA
  TGCCTACAGACTTCAGCTCCTGCAGTCGTGTCAATTACAAACAGTTTCTTGAAGAAAAATTATCGCATTG
  CCTCTTTAATAGCCCATTGCCATCAGATATCATATCCACCCCAGTCTGTGGGAACCAGTTGTTGGAAATG
  AATGAGGACTGTGACTGTGGCACACCCAAGGAGTGTACTAACAAATGCTGTGATGCAAGGACCTGTAAAA
  TTAAAGCAGGTTTCCAGTGTGCCCTGGGGGAATGCTGTGAGAAATGCCAACTTAAAAAACCTGGGGTTGT
  GTGCAGAGCAGCAAAAGATGAGTGTGATCTGCCTGAAGTGTGTGATGGTAAATCCAGCCACTGCCCAGGT
  GACAGATTCAGAGTCAATGGCTCCCCTTGCCAAAATGGGCATGGTTACTGCTTGAAGGGCAAATGTCCCA
  CCCTGCAGCAGCAGTGCATGGACATGTGGGGTCCAGGAACCAAGGTTGCAAATACATCATGTTACAAGCA
  GAATGAAGGTGGGACAAAGTACGGATACTGTCATGTGGAGAATGGCACACACATGCCCTGCAAAGCAAAA
  GATGCCATGTGTGGGAAATTGTTCTGTGAAGGCGGATCAGGTGATTTGCCCTGGAAAGGACTTACCATAT
  CTTTCCTGACATGTAAATTATTTGATCCTGAAGACACAAGTCAAGGAGTAGACATGGTGGCCAATGGAAC
  CAAGTGTGGAACTAACAAGGTGTGCATTAATGCTGAGTGTGTGGACATGGAGAAGACTTACAAGTCAGCC
  AACTGCTCCTCAAAGTGCAAGGGGCACGCAGTGTGTGACCATGAGCTTCAGTGTCAGTGCAAGGAAGGAT
  GGGCCCCTCCTGACTGCGAGAATTCAGCCACAGTCTTCCACTTCTCCATCGTGGTTGGCGTGCTTTTCCC
  CCTAGCAGTCATATTTGTGGTGGTTGCTATAGTGATCCAGCGCCAAAGTGCCAGAAGGAAGCAGAGGAGA
  GTTCAGAGGCTACCATCCACCAAGGATGCCAAGCTACACAATCAGAAGTGTAGACCCCAAAAGGTGAAGG
  ATGTTCAACCCCAGGAGATGAGTCAGATGAAAAAGCTCCATGTGTCTGATCTGCCCTCTGAAGAGCCGGA
  GCCTCCACCTGATGTCCTAATCACAAAGCCAAATTTCCCACCACCACCAATTCCTGTTTCCTTGGACCCA
  AATGCAAAAGTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001048175
• Insert Size: 2325 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001048175.2, NP_001041640.1
• RefSeq Size: 4054 bp
• RefSeq ORF: 2325 bp
• Locus ID: 13522
• UniProt ID: Q9JLN6
• Cytogenetics: 14 D1
• Gene Summary: This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are typically membrane-anchored, although a form of this protein may be secreted. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate a mature protein product. This protein may bind to integrins and regulate lymphocyte migration by enhancing cell adhesion. [provided by RefSeq, Aug 2015]
  Transcript Variant: This variant (4) includes an alternate terminal 3' exon and its transcription extends past a splice site that is used in variant 1, resulting in a novel 3' coding region and 3' UTR compared to variant 1. The encoded isoform (4) has a distinct and shorter C-terminus, compared to isoform 1. It is not known whether this isoform (4) is proteolytically processed in the same manner as isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.